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Biomedical subjects

Min Zhao

Publications and source records attributed to Min Zhao.

At least 37 records · Page 2Linked to original sources
PubMed2006

Prognostic factors and survival of stage IB-IIA cervical cancer.

OBJECTIVE: This study investigated the prognostic significance of age at diagnosis, stage, tumor subtype, pelvic lymph node metastasis (PLNM), lymph-vascular space involvement (LVSI), presence or absence of deep cervical stromal invasion (DCSI) in stage IB-IIA cervical cancer patients. It also investigated the inter-relationship among these factors. METHODS: 152 patients treated with radical hysterectomy plus pelvic lymphadenectomy were followed up for a median of 49 months and were evaluated retrospectively. RESULTS: The 5-year overall survival rate was 84.8%. The distribution of age at diagnosis is of bimodal shape, peaking at 42 and 68 years, respectively. Tumor subtype, PLNM, DCSI, and LVSI were found to be significant prognostic factors individually. After multivariate analysis, only tumor subtype and PLNM were found to be independent, significant prognostic factors for survival. The prognostic importance of LVSI appeared to be eclipsed by the presence of PLNM. DCSI was statistically related with FIGO stage, LVSI and PLNM. CONCLUSION: Tumor subtype and PLNM are the two most important independent prognostic factors for stages IB-IIA cervical cancer. Some prognostic factors are inter-related and may reflect different facets of tumor progression.

Adolescent↗
PubMed2006

A modified procedure for velopharyngeal sphincteroplasty in primary cleft palate repair and secondary velopharyngeal incompetence treatment and its preliminary results.

During cleft repair, velopharyngeal sphincter reconstruction is still a challenge to plastic surgeons. To improve the surgical treatment for cleft palate and secondary velopharyngeal incompetence (VPI), a carefully designed modified procedure for primary palatoplasty and secondary VPI was presented. Fifty-six patients (48 for primary cleft palate repair and eight for secondary VPI of previously repaired clefts) underwent this procedure from 1988 to 2001. The modified procedure is a combination of the tunnelled palatopharyngeus myomucosal flap for dynamic circular reconstruction of the pharyngeal element of the velopharyngeal sphincter and the double-reversing Z-plasty with levator velo palatini muscles reposition in the velar element of the sphincter. The satisfactory velopharyngeal competence (complete velopharyngeal closure and marginal velopharyngeal closure) was achieved in 23 of 25 patients with primary cleft palate repair examined by nasendoscopy and the nasality, speech articulation and intelligibility are also assessed in 25 primary cleft palate repaired patients with 92% satisfactory result (normal speech and speech with mild VPI) in single word test and 88% in continuous speech evaluation. Based on our experience, we believe that this modified procedure is a reasonable choice for primary cleft repair and secondary VPI treatment because it is in accord with normal physiology and anatomy of the velopharyngeal sphincter, can lengthen the soft palate, decrease the enlarged velopharynx, augment the posterior pharyngeal wall, and enhance the relationship between the muscles of velopharyngeal sphincter which results in a dynamic neo-sphincter in palatopharyngoplasty. Further study of the procedure is needed. The theoretical basis, operative highlights, velopharyngeal function, advantages and disadvantages of the modified procedure were discussed.

Adolescent↗
PubMed2006

Randomized double-blinded and controlled clinical trial on treatment of HIV/AIDS by Zhongyan-4.

