Biomedical subjects

Min Zhao

Publications and source records attributed to Min Zhao.

118 records · Page 7Linked to original sources

PubMed2002

[Correlation of angiogenesis with expression of vascular endothelial growth factor and its receptors in lung carcinoma].

OBJECTIVE: In order to search new therapeutic strategies, study the relationship between angiogenesis and vascular endothelial growth factor and its two receptors in lung carcinoma. METHODS: The study consisted of 49 patients with lung carcinoma treated with curative surgery. Immunohistochemistry on paraffin sections was performed with anti-human factor VIII antibody to study the microvascular density (MVD), and with antibodies to VEGF, Flt1, and KDR to investigate the expression of these three proteins in different cellular compartments. The survival time was compared between low MVD and high MVD groups by the Kaplan-Meier method. RESULTS: (1) Expression of VEGF and its two receptors in tumor cells, stromal fibroblasts and endothelial cells was demonstrated. (2) There was no significant difference between low MVD and high MVD groups in the clinical data, including TNM stage, clinical phase, pathologic type, and tumor cell differentiation (P > 0.05). Survival analysis showed that the high MVD group was associated with a high risk of death. (3) There was no significant difference between low MVD and high MVD groups in VEGF expression in tumor cells, but in endothelial cells both Flt1 and KDR were correlated with high microvessel count. (4) The high expression of VEGF in tumor cells was correlated with that in stromal fibroblasts. The level of expression in both cells was consistent. (5) In both tumor cells and endothelial cells, high co-expression of VEGF and KDR was consistent, but that of VEGF and Flt1 showed inconsistency. CONCLUSIONS: (1) Not or rarely affected by clinical factors, MVD is a good and independent prognostic factor for patients with lung carcinoma. (2) The angiogenesis is induced not only by VEGF itself, but via VEGF receptors too. VEGF and its receptors thus appear to be new therapeutic targets for lung carcinoma. (3) VEGF appears to be produced by both tumor cells and stromal fibroblasts. (4) The result suggests that tumor expansion and angiogenesis are mainly induced by an autocrine pathway and a paracrine loop of VEGF via KDR.

Adult↗

PubMed2002

[CCA of water beetles' distribution and environmental factors in lentic samples of north Changbai Mountain].

The relationship between 28 species water beetles in 12 lentic samples and environmental factors of North Chang-bai Mountain was studied by Cononical Correspondence Analysis (CCA). The results showed that degree of underwater humus and altitude are the major factors correlated with beetles distribution, and the correlation coefficients of environmental factors and axes of CCA were 0.8371 and 0.7206 respectively, while water temperature and plant density also had certain effects. Under the influence of environmental factors, the water beetles' populations were different in different habitat. Coelambus impressopunctatus, Colymbetes magnus, Helophorus browni, Haliplus spp. distributed in deep water pool. Water temperature was not important for those beetles. Ilybius sp. and Limnebius glabriventris correlated with altitude and humus.

Animals↗

PubMed2002

[A study of the pour blood and the oxygen metabolic in tissue for extra-artery fascia flap].

OBJECTIVE: The aim of the work was to study the change for blood pour into the artery fascial flap and oxygen metabolic in tissue that knew the process of the flap. METHODS: We do the artery fascial flap in the rabbit, then measured the skin capillary blood flow and the content of the MDA. RESULTS: The content of the MDA rise when the blood flow descends in the flap, but it lives well. CONCLUSIONS: That the content of the MDA rise as time the blood flow descend in the flap is a gradual process of the whole flap from tip to end, the flap will not appear necrosis until the MDA reach a rather degree.

Animals↗

PubMed2002

[Subcutaneous injection of plasmid VEGF gene: a method of gene therapy to enhance the viability of random skin flap].

OBJECTIVE: Gene therapy has been becoming one of the most attractive medical areas. But the using of gene therapy in plastic surgery is relatively scarce. Our purpose was to investigate the effect of naked plasmid encoding Vascular Endothelial Growth Factor on the viability of the random skin flap by directly injected subcutaneously. METHODS: 30 female Sprague-Dawley rats randomly divided into three groups. A random dorsal skin flap of 3 cm x 9 cm was elevated in each of the rats. And 1 ml double-distilled water solution was injected subcutaneously, which was only water in group 1 during the operation, 200 micrograms VEGF cDNA plasmid in group 2 during the operation, 200 micrograms pcDNA3.1/zeo(+)--VEGF in group 3, 24 hours before the operation, respectively. 7 days after the operation, all the animals were sacrificed by overdose anesthetic. The survival tissue was measured with planimetry. Two samples were harvested from each group for pathological check and immunohistochemical test. RESULTS: Immunohistochemical staining demonstrated that there was human VEGF deposited around the capillary in the flaps treated with VEGF gene. The flaps treated with VEGF gene had a larger percentage of survival skin (group 1 = 47% +/- 5.4%, group 2 = 65.4% +/- 6.3%, group 3 = 72.3% +/- 8.5%, P < 0.05). CONCLUSION: VEGF gene directly injected into subcutaneous can express VEGF. It makes the gene therapy simple and practical and will be promising future in the tissue transplantation.

Animals↗

PubMed2002

[Physical and psychological changes in patients with traumatic deformities during the treatment of tissue expansion].

