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Biomedical subjects

M Zhang

Publications and source records attributed to M Zhang.

At least 1,027 records · Page 57Linked to original sources
PubMed1990

[Experimental studies on treating anti-anaphylactic asthma with a qingfei bushen decoction].

On the basis of satisfying clinical effects of Qingfei Bushen Decoction, the authors carried out experimental studies with animals on anti-anaphylactic asthma with the decoction (200% condensed) to probe into the effective mechanism. The experimental results showed that the condensed decoction could effectively suppress rats' passive cutaneous anaphylaxis and guinea pigs' anaphylactic asthma allergized by egg protein. The results also showed that the condensed decoction obviously resisted the guinea pigs' bronchial spasm induced by histamine and acetylcholine, and it could significantly promote the multiplication of rats' thymus gland. Thymus gland multiplication and immunomodulating action are also discussed in the paper.

Acetylcholine↗
PubMed1990

[Clinical study of 30 cases of affective disorder with mental retardation].

Thirty cases of affective disorder with mental retardation (MR) were reported. The prevalence of affective disorder, clinic characteristics and cause of misdiagnosis in these patients were discussed. Authors found that the criteria of affective disorder set by Chinese Medical Association (1984) can be used in the patients with mild or moderate MR. Typical elated mood and flight of idea were rare. The major symptoms of these patients include hyperactivity or hypoactivity, hyperplasia or bradylogia, and increase or decrease of instinctive behavior. The atypical symptoms in manic-depressive psychoses are more common in these cases. In the most of these patients, the good result has been found.

Affective Disorders, Psychotic↗
PubMed1990

[Detection of sorbitol content in crystalline lens of normal rats and rats with diabetic cataract by 1H-NMR].

The pulse Fourier NMR was employed to measure the artificial diabetic cataract lens at various stages of its formation, and the lenses of the normal rats. Data obtained by using this method show that all the peaks that of water concentrate in the range of delta less than 4 ppm. The peak value at delta = 3.20 ppm is on a marked increase during the formation of cataract which is caused by the phosphate metabolites, such as GPC, ATP, ... etc, in cataract lens. With the development of the disease, the peak width at delta = 3.73 ppm becomes greater and greater, which shows that the activity of sorbitol dehydrogenase has decreased. This leads to a high concentration of the sorbitol in the cataract lens. Consequently, the osmosis pressure in the cataract lens is increased, and excessive water might dip into the crystalline lens to keep the balance of the osmosis pressure. And this might result in the hydration of the fiber cell of the crystalline lens, which might cause a swelling or blisters. These results are in favour of the prolongation of the relaxation time of cataractous lens reported in our other papers, and also support those gained by biochemical studies issued in the medical literature.

Animals↗
PubMed1989

The factor IX BamHI polymorphism: T-to-G transversion at the nucleotide sequence -561. The BamHI/MSPI haplotypes in blacks and Caucasians.

The polymerase chain reaction (PCR) method was used to amplify a 356-bp DNA segment containing the suspected BamHI polymorphic site of the factor IX gene. Following the enzyme digestions and gel electrophoresis, polymorphic genotypes (-,+ and +/- types) were observed. The gene frequencies for the rare (+) allele are about 36% in blacks and 2% in Caucasians. The 356-bp DNA was further purified and sequenced. The sequencing gels revealed a single nucleotide substitution (T to G) at position -561 of the gene in blacks and Caucasians. The T-to-G transversion generated a new BamHI site (GGATCC,+type) from a nonenzymatic site, (TGATCC,-type).

Base Sequence↗
PubMed1989

[Contribution of EEG to the diagnosis of epilepsy in Shanghai].

The electroencephalographs (EEGs) of 9963 patients examined for some form of neurological disorder were studied. The male:female ratio was 129:1 and the age range of 11-40 years accounted for 67.12% of those studied. Of 3914 clinically confirmed epileptic patients, 68.37% had an abnormal EEG characterized by major general or focal discharges. 50.56% of 3814 suspected epileptic patients exhibited high levels of abnormal discharge and 452 non-epileptic patients also exhibited abnormalities. Such discharges also occurred in 671 patients suspected of other neurological disorders.