OBJECTIVE: To assess the efficacy and safety of Zhongyan-4 (ZY-4, a Chinese herbal preparation worked out according to the therapeutic principle of supplementing qi, nourishing Yin, clearing heat and detoxication) in treating HIV/AIDS patients in the early or middle stage. METHODS: Adopted was randomized double-blinded and placebo-parallel-controlled method, with 72 HIV/AIDS patients randomly divided into the ZY-4 group (36 patients) treated with ZY-4 and the control group (36 patients) treated with placebo. The treatment course was six months. The index of CD(4)(+), CD(8)(+) counts, body weight, clinical symptom scoring were estimated at 4 time points (0, 1, 3 and 6 month in the course), and also the viral load before and after treatment. The whole course of observation was completed in 63 patients, 30 in the ZY-4 group and 33 in the control group. RESULTS: CD(4)(+) count in the ZY-4 group got elevated by 7.70 +/- 150.96/mm(3) on average, while that in the control group lowered by 27.33 +/- 85.28/mm(3). Fifteen out of the 30 patients in the ZY-4 group had their CD(4)(+) count increased, which was evidently much higher than that in the control group (8/33, P < 0.05), suggesting that the efficacy of ZY-4 is superior to that of placebo in elevating CD(4)(+) count. Moreover, ZY-4 showed actions in elevating CD(45)RA(+) and CD(8)(+) count, reducing HIV virus load, improving clinical symptom/sign and increasing body weight of patients. No obvious adverse reaction was found in the clinical trial. CONCLUSION: ZY-4 has an immunity-protective and/or rebuilding function in HIV/AIDS patients in the early and middle stage, and also shows effects in lowering viral load, increasing body weight and improving symptoms and signs to a certain degree.

Acquired Immunodeficiency Syndrome↗
PubMed2006

HIV sexual risk behaviors among injection drug users in Shanghai.

PURPOSE: This study investigated the sexual risk behaviors among injection drug users. (IDUs) in order to inform the development of sexual risk reduction interventions for IDUs. METHODS: A cross-sectional survey of IDUs (n=141) was conducted in an in-patient detoxification treatment center in Shanghai, China, to collect information on demographics; drug use history; sexual risk behavior; HIV/AIDS knowledge, attitudes, and other psychosocial variables; and HIV, HBV, and HCV seroprevalence. Factors associated with HIV sexual risk behaviors and HBV and/or HCV infection were analyzed. RESULTS: Sexual risk behaviors among IDUs were common: the majority (77%) of the participants had not used a condon consistently in the previous 3 months, 25.5% had multiple partners, 48.2% had IDU partners, and 75.9% did not know their partner's HIV status. IDUs who were married (OR=4.83, p<0.05) or did not intent to use condoms in the future (OR=0.21, p<0.05) were more likely to have unprotected sex. The prevalence of HBV and HCV infection was 31.9% and 51.8%, respectively, but no one tested positive for HIV.IDUs with an injection history of 3 years or more (OR=5.86, p<0.05) and with an overdose history (OR=3.21, p<0.05) were more likely to be infected with HBV and/or HCV. CONCLUSIONS: Sexual risk behaviors among IDUs in Shanghai are common, and many IDUs are vulnerable for transmission of disease. Prevention efforts with IDUs should address sexual risk behaviors in addition to needle-sharing behaviors.

Adult↗
PubMed2006

Subpopulation of dorsal horn neurons displays enhanced N-methyl-D-aspartate receptor function after chronic morphine exposure.

BACKGROUND: Morphine tolerance may be attributed to enhancement of glutamatergic neurotransmission, in particular to increased function of the N-methyl-D-aspartate (NMDA) receptor. The cellular mechanisms responsible for these changes remain poorly defined. The authors identified and characterized a specific subpopulation of dorsal horn neurons, displaying NMDA receptor plasticity in response to chronic morphine administration. METHODS: The authors undertook current clamped and voltage clamped recordings of NMDA receptor-mediated responses from cultured rat dorsal horn neurons that were untreated or treated for 7 days with 1 or 100 microm morphine. RESULTS: Smaller (capacitance < or = 22 pF), tonic firing neurons showed a significantly enhanced NMDA receptor-mediated peak current after prolonged morphine treatment, whereas larger and phasic firing neurons showed no enhancement. With high-concentration but not low-concentration morphine treatment, Mg2+ blockade of NMDA receptors at resting membrane potentials was reduced. Furthermore, the chronic opioid-induced increase in NMDA current was attenuated by pretreatment with either a mu-opioid receptor inhibitor (naloxone) or an NMDA receptor inhibitor (2-amino-5-phosphonovalerate) (low-concentration > high-concentration morphine). CONCLUSIONS: In an electrophysiologically defined subpopulation of dorsal horn neurons, enhanced NMDA receptor function after chronic morphine exposure was shown to be mechanistically dependent on morphine concentration and sensitive to both NMDA and mu-opioid receptor antagonism. Therefore, these changes observed in this population of sensory spinal neurons can be used to study the development and prevention of opioid tolerance described in multiple laboratory and clinical reports.

