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Biomedical subjects

N A Kaufmann

Publications and source records attributed to N A Kaufmann.

At least 37 records · Page 2Linked to original sources

Reported physical activity and blood lipids in Jerusalem adults.

The association of physical activity and fasting plasma lipids and lipoproteins was studied in an adult Jerusalem population consisting of 1,095 men and 614 women. Participants were questioned about habitual physical activity, and the relationship of their reported levels of activity of fasting lipid and lipoprotein values was examined. After adjustment was made for age, ethnic origin, body mass, season, cigarette smoking and alcohol consumption, the following associations were found: 1) an inverse association of reported physical activity with triglyceride, total cholesterol and very low-density lipoprotein-cholesterol (not significant), and 2) a significant positive association between reported activity and high-density lipoprotein-cholesterol in men. These findings generally confirm results found in other Western populations. Too few women reported moderate or strenuous physical activity to permit an examination of lipid associations in women.

Adult↗

Nutrient intake in Jerusalem--consumption in 17-year-olds.

The food intake of 17-yr-old Jerusalem residents was assessed in a random sample, including 627 males and 551 females, by a 24-h dietary recall method. The intake of energy in boys and girls was lower than the recommended energy intake for this age, but the intake relative to body weight was low only in girls. In boys and girls, the intakes of fat relative to energy were 32.4 and 33.7%, respectively; those of saturated fatty acids (SFA) (9.8 and 10.5%) were lower and those of carbohydrates (53.9 and 52.5%) were higher than intakes found in other Western countries. The P:S (polyunsaturated to saturated fatty acids) ratio of their diets was high and ranged from 0.79 to 1.29 in various sex and origin groups. Intake of cholesterol per 1,000 kcal was similar to that in Western countries. There were marked differences in nutrient intake of subjects whose fathers had immigrated from different countries. Boys whose fathers were born in Israel or Europe had higher intakes of fat and cholesterol, and both boys and girls had a higher intake of SFA and a lower intake of carbohydrates and starch as well as a lower P:S ratio than did their counterparts whose fathers were born in Asia and North Africa. Thus, the nutrient intake of youngsters in Jerusalem differs markedly from that of populations in other Western countries.

Adolescent↗

Nutrient intake in Jerusalem--consumption in adults.

During 1976-80, nutrient intake was assessed in a cross-sectional sample of 1,589 middle-aged Jerusalem residents (1,028 males and 561 females) using the protocols and methods of the North American Lipid Research Clinics (LRCs) Prevalence Studies. All subjects were parents of 17-yr-old youngsters studied previously. Energy intake in both males and females was lower than the recommended energy intake for this age-group. Intake of fat relative to energy in males and females was 32.1 and 34.0%, of saturated fatty acids (SFA) 10.0 and 11.0%, and of total carbohydrates 50.2 and 49.9%, respectively. Cholesterol consumption was 210 and 238 mg/1,000 kcal in males and females, respectively. The mean polyunsaturated to saturated fatty acids (P:S) ratio of the diet was 0.95 in males and 0.84 in females. There were marked differences in the nutrient intake of subjects of different ethnic origin. Subjects from Israel and Europe consumed more total fat and SFA and less total carbohydrates than did their counterparts from Asia and North Africa. Consumption of polyunsaturated fatty acids (PFA) was highest in subjects from Israel. The P:S ratio of the diet was highest in the North African group, followed by the Israeli group, and lowest in subjects from Europe. The highest sucrose intake was found in subjects from North Africa and the highest cholesterol intake in immigrants from Europe. Nutrient intake of Jerusalem adults differed markedly from that of populations in other Western countries.

Adult↗

Nutrient intake in Jerusalem--effects of origin, social class and education.

