The composition of cytochromes was studied in Photobacterium fischeri 6 at different growth phases and under various conditions of cultivation. The electron transport chains of the bacterium are characterized by the presence of cytochromes b and c types. The terminal oxidases are cytochromes o, a2+a1 and P-450. The hemoprotein P-450 functions as a mixed function oxidase. The qualitative composition of cytochromes does not depend on the growth phase of the bacterium but does on the conditions of cultivation. The bacterium synthesizes cytochromes a2+a1 in the conditions of oxygen deficiency. The synthesis of cytochrome o depends on the composition of the medium.

Culture Media↗

PubMedDate not supplied

[Cytochromes of the luminescent bacterium, Photobacterium fischeri, their solubilization and relationship to luminescence].

The hemoprotein composition of the luminescent bacterium Photobacterium fischeri was studied, in particular, the distribution of cytochromes among the bacterial fractions, viz. cell-free extract, supernatant, "particles", protein preparation. The hemochromogenic analysis has shown that the principal hemoproteins of Photobacterium fischeri are cytochromes, with hemes of the b and c type. The activity of luciferase is distributed with hemoproteins. The purified preparation of luciferase contains cytochrome of the b type that has been identified as mixed functions oxidase--P-450.

Cytochromes↗

PubMedDate not supplied

[The effect of lovastatin on sterol synthesis and yeast resistance to polyene antibiotics].

Lovastatin (monocolin K) is a competitive inhibitor of 3-hydroxy-3-methylglutaryl-CoA-reductase. Its influence on the growth of a lovastatin sensitive strain of Rhodotorula rubra, the biosynthesis of ergosterol and the resistance to polyenic antibiotics was studied. It was shown that the lovastatin action on the strain depended on the inhibitor dose. In a concentration of 0.1 to 0.5 micrograms/ml lovastatin inhibited the yeast growth and ergosterol biosynthesis. Higher concentrations of the inhibitor in the medium led to the recovery of the sterol biosynthesis. It was also demonstrated that a decrease of the ergosterol level in the cells under the influence of lovastatin resulted in the development of resistance in the yeast to polyenic antibiotics such as nistatin and amphotericin B. Correlation between the ergosterol level in the yeast cells and their susceptibility to the polyenic antibiotics was observed.

Anti-Bacterial Agents↗