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Mirjana Ziemer

Publications and source records attributed to Mirjana Ziemer.

18 recordsLinked to original sources
PubMed2006

IL-6 and IL-8 release is mediated via multiple signaling pathways after stimulating dendritic cells with lysophospholipids.

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are bioactive lipid mediators, which are known to play major roles in allergic reactions as well as in tumor pathogenesis. Here, the biological activities and signal pathways of these lysophospholipids (LPLs) in dendritic cells (DCs) were characterized further. Flow cytometric and immunoblot analyses indicate that immature as well as mature DCs express the LPL receptors S1P1, S1P3, S1P5, and LPA2, but not S1P2, S1P4, LPA1, or LPA3. Moreover, enzyme-linked immunosorbent assay experiments demonstrate that simultaneous addition of these LPLs to immature DCs in the presence of lipopolysaccharide enhanced the secretion of the inflammatory cytokines interleukin (IL)-6 and IL-8 in maturing DCs. In contrast, no modification of IL-6 or IL-8 release was observed after exposure of mature DCs to LPLs alone. In addition, studies with pertussis toxin and mitogen-activated protein kinase (MAPK) kinase inhibitor PD98059 suggested that Gi proteins and MAPK pathway are involved in these LPL-induced cell responses. Corroborating these findings, we observed that LPLs induce the phosphorylation of extracellular signal-regulated kinase 1/2 in immature DCs but not in mature DCs. Further analyses show that inhibitors of phosholipase D, Rho, and protein kinase C also inhibited the LPL-induced release of IL-6 and IL-8. Therefore, our findings suggest that lipopolysaccharide in DCs uncouples LPL receptors from the signal-transducing machinery during maturation and that exposure of LPLs at early time-points to maturing DCs modifies the proinflammatory capacity of mature DCs.

Cells, Cultured↗
PubMed2006

Epidermotropic metastases from squamous cell carcinoma of the lower female genital tract mimicking primary Bowen's carcinoma.

Epidermotropic metastases from internal malignancies are exceedingly rare. We report a striking example of epidermotropic metastatic carcinoma of the lower female genital tract to the skin of the abdomen and groin. Atypical cells were scattered throughout the epidermis, mimicking Bowen's disease. Focally, islands of atypical squamous cells were also found in the adjacent dermis. Histopathologically, the lesion resembled a primary invasive squamous cell carcinoma. However, the clinical history of a metastatic carcinoma of the genitalia and the clinical presentation with multiple lesions militated against a primary squamous cell carcinoma and favored interpretation of the lesions as epidermotropic metastases.

Bowen's Disease↗
PubMed2006

Treatment of superficial surgical wounds after removal of seborrheic keratoses: a single-blinded randomized-controlled clinical study.

BACKGROUND: For the treatment of superficial surgical wounds, there are a number of options, including topical antibiotic ointments, dressings, and specialized wound care materials, such as hydrocolloid dressings. OBJECTIVE: To evaluate the wound-healing activity of a commercially available hydrocolloid wound dressing (Avery H2460, Avery Dennison, Turnhout, Belgium) in comparison with a control treatment (Fucidine cream with Cutiplast sterile dressing) in superficial wounds after surgical removal of seborrheic keratoses. METHODS: In a single-blinded, randomized, controlled trial, the hydrocolloid wound dressing (Avery H2460) was compared with healing by secondary intention as a control. Sixteen patients between 18 and 80 years of age with seborrheic keratoses were enrolled. Wound healing was evaluated after 7 and 10 days and then daily until complete closure of the wound area. In 7 of 16 patients, biopsies were taken after 14 days of reepithelization. RESULTS: The hydrocolloid wound dressing (Avery H2460) induced a significantly (p<.05) faster healing (median: 8.5 days) in comparison with the control treatment (median: 10 days). The histologic investigations showed no significant differences for the investigated parameters in both groups. CONCLUSION: The faster healing in comparison with the control treatment supports the use of the hydrocolloid wound dressing (Avery H2460) for the treatment of superficial surgical wounds.