2-Amino-5-phosphonovalerate↗
PubMed2006

Clinical and pathological study of lichen-planus-like keratosis in China.

Lichen-planus-like keratosis is usually diagnosed pathologically; rarely, a definitive diagnosis can be made grossly in the clinic. Only a few cases of lichen-planus-like keratosis have been reported in China. The purpose of this study was to investigate the clinical and pathological features of lichen-planus-like keratosis in China. Fifty cases of lichen-planus-like keratosis patients diagnosed pathologically during a 5-year period in our clinic were analyzed. Clinical features were recorded. Sectioned specimens were subjected to hematoxylin and eosin staining to observe pathological changes. Results showed that there were 34 males and 16 females (ratio 2:1) with an average age of 61.2 years. Most of the lesions were single papules or plaques with rough surfaces. They were distributed on the face, larger than 1 cm, and dark red to brown in color. Only one case (2%) was considered to be lichen-planus-like keratosis clinically. By hematoxylin and eosin staining, solar lentigo and solar elastosis could be found in 68% and 32% of lichen-planus-like keratosis lesions, respectively. Eosinophil (42%) and plasma cell (36%) infiltration was also found frequently. Exoerythrocytes could be detected in 50% of the cases. Lichen-planus-like keratosis is not uncommon in clinical practice in China, the diagnosis of lichen-planus-like keratosis should be made by a combination of clinical manifestations and pathological changes. It is better to classify lichen-planus-like keratosis as a benign skin tumor. More attention should be paid to lichen-planus-like keratosis in China.

Aged↗
PubMed2006

[The usage of inferior turbinate mucoperiosteal flap for repairing cleft palate].

OBJECTIVE: To evaluate a technique for closing the cleft of nasal aspect at the junction of the hard and soft palate and ensuring the wound healing well during the procedures of repairing broad cleft palate. METHODS: A inferior turbinate mucoperiosteal flap based posterior is designed and transposed to the junction of the hard and soft palate for decreasing the tension effectively and closing the cleft easily. RESULTS: All of the patients showed wound healing well and no distinct scars or palatal fistula. CONCLUSIONS: It is a simple and effective technique to decrease the tension and close the cleft of nasal aspect at the junction of the hard and soft palate with inferior turbinate mucoperiosteal flap.

Child, Preschool↗
PubMed2006

[Antifungal susceptibility testing of commensal and pathogenic clinical isolates of oral Candida].

PURPOSE: To evaluate and compare the sensitivity and resistance of commensal and pathogenic oral Candida to antifungal agents. METHODS: YBC Test Kit was used to identify species of commensal and pathogenic oral Candida; The in vitro susceptibilities of oral Candida to 5-flucytosine (5-FC), itraconazole (ITR) and fluconazole (FLU) were determined by NCCLS M27-A2 method. RESULTS: All strains were sensitive to 5-flucytosine, drug resistant strains to fluconazole and itraconazole were found both in commensal and pathogenic oral Candida spp; The proportion of no-C. albicans and antifungal drug resistant strains isolated from head and neck cancer patients treated with radiotherapy or chemotherapy were higher than oral commensal Candida and oral Candidiasis. CONCLUSIONS: The no-C. albicans and drug resistant strains to fluconazole and itraconazole can be found both in commensal and pathogenic oral Candida spp now.

