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Biomedical subjects

M Ziegler

Publications and source records attributed to M Ziegler.

At least 415 records · Page 23Linked to original sources
PubMed1977

[Obesity as a metabolic risk factor].

On the basis of the results of clinico-experimental examinations and of animal experiments the importance of obesity as metabolic factor of risk is confirmed and hereby the diabetes mellitus as well as cardiovascular effects are particularly taken into consideration. Conclusions are made relevant to practice and it is particularly referred to the central position of the B-cells for the pathogenesis of diabetes as well as to prophylactic and early therapeutic measures.

Adult↗
PubMed1977

Gilles de la Tourette's syndrome: clinical, genetic, psychologic, and biochemical aspects in 21 selected families.

Eighty-one patients and relatives with Tourette's syndrome, members of 21 selected families, participated in a 1-day clinic. In 12 of the 13 Jewish families and six of the eight non-Jewish families, there were multiple members with motor and vocal tics by observation or history. Males predominated among those with persistent symptoms, but among those with spontaneous clearing, females predominated. Twelve propositi had troublesome sexual and aggressive impulses, differing only quantitatively from normal. No evidence of abnormality was found in plasma dopamine beta hydroxylase, or norepinephrine levels.

Adolescent↗
PubMed1977

Mucopolysaccharide accumulation in cultured skin fibroblasts derived from patients with mucolipidosis IV.

Increased concentrations of total sulfated mucopolysaccharides (MPS), threefold, and hyaluronic acid (HA), 10-fold, were found in ML IV fibroblast extracts when compared to normal controls. Such accumulations altered the distribution of MPS:HA comprised 70% of total MPS in ML IV but only 30% in control cells. Intracellular sulfated MPS was observed accumulating almost linearly in ML IV fibroblasts. "Pulse-chase" experiments indicate that both HA and the sulfated MPS remain in the ML IV cells for long periods of time; in control cells, they are rapidly removed as low molecular weight, dialyzable fragments. These data suggest that the MPS accumulation in ML IV fibroblasts, is the consequence of a catabolic block, probably involving the lysosome.

Child, Preschool↗
PubMed1977

A-cell behavior in organ culture of isolated rat islets: secretion and tissue content of glucagon and insulin.

The present study was done to characterize the hormone secretion and content of isolated rat islets in organ culture dependent on the glucose concentration and the number of cultivated islets per culture bottle (10 or 30). For the calculation of the recovery rate the glucagon and insulin breakdown in the culture medium containing 10% calf serum was assayed. The glucagon recovery after incubation for 24 h at 37 degrees C varied from 2 to 92% and that of insulin from 75--105% depending on the serum batches. The cultivated islet responded to 16 mmol glucose by a significant enhancement of insulin release, whereas the glucagon release was not influenced. The glucagon release fell progressively with the duration of cultivation. 2 mmol dibutyryl cyclic AMP augmented insulin and glucagon release in the presence of 5 and 16 mmol glucose and significantly enhanced the glucagon content in the cultivated islets.

Animals↗
PubMed1977

Successful cultivation of isolated islets of Langerhans without attachment: relationship between Glucose- and theophylline-induced insulin release and insulin content in rats islets after cultivation.

The effect of various inhibitors of insulin secretion such as mannoheptulose (20 mM), atropine (1 mM), diphenylhydantoin (20 microng/ml), high concentration of Mg++ (5.3 mM) in the presence of 20 mM glucose (control) on insulin content and secretion from collagenase-isolated rat pancreatic islets was studied in vitro by cultivation of islets up to 5 or 9 days in glass Petri dishes without attachment. In a following short-term incubation for 60 min the glucose-induced insulin release without and with theophylline (5 mM) was investigated. Islets cultivated at 5 mM glucose and at 20 mM glucose with the inhibitors mannoheptulose or atropine lost the responsiveness to glucose and theophylline whereas such islets cultivated at 20 mM glucose alone or with diphenylhydantoin (DPH) or 5.3 mg Mg++ showed a stimulation of insulin secretion by glucose and theophylline. Compared, however, with freshly isolated islets all cultivated islets were restricted in their maximal glucose response and this defect was not evoked alone by quantitative changes in islet insulin content. Nevertheless, culture conditions which facilitate a net increase of insulin (content and release) during cultivation influenced also positively the glucose-induced insulin release without and with 5 mM theophylline in the following short-term experiments.