Bandages, Hydrocolloid↗
PubMed2006

[Umbilical endometriosis].

A 40-year-old woman with a history of a growing erythematous nodule in the umbilicus was diagnosed histopathologically with endometriosis. This umbilical endometriosis occurred spontaneously without any previous surgical procedures. The lesion was partially removed surgically; she then entered menopause with a spontaneous involution of the residuum during the following months so that further invention was not required. Umbilical endometriosis is very rare with an estimated incidence of 0.5 to 1% of all patients with endometrial ectopia. As the clinical appearance of umbilical endometriosis may share features with a variety of other disorders, we discuss the clinical picture and differential diagnostic considerations in detail.

Adult↗
PubMed2005

Eosinophilic pustular folliculitis in infancy: not a distinctive inflammatory disease of the skin.

In the standard literature of dermatology and dermatopathology "eosinophilic pustular folliculitis in infancy" is presented as a distinctive inflammatory disease of the skin, to wit, a variant of "Ofuji's eosinophilic folliculitis". Assessment critically of the first publication devoted to the subject revealed a potpourri of findings clinical and histopathologic, with no clear criteria being set forth by the authors to enable diagnosis, with precision. Our review of all articles dedicated to the matter of "eosinophilic pustular folliculitis in infancy" shows that criteria for diagnosis are found with great difficulty. The majority of patients reported on had neither "eosinophilic folliculitis," nor did they present themselves clinically in any way similar to what was originally described by Ofuji. The findings histopathologic told of most commonly were dense and diffuse infiltrates in the dermis that contained many eosinophils, sometimes with periadnexal distribution. We think that the findings reported on in several articles suggest that the patients more likely had a variety of different diseases, among them scabies, insect bites, impetigenized nummular dermatitis, and linear IgA-dermatosis. For clarification of terminology, we suggest that "eosinophilic folliculitis" is better defined as a pattern histopathologic than as a distinctive disease entity. It may be encountered in a variety of conditions (eg, Ofuji's disease, arthropod bites, scabies, or dermatophytosis). In sum, no clear criteria have been established for diagnosis of "eosinophilic pustular folliculitis of infancy" and there is no convincing evidence, at present, that "eosinophilic pustular folliculitis of infancy" qualifies as a distinctive inflammatory disease of the skin.

Child↗
PubMed2005

'Specific' cutaneous infiltrate of B-cell chronic lymphocytic leukemia at the site of a florid herpes simplex infection.

BACKGROUND: Specific cutaneous infiltrates in patients with leukemia generally carry a grim prognosis. However, non-neoplastic skin diseases may be associated with recruitment of normal and neoplastic leukocytes circulating in the peripheral blood. In those instances, neoplastic cells may be detected in skin lesions without an adverse effect on prognosis. METHODS: In a patient with B-cell chronic lymphocytic leukemia, a specific infiltrate developed at the site of a florid herpes simplex infection. Clinically, the lesion presented itself as an ulcerated tumor. RESULTS: Histopathologically, the lesion was characterized by a dense, diffuse infiltrate of small hyperchromatic lymphocytes throughout the entire dermis. Lymphocytes showed an aberrant CD20(+)/CD43(+)/CD5(+) phenotype of neoplastic B cells, and monoclonal rearrangement of immunoglobulin gamma genes could be demonstrated by polymerase chain reaction. Although criteria for leukemia cutis were fulfilled, the patient did well. CONCLUSIONS: The cutaneous infiltrate of neoplastic cells seemed to be part of a physiologic response to the antigenic stimulus, rather than indicating an exacerbation of leukemia. Ziemer M, Bornkessel A, Hahnfeld S, Weyers W. 'Specific' cutaneous infiltrate of B-cell chronic lymphocytic leukemia at the site of a florid herpes simplex infection.

Aged↗
PubMed2004

Treatment of extensive chronic cutaneous graft-versus-host disease in an infant with topical pimecrolimus.

We report a case of generalized cutaneous chronic graft-versus-host disease in a 17-month-old infant. Topical treatment with the novel ascomycin substance pimecrolimus once daily for 4 weeks led to a near complete clearance of skin lesions. Importantly, this excellent clinical response was obtained without additional use of systemic immunosuppressive treatment.