Antifungal Agents↗
PubMed2006

[Antifugal susceptibility testing and antifugal traditional Chinese medicines screening of oral Candida isolated from head and neck cancer patients treated with radiotherapy or chemotherapy].

OBJECTIVE: To evaluate the sensitivity and resistance of pathogenic oral Candida spp. isolated from head and neck cancer patients treated with radiotherapy or chemotherapy to antifungal agents. To screen antifugal agents from Chinese traditional and herbal drugs by NCCLS M27-A2 method. METHODS: Using YBC Test Kit to identify 20 clinical oral Candida isolated from head and neck cancer patients treated with radiotherapy or chemotherapy. The in vitro susceptibilities of 20 oral Candida spp. to 5-flucytosine (5-FC), itraconazole (ITR), fluconazole (FLU), the extracts of 6 Chinese traditional and herbal drugs (caltrop, honeysuckle flower, dandelion, green tea, pine bark, red trefoil) and utility componets of 7 Chinese traditional and herbal drugs (sophorcarpidine, aloperine, archin, glycyrrhizic acid, glycosides of white peony root, glycosides of baikal skullcap root, hydrochloric berberine) were determined by NCCLS M27-A2 method. RESULTS: The proportion of no-C. albicans in all Candida spp. were 25%. All strains were sensitive to 5-flucytosine, 25% stains were resistant to fluconazole and 40% stains were resistant to itraconazole. In all agents from Chinese traditional and herbal drugs, glycosides of white peony root and hydrochloric berberine (C20H18CINO4) exhibited antifungal activity, especially to C. glabrates. CONCLUSION: The proportion of no-C. albicans in all oral Candida spp. isolated from head and neck cancer patients treated with radiotherapy or chemotherapy was pretty high. NCCLS M27-A2 micro-dilution method is a reliable and reproducible method and can be used to screen antifugal agents from Chinese traditional and herbal drugs.

Antifungal Agents↗
PubMed2006

[Lateral radiographic comparison for velar movement between palatoplasty with velopharyngeal muscular reconstruction and modified Von Langenbeck's procedure].

OBJECTIVE: To verify the effect of palatoplasty with or without velopharyngeal muscular reconstruction on the velar movement through the lateral radiography. METHODS: From October 1988 to October 2000, 62 patients with cleft palate and velopharyngeal insufficiency were treated. Of them, 32 were repaired by velopharyngeal muscular reconstruction (group A) and 30 by modified von Langenbeck's procedure (group B). The lateral radiographs and cephalometric analysis were taken. The velopharyngeal closure, velar extensibility, the angle changes of velar elevation, the distance changes of velar levator eminence based on anatomy (LEA) to velopharyngeal closure line (VCL), the varieties in LEA, the comparison of LEA and velar levator eminence based on phonation (LEP), the distance comparison between posterior nasal spine (PNS) to LEA and LEP through soft palate line (SPL) were measured. RESULTS: During the phonation, group A was significantly greater than group B in the velopharyngeal closure rate (P<0.01), the velar extensibility (P<0.05) and the location comparison between LEA and LEP (P<0.01); group B was significantly greater than group A in velar elevation angle (P<0.05), the varieties in LEA (P<0.05). In velar rest position, the distance of LEA to VCL was greater in B group than in A group(P<0.01). There was significant difference in the distance comparison between LEA and LEP(P<0.05), difference between LEA and LEP (P<0.01) and the distance PNS-SPL-LEA and PNS-SPL-LEP(P<0.05) within group B; contrary to the results within group A (P>0.05). CONCLUSION: The velopharyngeal muscular reconstruction in palatoplasty can result in a near normalization of anatomic measurement of velar levator muscles and improve the velar function and velopharyngeal competence. Repositioning of velar muscles in a more anatomic correct, transverse position is more important to improve the velar length and accordant velar movement with velopharyngeal muscles in functional palatoplasty. The velar angle change and velar elevation during phonation are not determinative factors for velopharyngeal competence.