Animals↗
PubMed1977

[Prostate carcinoma: determination of progression following high-voltage therapy (author's transl)].

The fourth report of our long-term study concerns 70 cases of primary radiotherapy. The side effects of our radiation technique are minimal and temporary; in over 80% of the cases, potency was not affected. The morphologically demonstrable changes in the tumor tissue, in the original glandular parenchyma, and in the fibromuscular stroma leading to the vessels following high-voltage therapy are described. Three types of progressive patterns, based on the grade of histologic regression, can be determined. In more than half of the cases, with increasing distance from the source of radiation, no tumor or only minimal residual tumor was demonstrable. Sensibility and resistance of carcinoma of the prostate are not dependent upon the grade of differentiation of the tumor. A prognostic statement concerning the success of radiation therapy from initial histologic findings is not possible here. The occurrence of metastases in 18% of the cases following radiation probably resulted from the inexactness of the purely clinical determination of the stage of the tumor. The isolated, local treatment of prostate carcinoma through radiotherapy is justified only after exact determination of the stage of the tumor, i.e., certain exclusion of metastases via a diagnostic lymphadenectomy.

Adenocarcinoma↗
PubMed1977

Basal hyperinsulinemia in Wistar-rats rendered obese by a high-fat diet.

Basal plasma insulin levels were measured in Wistar-rats rendered obese by feeding a high-fat diet (50% fat, w/w). During the period of rapid weight-gaining ("dynamic phase" of the development of obesity) fat-fed rats show elevated basal insulin levels in the peripheral blood plasma. No further enhancement occurred in the so-called "static phase" of obesity. Changing the feeding schedule at 20 weeks of age, e.g. feeding control diets (3% fat, w/v) for 4 weeks to fatty rats, results in a depression of insulin levels to control values together with a reduction of the body weight. The findings are discussed in relation to the dietary induced alterations of carbohydrate and lipid metabolism of obese rats.

Animals↗
PubMed1976

Galactorrhea-amenorrhea: psychological interaction with neuroendocrine function.

Further substantiation of the interaction between psychological disease and the onset of galactorrhea-amenorrhea is presented in women who underwent intensive psychometric and neuroendocrine evaluation prior to the use of the dopamine against 2-Br-alpha-ergocryptine (CB-154). Normalization of a previously distorted psychometric test profile in each woman was accompanied by a profound decline in prolactin and an unexpected alteration in the peripheral concentrations of norepinephrine and dopamine-beta-hydroxylase. Cesation of galactorrhea and resumption of menses were observed in each instance.

Adult↗
PubMed1976

[Relations between portal and peripheral venous insulin, proinsulin and glucagon concentrations after oral glucose in man].

During cholecystectomy a catheter was inserted into the V. porta via V. umbilicalis. 7 or 8 days later an oral glucose tolerance test was performed (100 g). Blood glucose, insulin (IRI), proinsulin-like material (PLM), and pancreatic glucagon (IRG) concentrations were determined in portal as well as in peripheral venous blood. 5 out of 6 patients showed a disturbed carbohydrate tolerance in comparison to 53 healthy subjects. The IRI increased promptly after the oral glucose load. The portal IRI-concentrations are significantly enhanced in comparison to the peripheral levels, but between both a positive correlation exists (r = 0.9447; p less than 0.01). PLM was slightly increased in the first 10 minutes. The basal IRG levels were not enhanced. Significant differences between the portal and peripheral IRG concentrations could not be observed. The correlation coefficient was 0.8902. Thus, the peripheral venous concentrations of IRI, PLM and IRG can be used as a diagnostic tool of pancreatic hormone secretion in man.

Administration, Oral↗
PubMed1976

Investigations on isolated islets of Langerhans in vitro. XIII. Experiments concerning the preparation conditions with collagenase.

During the digestion of pancreatic pieces with collagenase for prepartion of isolated islets the enzymes in incubation medium (collangenolytic and/or proteolytic) can alter the secretion behavior of A- and B-cells. Insulin release after such an enzymatic attack is characterized by an enhanced basal secretion and a diminished and delayed glucose response. Overdigestion results in a decreased glucagon secretion in response to arginine, a diminished insulin content, and a decreased thiol-protein-disulfide-oxidoreductase activity of the islets. Increased albumin concentrations did not prevent the collagenase effect.

Animals↗