Administration, Topical↗
PubMed2004

[Relapsing polychondritis as a rare different diagnosis of erysipelas].

A 76-year old patient with painful erythematous swelling of the right ear was initially treated with antibiotics under the suspected diagnosis of erysipelas. Her failure to respond and a history of previous laryngeal and nasal swelling suggested the possibility of relapsing polychondritis. High dose prednisolone therapy produced marked improvement. Relapsing polychondritis should be considered as a rare, but important differential diagnostic consideration for erysipelas.

Aged↗
PubMed2004

Combined 5-fluorouracil and Er:YAG laser treatment in a case of recurrent giant keratoacanthoma of the lower leg.

BACKGROUND: Keratoacanthomas are fast-growing squamous tumors, which usually show spontaneous regression. The development of giant variants with an aggressive behavior has been described. Although surgical excision remains the treatment of choice for very large keratoacanthomas, other therapeutic options including laser surgery and topical chemotherapy may be superior in special situations. OBJECTIVE: The objective was to evaluate the efficacy of Er:YAG laser surgery combined with topical 5-fluorouracil treatment in a case of recurrent giant keratoacanthoma. METHODS: A 64-year-old woman presented for evaluation and treatment of recurrent tumors in her face and extremities. Despite repeated invasive surgical removal of these lesions, recurrence of fast-growing giant keratoacanthomas developed in the pretibial region of her left lower leg. Owing to recurrence after conventional surgery and the tumor size, a novel treatment method using ablative Er:YAG laser combined with topical 5-fluorouracil was performed. RESULTS: After four treatments with excellent patient compliance, histologic analysis of punch biopsies revealed tumor-free ulcerations. Complete epithelization was obtained after 9 weeks. Six months after the treatment, no recurrence was observed. CONCLUSION: The combined use of ablative Er:YAG laser and topical 5-fluorouracil chemotherapy may be considered as an effective treatment option in cases of giant keratoacanthoma when conventional surgery is not indicated.

Administration, Cutaneous↗
PubMedDate not supplied

Necrolytic migratory erythema with myelodysplastic syndrome without glucagonoma.

Necrolytic migratory erythema is a cutaneous paraneoplastic manifestation, which is usually associated with a glucagon-secreting pancreatic tumor. However, it also may occur in other circumstances in which serum glucagon is elevated, as in hepatic cirrhosis. Rarely, necrolytic migratory erythema is reported in association with a jejunal and rectal adenocarcinoma or villous atrophy of the small intestine without any evidence for increased serum glucagon levels. In this context we report the case of an 85-year-old male with myelodysplastic syndrome who developed typical necrolytic migratory erythema without glucagonoma syndrome or evidence for other pancreatic or liver disease. We suggest that, in addition to the diseases listed, myelodysplastic syndrome might be able to cause necrolytic migratory erythema.

Aged↗
PubMedDate not supplied

Necrobiotic xanthogranuloma-rapid progression under treatment with melphalan.

Necrobiotic xanthogranuloma is a systemic disease associated in most cases with monoclonal paraproteinaemia. Although the causative role of the paraproteinaemia is supposed, the pathogenesis of this disease remains unknown. We report a 65-year-old woman with a 28-year history of disseminated indurated plaques involving her face, trunk and extremities. Since 1994 an IgG kappa paraproteinaemia was present. Current biopsies showed typical histological features of necrobiotic xanthogranuloma. We treated this extraordinary widespread condition with melphalan and prednisolone. Although the serum levels of IgG kappa light chains and gamma globulins decreased, the cutaneous plaques extended rapidly in size and number, which casts doubt on the causative significance of the paraproteinaemia in the pathogenesis of necrobiotic xanthogranuloma. The paraproteinaemia in patients with necrobiotic xanthogranuloma may rather reflect a secondary phenomenon than the originating cause. Pathogenetic and therapeutical concepts based thereon that have been proposed so far should be critically reconsidered.

Aged↗