The independent association of ethnic group, social class and education with nutrient intake was studied in a sample of 1,294 adults in the Jerusalem Lipid Research Clinic (LRC) population. By univariate analysis, intake of fat and saturated fatty acids (SFA) was higher (P less than or equal to 0.05) in males and females of the upper social classes (classified by the occupation of the head of the family) than in the lower classes, while the opposite trend was found for the consumption of carbohydrates and starch. In men, an association between social class and the intakes of protein and other carbohydrates (i.e., other than sucrose and starch) and the ratio of polyunsaturated fatty acids (PFA) to SFA (P:S ratio) was also found. In both sexes, the mean intakes of SFA and other carbohydrates were higher and that of starch lower in subjects with a higher level of education (P less than or equal to 0.05). Education was also associated with the consumption of protein and fat in males and with that of carbohydrates and sucrose in females. Country of origin was related (P less than or equal to 0.05) to the intake of fat, SFA and other carbohydrates in both sexes, to that of protein and cholesterol in males and to that of carbohydrates, sucrose and starch in females. The P:S ratio of the diet of male subjects was also associated with origin. Using various models of analysis of variance, it was shown that origin was associated with nutrient intake (P less than or equal to 0.10), independent of the effect of social class and education for protein, fat, SFA, cholesterol, sucrose and other carbohydrates in males, and for fat, SFA, PFA and other carbohydrates in females. The P:S ratio of the male diet was also associated with origin. The level of education was independently related (P less than or equal to 0.10) to the intake of fat, SFA, starch and other carbohydrates in males and to that of sucrose in females, while social class was associated independently with carbohydrate consumption in males only. After prior adjustment for origin, education had a stronger residual effect than did social class in males, while in females the associations of social class and education with nutrient intake were almost identical.

Adolescent↗

Correlates of biochemical variables in the Jerusalem Lipid Research Clinic population.

The associations of sex, age and ethnic origin with hematocrit and biochemical variables were studied in the Visit 2 population of the Jerusalem Lipid Research Clinic--1,709 adults and 1,248 17-yr-olds. Differences in globulin, alkaline phosphatase, creatinine and uric acid levels were statistically significant by ethnic origin in both youngsters and adults. Differences by sex and age in many of the variables studied suggested hormonal effects that were more pronounced before the age of menopause. The data presented, relating to a multiethnic free-living population, may provide a useful reference for physicians in this region for distributions of these variables in the population.

Adolescent↗

Comparison of weight loss on low-calorie (800-1200) and very-low-calorie (300-600) diets.

Conventional food diets of 300-600 kcal (14-20 days) were not as efficient as anticipated in increasing the rate of weight loss in ten obese patients previously given diets of 800-1200 kcal (13-30 days). The greatest efficiency was 76 per cent of the theoretical. The final weights after refeeding the 800-1200 kcal diets (9-14 days) were 0.5-2.0 kg greater than expected to result from the increased caloric deficit, and close to the weights that would have been reached had the 800-1200 kcal diets been continued without increased reduction in caloric intake.

Body Weight↗

Bilirubin binding capacity of albumin isolated from cord-blood serum is less than that from serum of adults.

As estimated by Sephadex gel filtration, the bilirubin binding capacity of albumin isolated from cord-blood serum by ion-exchange chromatography is less than that of albumin isolated from serum of adults. Albumin isolated from cord-blood serum showed increased bilirubin binding as compared with the albumin in the native serum. These findings suggest that the lower bilirubin binding capacity of serum from newborns as compared with serum from adults is a result of both an intrinsic deficiency in binding capacity of neonatal albumin and the presence of substances in neonatal serum that interfere with bilirubin binding.

Adult↗

Biochemical parameters of vitamin D nutriture in old people in Jerusalem.

Serum 25-hydroxycholecalciferol (25[OH]D3) levels and other parameters of vitamin D nutriture were examined in 58 subjects aged 70 or more, living in Jerusalem. They were compared with those of 54 young adults living in the same neighbourhood. No evidence was obtained of a lower level of vitamin D nutriture in the elderly compared to younger adults. Serum 25 (OH)D3 of the elderly adults was 18.4 (SEM: 1.4) ng/ml and in the younger adults, 17.8 (1.0) ng/ml. There was no seasonal variation in serum 25(OH)D3, nor could a strong association be found between reported vitamin D intake nor with exposure to sunshine. There was a negative correlation between serum alkaline phosphatase and the calcium-phosphorus product in serum. High values of alkaline phosphatase were associated with reported low exposure to sunlight and, in elderly persons, with a reported low consumption of vitamin D.