Adolescent↗
PubMed1989

Three point mutations in the factor IX genes of five hemophilia B patients. Identification strategy using localization by altered epitopes in their hemophilic proteins.

In five patients with hemophilia B and detectable Factor IX antigen, altered reactivity to a specific polyclonal antibody fraction or monoclonal anti-Factor IX antibodies was noted. Amplification of selected portions of their Factor IX genes by polymerase chain reaction allowed rapid identification of a single base transition in each of the five families tested. In a patient with severe hemophilia and an altered calcium binding domain, a G to A transition in exon II changed the codon for Glu-27 to Lys (Factor IXSeattle 3). Patients from two families with mild hemophilia with decreased reactivity to a MAb that binds to a site within the sequence coded for by exon IV had a G to A transition changing the codon for Gly-60 to Ser (Factor IXDurham). Two unrelated patients with moderately severe hemophilia lacked reactivity to another murine monoclonal anti-Factor IX which binds to an epitope in the amino-terminal half of the heavy chain of Factor IXa. In these patients, exon VIII contained a G to A transition changing Arg-248 to Gln (Factor IXSeattle 4).

Adult↗
PubMed1989

[The relationships between thymus, other immune organs and various diseases in children (analysis of 621 cases)].

Thymuses, spleens, lymph nodes, tonsils and appendices from 621 autopsy cases were reviewed. The degree of atrophy of these organs might be classified into 3 grades. The main diagnoses of these cases involved more than 130 different diseases, which could be divided into 9 groups: infection, benign tumor, malignant tumor, deformity, amniotic fluid aspiration, fungal infection, autoimmune disease and hemorrhage, etc. Cases of infection with a course less than 5 days showed mild atrophy of the thymus and those cases with a longer course might show moderate or severe degree of atrophy. Cases of benign tumor showed no relationship between the degree of atrophy and the course of the disease. Nevertheless, in cases of malignant neoplasms even there was a history of short course, showed severe atrophy of the thymus. Only mild atrophy was obtained in those patients with deformity, amniotic fluid aspiration and hemorrhage, while severe atrophy was seen in those cases with fungal infection and autoimmune disease. In 81% of the cases, the degree of thymus atrophy was in accordance with those of the other immune organs.

Adolescent↗
PubMed1989

[Comparison of effect in the change of respiration by stimulating three trigeminal subnucleus].

Experiments were performed on 31 rabbits. After recovery from surgical anesthesia the change of respiration were observed in various experimental conditions, under which original nucleus of trigemini (ONT), terminal nucleus of trigemini (TNT), spinal tractus nucleus of trigemini (SNT) were respectively stimulated by an electrical current (EC) or monosodium glutamate (MSG) or lidocaine (LID). Because SNT had a longitudinal long shape, three different sections of SNT were selected as stimulating points respectively, namely rostral point (A2.33 mm), middle point (AP0 mm) and caudal point P1.9 mm). Phrenic nerve discharge and its integrated curve, PCO2 and blood pressure were recorded by a RM-6000 polygraph. The results indicated that the stimulations of EC and MSG to SNT and TNT enhanced respiratory activity obviously. Stimulation of EC and MSG to ONT caused much less enhancement of respiration. Microinjection of LID to TNT and ONT decreased respiratory activity, but microinjection of LID to SNT increased. The present experiment concluded that stimulation of SNT and TNT could cause significant change in respiration.

Animals↗
PubMed1989

[Observation on enhanced adhesion to dentin by amino acid].

An amino acid was adopted to pretreat the surface of dentin. The bond strength between GP adhesive and dentin mediated by the amino acid was enhanced after the pretreatment. Furthermore, the longer the stored time of extracted tooth was, the better the effect of adhesion was. This pretreatment will benefit the adhesive restoration for dead tooth or low activity tooth clinically.

Adhesiveness↗
PubMed1989

[A study on the promotion of adhesion properties of adhesives to metals treated by positive oxidation].

