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Biomedical subjects

Mushtaq Ahmed

Publications and source records attributed to Mushtaq Ahmed.

7 recordsLinked to original sources
PubMed2025

Biogenic Synthesis and Characterization of Hypecoum pendulum Mediated Silver Nanoparticles: Revealed Outstanding Anticancer and Genotoxic Potentials.

Fabrication of silver nanoparticles by green approach is the most effective and eco-friendly technique in recent technologies. The current study aimed to generate a simple, valid, and justifiable method for biogenic synthesis of silver nanoparticles (HP-AgNPs) using aqueous extract of Hypecoum pendulum L.(HP) and to assess their in vitro anticancer and genotoxic potentials on baby hamster kidney cell (BHK-21) and human blood lymphocytes using 3-(4,5-dimethylthiazol-2-yl-)-2,5-di-phenyltetrazolium bromide (MTT) and alkaline comet assay, respectively. HP-AgNP characterization was done using UV-vis spectrometry, EDX, SEM, XRD, and FTIR techniques. The crystalline nature of HP-AgNPs with a particle size of 36.3 nm was assessed using the XRD technique. The surface morphologies with a particle size of 80 nm were verified by SEM analysis. UV spectroscopy verified the existence of HP-AgNPs by yielding a sharp peak at 417 nm with an absorbance intensity of 1.54. FTIR assessment revealed the existence of different functional moieties that contribute to the HP-AgNPs stabilization and reduction. Similarly, EDX analysis revealed Ag as a principal element (49%). MTT assay showed significant cytotoxicity by Doxorubicin and HP-AgNPs with a smaller IC50 value of 104.21 ± 4.33 and 134.91 ± 6.33 μg/mL correlated to HP extract (229.84 ± 4.66 μg/mL). The outcomes of the comet assay revealed potential DNA damage in a positive trend with concentration (25-600 μg/mL). HP-AgNP-treated lymphocytes showed higher DNA damage as compared to HP extract-treated cells, but less damage as compared to a positive control, H2O2. These outcomes showed that HP-AgNPs have demonstrated promising anticancer and genotoxic action than HP extract due to their size and shape.

Silver↗
PubMed2003

Itemized bill: novel method to audit the process of laparoscopic cholecystectomy.

One of the recurring obstacles to the successful completion of a medical audit cycle is the unavailability of accurate and complete information. This is particularly evident in the review of clinical processes, where the hand-written medical record is the source of information. We have attempted to bypass this information deficit by using information recorded primarily for financial transactions, using the itemized bill. The study was conducted in two parts. Initially information recorded as an itemized bill for the in-hospital process of laparoscopic cholecystectomies over a one-year period was analyzed. Areas for change in practice were identified, and recommendations were developed. These recommendations were presented to a multidisciplinary group consisting of consultants, residents, and nursing staff involved in caring for these patients. A clinical pathway was developed and implemented from these recommendations. One year after introduction, a review of the clinical pathway was undertaken using the same methodology. The in-hospital process consisted of 23 charge categories according to the itemized bill. Of these, 8 accounted for 95% of the total charge. The surgeon's fee and the anesthesiologist's fee accounted for 34% of the total; medical/surgical supplies, 20%; operating theater time, 17%; pharmacy, 7%; radiology, 5%; laboratory, 7%; and ward, 4%. Areas were identified in the latter 6 categories for change. Review of practice 1 year after implementation of the changes showed that a large number of recommendations were in place. We describe a new method for auditing the processes of medical care, using the itemized bill to adapt and use information primarily recorded for financial purposes.

Cholecystectomy, Laparoscopic↗
PubMed2003

Liver cyst with inflammatory hepatic duct polyp.

This is a case report of a 50-year-old woman, who presented with upper abdominal pain, vomiting and two episodes of jaundice in five months. Computerised tomographic scan revealed a cyst in the 4th segment of the liver. Peroperatively it was found to be a simple liver cyst arising from the left hepatic duct. The cyst was excised. Peroperative cholangiogram revealed obstruction in the common hepatic duct. A polyp in the common hepatic duct, causing obstruction, was excised. The presence of liver cyst with hepatic duct polyp is a rare association.

Bile Duct Neoplasms↗
PubMed2003

Surgical wound infection surveillance in general surgery procedures at a teaching hospital in Pakistan.

BACKGROUND: A surveillance system was established at the Aga Khan University Hospital in Karachi, Pakistan, to determine surgical wound infection (SWI) rates, trends, and risk factors; and to compare rates with those reported by the National Nosocomial Infection Surveillance (NNIS) system of the Centers for Disease Control and Prevention. METHODS: Surveillance was performed from January 1997 to December 1999. Risk categorization was on the basis of the NNIS system. P <.05 was set for statistically significant difference between groups. Data were analyzed using the Epi-Info software (version 6.04, CDC, Atlanta, Ga). RESULTS: Overall SWI rates for the NNIS risk categories 0, 1, 2, and 3 were 1.9%, 3.7%, 6.7%, and 5.1%, respectively. SWI rate in 0 risk category decreased from 3% in 1997 to 1.1% in 1999 (P =.06). Multivariate analysis showed that SWI rates were higher after mastectomy (odds ratio [OR] 4.28, 95% confidence interval [CI] 1.8-10), hernia repair (OR 3.28, 95% CI 1.6-6.7), gastrointestinal resection (OR 2.2, 95% CI 0.88-5.9), skin procedures (OR 1.97, 95% CI 0.89-4.3), appendectomy OR 0.57, 95% CI 0.20-1.60, and miscellaneous procedures (OR 3.6, 95% CI 1.6-7.7), as compared with cholecystectomy. Other risk factors were contaminated type of operation (OR 2.6, 95% CI 1.2-5.5), and duration of operation exceeding the NNIS standard of "T" hours (OR 2.6, 95% CI 1.7-4). CONCLUSION: The SWI rates at the Aga Khan University Hospital are higher than the NNIS standards. There was a downward trend in the SWI rates during the surveillance period. A decrease in the duration of surgical procedures could further reduce the risk.

Cross Infection↗
PubMed2002

Cystic fibrosis presenting as acute pancreatitis and obstructive azoospermia in a young adult male with a novel mutation in the CFTR gene.

Cystic fibrosis is rare in the Asian population, and is often associated with consanguinity and rare genotypes. We report on a 23-year-old Asian man from a consanguineous pedigree referred to the regional cystic fibrosis unit after a diagnosis of congenital bilateral absence of the vas deferens during investigations for infertility. A detailed history revealed several previous episodes of acute pancreatitis. Full diagnostic appraisal showed homozygosity for a novel cystic fibrosis transmembrane conductance regulator (CFTR) gene mutation, but normal sweat test and nasal potential difference studies. An endoscopic retrograde cholangiopancreatogram (ERCP) showed chronic pancreatitis with bulky side branches. The vas deferens and the pancreas appeared exquisitely sensitive to mild CFTR dysfunction. Patients with cystic fibrosis and unexplained upper abdominal pain should be screened for pancreatitis, and consideration should be given to screening patients with idiopathic pancreatitis for mutations in the CFTR gene.

Acute Disease↗
PubMed2002

Cryptorchidism.

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Adult↗