Adolescent↗
PubMed2006

[Inhibitory effect of bladder cancer related protein gene on HeLa cell proliferation].

BACKGROUND & OBJECTIVE: Bladder cancer related protein (BLCAP) gene was found downregulated most in primary cervical cancer tissues using oncogene/tumor suppressor gene microarray screening in our previous study, therefore this study was to explore the possible correlation between BLCAP gene and cervical cancer. METHODS: BLCAP expression was investigated in 54 cervical cancer and 25 normal tissues by reverse transcription polymerase chain reaction (RT-PCR). Full length of BLCAP cDNA was cloned into pLXSN expression vector and stably transfected into cervical cancer cell line HeLa cells. Cell proliferation, colony formation ability and apoptosis were determined by cell number counting, colony formation and DNA ladder assay. Nude mice were used to study the anti-tumor effect of BLCAP gene in vivo. RESULTS: BLCAP gene expression was significantly down-regulated or even not observed in human cervical cancer tissues compared to the normal ones. The cell doubling time of HeLa cells transfected with BLCAP was significantly elevated to 69.4 hrs (P<0.05), compared to that of the parental cells (27.5 hrs) or cells transfected with empty vectors (30.2 hrs). Moreover, in comparison with control cells, the colony formation efficiency of cells transfected with BLCAP gene was significantly lower (t=5.98, P<0.01). BLCAP expression also sensitized Hela cells to apoptosis induced by serum deprivation. In vivo, smaller size of tumors were formed in mice after the injection of cells transfected with BLCAP for 30 days compared to those injected with parental cells or cells transfected with empty vector (tumor wet weights were 1.015 g, 1.612 g, and 1.530 g, respectively, P<0.05). Furthermore, under pathological examinations, tumor tissues formed by cells transfected with BLCAP gene displayed less invasive potential with integral vascular fibrous capsules; muscle or adipocyte tissue invasion was not observed. In comparison, necropsy revealed that the tumors formed from the control cells were attached to the underlying muscles; histologically, the neoplastic cells were locally invasive and associated with fibrous connective tissues. These cells exhibited severer cytoplasmic and nuclear pleomorphism compared to cells transfected with BLCAP. CONCLUSION: BLCAP gene is down-regulated in cervical carcinoma tissues and could suppress tumorigenic ability and growth of HeLa cells, thus it may be a potential suppressor candidate gene of cervical carcinoma.

Animals↗
PubMed2006

[Prevalence of bacterial vaginosis in married women of reproductive age in the rural area of Shandong province and its risk factors].

OBJECTIVE: To explore the prevalence of bacterial vaginosis of married women of reproductive age in the rural area of Shandong province and its affecting factors, with an aim to provide references for setting up preventing measures for this disease. METHODS: A stratified, population-based, cluster sampling method was employed. The epidemiological investigation on bacterial vaginosis of married women of reproductive age was carried out at four sampling fields. RESULTS: The prevalence of bacterial vaginosis was 6.64%. The main risk factors included: lacked of personal bathtub and towel; did not use sterilized paper on menstrual phase; had no knowledge that gonorrhea, syphilis, AIDS, and trichomonal vaginitis can be transmitted through sexual intercourse. CONCLUSION: The prevalence of bacterial vaginosis in married women of reproductive age in Shandong rural area is high, which correlates with the lower education level, limited health knowledge, and unhealthy behavior.

Adult↗
PubMed2006

[Prognostic factor analysis of 77 old patients with acute myelogenous leukemia].

