Biomedical subjects

Makoto Nogami

Publications and source records attributed to Makoto Nogami.

3 recordsLinked to original sources

PubMed2003

Analysis of the polymorphic structure of short tandem repeats locus D18S555.

We analyzed the polymorphic structure of the short tandem repeats (STR) (RRGG) locus D18S555 by DNA sequencing, and examined the distribution of alleles in DNA samples from Japanese and Chinese individuals. DNA sequence analysis showed that the alleles at this locus consisted of deletions or insertions of (gaaagaaagaat), (ggaa), (ggaaggaaggag), (ggaaggagggaaggaaggag), (gggaggaa), and (ggga) 5, with each allele having its own DNA sequence. Analysis of 200 unrelated Japanese and 169 unrelated Chinese subjects identified 11 alleles, of which alleles 4, 6, and 13 occurred with a high frequency. There was no difference in allele frequency between the two ethnic groups. These results indicate that the genotyping of D18S555 alleles requires no sequence analysis, and making the locus applicable to forensic identity.

Asian People↗

PubMed2002

Age-associated decrease in 8-hydroxy-2'-deoxyguanosine (8-OHdG) immunoreactivity in the autopsied brain.

The 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a DNA base modified by reactive oxygen species (NOS). The 8-OHdG has been shown to be generated in the brain during ischemia-reperfusion. We performed the immunohistochemical study of 8-OHdG in the brains of autopsied specimens. Age had a statistically significantly negative correlation with 8-OHdG immunoreactivity in glial cells. The 8-OHdG immunoreactivity in glial cells was not increased in the surrounding border of the infarction compared with the non-ischemic areas in the same cases. There was no statistically significant difference in the disease-free areas between infarction and the age-matched control cases. This indicates that 8-OHdG immunoreactivity may not be a sensitive tool to evaluate the infarction injury in human autopsied specimens.

Journal Article↗

PubMed2002

Analysis of the polymorphic structure of the D7S808-short tandem repeat (STR) locus.

We analyzed the polymorphic structure of the short tandem repeat (STR) (AARG) locus D7S808 by DNA sequencing and examined the D7S808 allele distribution in a Japanese population. The sequence analysis confirmed that this locus consists of repeats of the tetranucleotides cttt and cctt, but that the number of repeats of the cctt motif does not vary with the allele, and that this STR polymorphism is due to variation in the number of cttt repeats alone. Although the results in this study suggest that the numbers of repeats range from 7 (allele 7) to 22 (allele 22), alleles 9, 10, 19, and 21 were not observed in the Japanese samples examined. Analysis of DNA samples from 355 unrelated individuals revealed the occurrence of 286 heterozygotes (observed heterozygosity 80.6%). Alleles 15, 14, 16, and 17 had high frequencies of 0.261, 0.192, 0.166, and 0.120, respectively and, together with allele 7 with a slightly high frequency of 0.059, showed a bimodal distribution. In addition, we prepared primers yielding shorter amplification products (232-292 bp) than those (435-480 bp) obtained with the originally reported primers. The newly designed primers can be used for polymerase chain reaction, making this locus extremely useful in forensic science practice.

Journal Article↗