The adhesion to three alloys: Durabond, GH30 and soft Co-Cr, by PE adhesive was investigated. The surfaces of metals were treated by polishing, sand-blasting, positive-oxidation and complex processing in order to test the tensile bond strengths between metals and PE adhesive; to study the effects of the surface treatment of metals on adhesive properties; and to compare the tensile strength with that from the control experiment with EB composite resin and Panavia-EX resin under the same conditions. The results showed the mean tensile bond strengths between PE adhesive and Durabond, GH30 and soft Co-Cr alloys after the treatment of sandblasting and positive oxidation were 214.65 +/- 51.68 kg/cm2, 372.88 +/- 21.88 kg/cm2 and 349.21 +/- 48.32 kg/cm2 respectively, displaying better adhesive properties and stability of resistance to water.

Adhesives↗
PubMed1988

The Sino-MONICA-Beijing Study: report on results between 1984 and 1986.

The Beijing Heart Lung and Blood Vessel Medical Center has participated in the World Health Organisation MONICA project since 1981. Our study, named Sino-MONICA-Beijing, has been carried out in Beijing and covers a population approximately of 700,000. After a 2-year pilot study, the project started formally January 1, 1984. This paper presents the organization and methodology of the study, with emphasis on the monitoring system and on how events were found. The main results of the cardiovascular disease (CVD) event monitoring are as follows: average annual mortality from all causes, for the 3-year period of 1984-86 was 319/100,000 for men and 238/100,000 for women aged 25-64; from CVD 119/100,000 for men and 101/100,000 for women; from ischemic heart disease 29/100,000 for men and 14/100,000 for women; and from cerebrovascular disease 59/100,000 for men and 52/100,000 for women. Preliminary experience has shown that Sino-MONICA-Beijing is a well-standardized project of long-term surveillance of CVD in the communities of Beijing.

Adult↗
PubMed1988

Useful Host-Vector Systems in Bacillus stearothermophilus.

We isolated a highly transformable thermophile, Bacillus stearothermophilus SIC1, which exhibited the following features. The growth temperature ranged from 45 to 65 degrees C in L broth. The maximum cell concentration in 2L broth (2% tryptone, 1% yeast extract, 0.5% NaCl, pH 7.2) was determined as an optical density at 660 nm of 7.8, and the generation time was 11 min at 60 degrees C. Strain SIC1 was a prototroph and was transformed by the protoplast procedure not only with repB plasmids (high-copy-number plasmids such as pTB913 and pUB110) but also with repA plasmids (low-copy-number plasmids such as pTB53). Transformation efficiencies with repB and repA plasmids were about 2 x 10 to 5 x 10 and 5 x 10 transformants per mug of DNA, respectively. The transformant carrying plasmid pTB913Y/K could grow at 63 degrees C in the presence of kanamycin. The regeneration frequency of protoplasts was 60%, and only 1 day was needed for regeneration at 55 degrees C.

Journal Article↗
PubMed1988

[Snoring].

Explore the source record for details and available documents.

Humans↗
PubMed1987

Several nodulins of soybean share structural domains but differ in their subcellular locations.

Four soybean cDNA nodule-specific clones encoding nodulin-23, -26b, -27 and -44 were observed to cross-hybridize under low stringency conditions. Nucleotide sequence analysis revealed that the cDNAs contain three distinct domains: two domains with 70 to 95% homology separated by a third domain unique to each cDNA. Despite a number of nucleotide insertions and deletions, the protein sequences are conserved in the two domains which correlate with the homologous nucleotide domains. The amino terminal domain of each nodulin contains putative signal sequences for membrane translocation, although only two (nodulin-23 and -44) meet all the criteria for a functional signal. Immuno-precipitation of hybrid-release translation products of the four cDNAs revealed that nodulin-23 is associated with the peribacteroid membrane while nodulin-27 is in the cytoplasmic fraction of the nodule. These four nodulins are members of a diverse family with conserved structural features and the genes encoding them appear to have recently evolved from a common ancestor.

Amino Acid Sequence↗