OBJECTIVE: This study was to investigate the somatic symptoms and sychological specialties in trauma-deformed patients during tissue expansion. METHODS: Using our own questionnaires, fifty-seven patients were solicited to answer the questionnaires in three different treatment periods. RESULTS: It was found that local distension, pain and tickle were the common symptoms during expansion, while distension was most specific (P < 0.01). Pain and tickle also happened before implanting and after taking out of the expander. The patients often had high expectation toward tissue expansion. They felt nervous and anxious (P < 0.01) during expansion. Their emotion and healthy self-evaluation changed obviously in different treatment periods (P < 0.01). CONCLUSION: Local distension is the most specific symptom during tissue expansion. Nervousness and anxiety are common in patients at that time. Their emotion change from depression to happy at first, and then from happy to slight depression.

Adolescent↗

PubMed2002

[Analysis of genetic instabilities on chromosome loci 3, 6, 11, and 18 in human cervical carcinoma].

BACKGROUND & OBJECTIVE: The infection with human papillomavirus and cellular genetic alteration appear to be necessary etiological factors for cervical carcinoma. This study was designed to investigate the genetic instability in cervical carcinoma tissues and provide evidence for discovering new tumor suppressor genes and screening diagnostic molecular marker of cervical carcinoma. METHODS: Fifty primary cervical carcinoma specimens from high-incidence area was examined through loss of heterozygosity(LOH) and microsatellite instability(MI) analysis with eight polymorphic microsatellite markers on chromosome 3, 6, 11, and 18. RESULTS: LOH was observed at one or more loci in 33 cases(66%). The highest frequency of the allelic loss was found in D18s474 (18q21, 40.5%). LOH was detected on other sites including D3s1478 (3p21.3-21.2, 31.7%), D3s1766 (18q21.32, 15.0%), D6s260 (6p23, 23.3%), D11s925 (11q22-23, 17.9%), D18s35 (18q21.1-21.31, 8.7%), D18s64 (18q21.32, 16.7%), and D18s68 (18q22.1, 27.3%). MI was detected in 4 cases(8%). CONCLUSION: Different percentages LOH on specific chromosomal regions were found and MI was very infrequent in cervical carcinoma tissues. The results of the current study strongly suggest that the putative suppressor gene(s) likely to be located on specific chromosome region 3p or 18q involve in cervical tumorigenesis.

Adenocarcinoma↗

PubMed2002

[Analysis of 5459 cleft lip and palate cases].

OBJECTIVE: To analyse the constitution of 5459 cleft lip and palate cases and to review the development of surgical treatment to them. METHODS: The data of the 5459 cleft lip and palate cases were collected and classified. The ratio of sex and disease pattern were calculated and analyzed statistically. Also, by means of classification of the disease pattern, the operation methods and the operation time, the change of the surgical methods and the first operation age were analyzed. RESULTS: In the cleft lip and palate cases, the sex ratio is different. There were obvious differences in the first operative ages in different periods. The main repair methods for cleft lip and palate changed with time. CONCLUSION: The male is more than the female in the cleft lip and palate patients. The average first operation age has been decreased and the main repair methods have changed since 1980.

China↗

PubMed2002

[Effects of exogenous IL-10 on IL-6 and ICAM-1 expression in inflammatory gingival tissue].

OBJECTIVE: This study aimed at investigating effects of exogenous interleukine-10 (IL-10) on IL-6 and intercellular adhesion molecular (ICAM-1) expression in inflamed gingival tissue. METHODS: The expression of IL-6 and ICAM-1 was examined using immunohistochemical techniques. Inflammatory gingival tissue treated with IL-10 was taken as the experimental group and the same patient's inflammatory gingival tissue without treatment of IL-10 was included into the control group. RESULTS: IL-6 expression was found mainly in monocytes, macrophages, lymphocytes, endotheliocytes and fibroblasts. The expression of ICAM-1 was found mainly in epithelial cells, monocot-macrophages, lymphocytes, endotheliocytes and fibroblasts. The immunohistochemical optical density (IOD) of the expression of IL-6 and ICAM-1 was detected by using Image-Proplus software, and the results showed that the expression in the experimental group differed significantly from that in the control group. CONCLUSION: The exogenous IL-10 may down-regulate IL-6 and ICAM-1 expression in inflammatory gingival tissue.

Adult↗

PubMed2002

[Clinical studies on the short-course and efficient treatment of mumps meningitis].

OBJECTIVE: To investigate the short-course and efficient way to treat mumps meningitis. METHODS: Totally 155 cases of mumps meningitis treated by intrathecal injection with dexamethasone only once were enrolled as experimental group and 55 similar cases treated with the common therapy as control. RESULTS: The time for recoveries of temperature, headache, the pathologic reflexes and the total time of treatment were 32 hours, 15 hours, 12 hours and 3.1 days, respectively, while those of the control group were 58 hours, 24 hours, 32 hours, 6.5 days respectively. There was statistical difference between the two groups (P<0.01 or 0.05). CONCLUSIONS: Intrathecal injection with dexamethasone only once is efficient and safe in the treatment of mumps meningitis.

Anti-Inflammatory Agents↗

PubMedDate not supplied

Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids.

We investigated the effect of cannabinoids on potassium chloride (K+)- and ischemia-induced [3H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [3H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K+ -induced [3H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [3H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.

Animals↗