Adult↗

Attitudes of adolescents to their body build and the problem of juvenile obesity.

Prevalence of obesity, attitude to body weight and dimensions, eating habits, opinions on good nutrition and on the causes and prevention of obesity were studied in two groups of Israeli children, each comprising about 500 boys and girls, 13--14 years old. Mean relative weight was close to median weight for height, although 9 per cent of both boys and girls weighed more than 120 per cent of median weight. Weight was closely related to triceps skinfold thickness. Twenty-six per cent of the boys and 15 per cent of the girls rated themselves as thin, 61 per cent of children of both sexes as medium, and 13 per cent of the boys and 25 per cent of the girls as obese. Self-perception of body build was more closely related to weight than to skinfold thickness. Two-thirds of the boys and over one-half of the girls were satisfied with their weight, but dissatisfaction with size and shape was often expressed. Boys wished to have larger muscles and chest circumference and many girls, even when not obese, wanted to be smaller in size and shape. Most of the obese children wanted to lose weight. Of 499 children examined, 16 boys (7 per cent of the sample) and 58 (21 per cent) girls reported for dietary therapy. While most of these children were obese, by either subjective or objective criteria, an appreciable number of nonobese children were also dieting. The children's own perception of their body build seemed to be a strong motivation to diet for weight control. Most of the dieters did not receive any dietary advice from professional people. Over two-thirds of both boys and girls believed that daily consumption of milk, bread, fruits, eggs, cheese, meat, and tomatoes is desirable. More overweight than thin and normal-weight children indicated that, to prevent obesity, all kinds of food are permissible, but only in limited amounts. Most children believed in the fattening value of cakes, sweets, fried and fatty foods, potatoes, bread and nuts. Overweight children, particularly girls, reported eating less bread, cake and cream, adding less sugar to beverages, and eating sweets and ice cream less frequently than thin and normal-weight children. Overweight teen-agers appear to be more conscious of their food intake than under- and normal-weight children.

Adolescent↗

The validity of biochemical assessment of thiamine, riboflavin and folacin nutriture.

Nutriture of thiamine, riboflavin and folacin was assessed by two tests: thiamine (109 subjects) by the TPP effect of erythrocyte transketolase activity and urinary excretion of thiamine (mug/g creatinine); riboflavin (81 subjects) by the activation coefficient of erythrocyte glutathione reductase and excretion of riboflavin in urine (mug/g creatinine); and folacin (91 subjects) by estimation of folacin in red blood cells and in serum (ng/ml). The following correlation coefficients (r) were obtained: transketolase activity vs thiamine excretion: -0.33; glutathione reductase vs riboflavin excretion: -33; and red blood cell folacin vs serum folacin: 0.77. When "deficient" and "low" values were defined as "not acceptable" and compared with "acceptable" values, sensitivity of thiamine excretion was 54%, of riboflavin excretion 33% and of serum folacin level 90%. The respective value of specificity were 75%, 83% and 37%. Sensitivity of thiamine excretion and of serum folacin level, respectively, increased when stricter criteria of insufficiency were applied and assessment of "deficiency" of these vitamins rather than of "non-acceptability" was attempted.

Aged↗

The redox state and the concentration of ketone bodies in tissues of rats fed carbohydrate free diets.

The effect of carbohydrate free-low protein diets on the redox potential and ketone body concentrations in liver, muscle, and blood was investigated. Two carbohydrate free diets were fed: 1) A diet in which all the non-protein energy was provided by fatty acids (FA); 2) A similar diet in which the fatty acids were substituted by neutral fat (NF). A carbohydrate rich diet (HC) was fed for comparison. The redox potentials in cytoplasma and mitochondria were calculated from the relative concentrations of the [lactate]:[pyruvate] and [beta-hydroxy-butyrate]:[acetoacetate] couples respectively. In fed rats the cytoplasmic redox potential in liver was much higher in FA than in NF rats, but in fasted NF rats it increased markedly and equaled that of FA rats. In liver mitochondria the redox potential was lower in fed FA than in fed NF rats, while after a 24 hour fast it increased in FA rats and decreased in NF rats. Total ketone body concentrations were high in fed NF rats and increased further in fasted rats, however, in FA rats the high concentrations in the fed state decreased after the short fast. The changes of total ketone-bodies in blood and muscle followed those in liver in both fed and fasted states.