BACKGROUND & OBJECTIVE: The manifestations of old acute myelogenous (AML) patients have their special biological and clinical characteristics, with lower response rate to therapy and shorter survival time. This study was to investigate the prognostic factors of elderly patients with AML retrospectively. METHODS: 77 patients aged> or =60 years with AML from 1994 to 2005 were admitted to our study and all the possible prognostic factors were analyzed with Kaplan-Meier survival analysis. The significant factors were further analyzed by Cox proportional hazard model analysis. RESULTS: Seventy-two patients were evaluated. The patients aged 60-70 (median survival time was 350 days) had significantly longer survival time than those aged more than 70 (median survival time is 60 days)(P<0.001), which their CR ratios were 71.4% and 29.4% (P=0.001). The patients with performance status 0 or 1 (median survival time was 402 days) had significantly longer survival time than those with performance status 2, 3 or 4 (median survival time was 31 days)(P<0.001), which their CR ratios were 75% and 15% (P<0.001). The patients with primary AML (median survival time was 98 days) had significantly longer survival time than those with secondary AML (median survival time was 32 days)(P=0.007), which their CR ratios were 50% and 0% (P=0.023). The patients treated with sub-standard dosage of anthracycline (median survival time was 293 days) had significantly longer survival time than those treated with reduced dosage of anthracycline (median survival time was 35 days)(P=0.006), which their CR ratios were 63.6% and 33.3% (P=0.02). The patients with bone marrow blast cell ratio< or =50% (median survival time was 98 days ) had significantly longer survival time than those with bone marrow blast cell ratio >50% (median survival time was 55 days)(P=0.006). The patients with favorable karyotype (median survival time was 293 days) had significantly longer survival time than those with unfavorable or normal karyotype (median survival time was 31 days)(P=0.005). The patients without CD34 expression (median survival time was 201 days) had significantly longer survival time than those with CD34 expression(median survival time was 36 days)(P<0.001). The patients with the peripheral blood white blood cell count (PBWBC)>10x10(9)/L (50%) had significantly higher CR ratio than those with PBWBC< or =10x10(9)/L (25%)(P=0.043). The patients received chemotherapy (50%) had significantly higher CR ratio than those received supportive therapy (0%)(P=0.001). In the stepwise COX proportional hazard regression model, all the seven factors related to OS remained independent and significant. CONCLUSIONS: Factors, including age >70, PS 2 to 4, percentage of blasts in bone marrow >50%, secondary AML, unfavorable karyotype, expression of CD34, lower dosage.

Age Factors↗
PubMed2006

[Study on the genetic polymorphisms of human glutathione-S-transferase A1 in Hakka population in South China].

OBJECTIVE: Glutathione-S-transferase A1 (GSTA1) is one of the major phase II detoxification enzymes in the cytosol, which genetic polymorphisms distribution is different in different ethnic, national and regional population. Up to now, GSTA1 genetic polymorphisms has been rarely reported in China. The purpose of the study was to investigate the distribution of genetic polymorphisms of human GSTA1 in Hakka population in South China. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were used to identify the genotypes of GSTA1 gene and the data were analyzed with SPSS10.0 software. RESULTS: The GSTA1 genetic polymorphisms were detected in 480 samples. The frequency of GSTA1 * A/ * A,GSTA1 * A/ * B and GSTA1 * B/ * B were 77.1%, 21.7% and 1.2% respectively. And the GSTA1 genetic polymorphism distribution was in accordance with the Hardy-Weinberg equilibrium rule. There were no difference in the GSTA1 genetic polymorphisms among the different groups of age or gender. Logistic regression analysis showed that there were no association between the GSTA1 genetic polymorphism and family history of hypertension, coronary heart disease, stroke, lung cancer and nasopharyngeal cancer, et al. CONCLUSION: The GSTA1 gene existed polymorphism among Hakka in South China.

Adolescent↗
PubMed2006

[The effects of hematopoietic stem cells on mice vascular endothelial cells of diabetic retinopathy].