Acetoacetates↗

Experience with Sephadex gel filtration in assessing the risk of bilirubin encephalopathy in neonatal jaundice.

Clinical experience with Sephadex gel filtration for evaluation of the bilirubin binding affinity of serum in neonatal jaundice is reported. In serum specimens from 362 jaundiced neonates the results of the test were in most cases in good agreement with the independently determined decision to perform exchange transfusion. In all relevant cases of the 24 with bilirubin encephalopathy the Sephadex test was strongly positive. The test was positive at lower bilirubin levels and bilirubin:albumin molar ratios in preterm as compared with term neonates, especially those in poor clinical conditions. Among term infants the test indicated an increase in bilirubin binding affinity after the fifth day of life. The Sephadex test appears to be of practical value in supplementing and refining existing criteria for exchange transfusion.

Bilirubin↗

Characteristics of a photoproduct of bilirubin found in vitro and in vivo, and its effect on the bilirubin binding affinity of serum.

The 430 pigment isolated from bilirubin-albumin solutions illuminated in vitro, decreased the bilirubin binding affinity of serum. This effect was demonstrated for both tightly and loosely bound bilirubin in serum, but in albumin solutions pretreated with charcoal only the binding of loosely bound bilirubin was decreased. This finding is of importance because a similar pigment has been isolated from the body fluids of an infant with Crigler-Najjar syndrome. The production and accumulation of 430 pigment during phototherapy might decrease the binding of bilirubin to albumin in the circulation.

Albumins↗

Weight and skinfold thickness changes during a physical training course.

The effect of an intensive physical training program on body weight and skinfold thickness was compared in subjects who had initially small (group S), medium (group M) and large (group L) skinfold thickness representing mostly thin, normal and obese recruits, respectively. Weight and skinfold thickness at three sites (triceps, subscapula and abdomen) were measured at the beginning and end of a 6 week intensive military training program. Subjects were on an ad-lib diet. Seventy-one percent of the subjects in group S increased their body weight and 60% increased the sum of their skinfold thickness (SSFT) at the three sites during the training period. These increases were significant at P less than 0.001 and P less than 0.01 for weight and SSFT respectively. In groups M and L the trends for changes in SSFT were in the opposite direction, with 68% of the former and 98% of the latter showing decreases in SSFT. The mean decreases were significant at P less than 0.02 and P less than 0.001 for groups M and L respectively. While the subjects in group L lost weight significantly (P less than 0.001), those in group M reduced their weight only slightly. Our data indicate that changes in body fat, indicated by changes in SSFT, during several weeks of physical activity are not unidirectional and are related to the initial skinfold thickness.

Body Composition↗

Unsaturated fatty acids in adipose tissue of Israeli Jews.

The ratio of polyunsaturated to saturated fatty acids in the adipose tissue of Israelis was found to be 0.88 for Ashkenazic Jews and 1.13 for non-Ashkenazic Jews, the highest reported for any population on a free-choice diet. Despite the unusually high ratios, indicating a high consumption of polyunsaturated fat, there is nevertheless, a relatively high incidence of ischemic heart disease among Israelis.

Adipose Tissue↗

Increase in bilirubin-binding affinity of serum with age of infant.

The bilirubin-binding affinity of infant serum, as determined by Sephadex gel filtration, began to increase during the first week of life and continued to increase gradually until the age of 5 months, when it reached that of adult serum. This increase might be due to a transition from a fetal to an adult type of serum albumin and/or to a decrease in the concentration in serum of the substances thought to compete with bilirubin for binding to albumin.

Age Factors↗