OBJECTIVE: The purpose of the study was to determine whether hematopoietic stem cell (HSCs) mobilization can regulate early diabetic retinopathy in mice. METHODS: Mice were divided into four groups: control group, normal mice HSC-mobilized group, diabetic mice control group and diabetic mice HSC-mobilized group. Murine stem cell growth factor (SCF) and recombined human granulocyte colony stimulating factor (rhG-csf) were administered to the mice with diabetes and without diabetes for continuous 5 days to induce autologous HSCs mobilization, and subcutaneous injection of physiological saline was used as control. The changes associated with autologous HSCs mobilization were characterized using flow cytometry, Immunohistochemistry and semiquantitative RT-PCR. Evans blue quantitative test was used to measure the breakdown level of blood-retina barrier. RESULTS: HSCs were marked by CD34-/low and Sca1+ in this study. The acceleration of endothelial cell regeneration was observed. A decrease of VEGF expression due to autologous stem cell mobilization was found. HSCs could increase the content of VEGFR-2 in mouse retina and significantly downregulated the expression of VEGF and ang-2 in diabetic mice. CONCLUSIONS: The experiment suggest that autologous HSCs mobilization can be approach of therapeutic vascular reconstruction and functional restoration of blood-retina barrier in mice.

Angiopoietin-2↗
PubMed2006

[Study on allergenicity of fresh HAM for type I hypersensitivity].

To investigate whether human amniotic membrane (HAM) preparations have the possibility to type I hypersensitivity and its allergenicity. In systemic active allergic test model, 30 guinea pigs were equally divided into three groups. Each 10 guinea pigs were immunized with fresh HAM homogenate, albumen solution (positive control) and PBS (negative control). After the animals were stimulated with corresponding allergen, observe their reaction till dying or 3 h, then obtain blood samples, to determine blood histamine concentrations using chemical fluorometry and four hemorheologic markers by hemorheology analysis system. The guinea pigs responded to fresh HAM homogenate in almost the same manner as to PBS, and no obvious allergic reaction was observed in the animals except those in positive control group. The blood histamine concentration and four hemorheologic markers showed no significant differences between HAM and PBS (P > 0.05), both were much lower than positive control group (P < 0.01). Fresh HAM won't lead to type I hypersensitivity for lack of allergen performance.

Allergens↗
PubMed2005

[Detection of p53 gene change and serum antibody level in phase II clinical trial of ad p53 gene therapy].

OBJECTIVE: To determine the alteration of p53 gene in tumor tissues and to monitor the immunoresponsiveness to anti adenovirus in patients following treatment of recombinant adenovirus-p53 (Adp53, SBN-1) combined with radiation. METHODS: Tumor tissues were collected from 22 patients with malignant tumors at advanced stage before and after treatment with recombinant adenovirus-p53 (Adp53, SBN-1) combined with radiation. Immunohistochemistry was used to detect the p53 expression. Microdissection, PCR, and denaturation high performance liquid chromatography (DHPLC), and DNA sequencing were used to detect the mutation of p53 gene. The levels of the anti-adenovirus antibody SBN-1, IgG and IgM in the serum were detected by using ELISA. RESULTS: p53 positive staining was shown in the nuclei of tumor cells in 6 of 15 tumor samples showing the increase of stability of protein caused by gene mutation. DHPLC and DNA sequencing showed point mutation in 7 of 22 tumor samples. Six of the 7 cases with p53 gene mutation showed complete and partial remission at the degree > 50%, and the 8 cases without p53 gene mutation only showed partial remission or stability of condition. The IgG positive rate was 50% (8/ 16), showing that 50% of the patients had been infected by adenovirus (mainly the types 3, 7, and 11) before. The IgM positive rate was 6% (1/16), showing that most of the patients had not been infected by adenovirus (mainly the types 3, 7, and 11) at that time. The anti-SBN-1 antibody was negative in all patients before the rAd-53 therapy and became positive since the 4th week after the beginning of treatment and the intensity of positivity increased along with the time. CONCLUSION: An effective gene diagnosis system for detecting the p53 gene alteration has been developed.

Adenoviridae↗