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Biomedical subjects

M Zhang

Publications and source records attributed to M Zhang.

At least 991 records · Page 55Linked to original sources
PubMed1992

[Pharmacological study on spleen-stomach warming and analgesic actions of Aconitum carmichaeli Debx].

This study presents an observation of the anti-inflammatory effect of Aconitum carmichaeli decoction on water-immersion stress induced gastric ulcer in mice and 0.6 mol/L HCl induced gastric ulcer in rats. The observation showed that the decoction was resistant to the castor oil induced and Cassia angustifolia leaf induced experimental diarrhea in mice, and also had marked analgesic action. It is thus suggested that Aconitum carmichaeli is useful clinically as a spleen-stomach warming and analgesic agent in traditional Chinese medicine.

Analgesics↗
PubMed1992

[The use of 6-flavor Rehmannia decoction with additives in the prevention of ototoxic deafness induced by gentamicin in guinea pigs].

The purpose of this study is to discuss the effects of the Chinese medicine six-flavor Rehmannia Decoction with additives on preventing deafness induced by gentamicin (GM) in guinea pig by means of testing the thresholds of Preyer's reflex (PR) cochlear microphonic potential (CM), action potential of the auditory nerve (N1) and scanning electron microscope of inner ear (SEM). The results showed that the medicine is effective in reducing the ototoxic effect of GM on inner ear.

Action Potentials↗
PubMed1992

Tube-perilymph fistula. An experimental study.

Before exploratory tympanotomy, it is difficult to diagnose an idiopathic perilymph fistula (PLF) because of its variable presentations. An animal model tube-PLF on guinea pigs was established to evaluate the change of the sensitivity of the cochlea function and the value of the postural audiometry in PLF. A fine polyethylene tube (O.D. 0.6mm) was inserted into the scala tympani of the basal turn through the incised round window membrane in 10 experimental guinea pigs, and another 5 as controls. The hearing threshold (HT), action potential (AP) latency for stimulus of 105 dB SPL, difference of HT and AP latency from the test ear down to up were measured by electrocochleography (ECoG) immediately after operation and one week later. The HT, AP latency and postural audiometry showed no significant changes immediately but a significant difference one week later in HT (P less than 0.01) and postural audiometry, the AP latency also presented a significant delay (P less than 0.01). The results showed that the tube-PLF model may be a valuable method for studying chronic PLF.

Action Potentials↗
PubMed1991

Improved serodiagnosis of hepatitis C virus infection with synthetic peptide antigen from capsid protein.

Cloning and expression of hepatitis C virus have allowed the development of immunoassays to detect hepatitis C virus infection. However, currently available recombinant fusion protein C100-3 assays, based on a nonstructural protein of the virus, are limited in sensitivity, particularly for detecting acute infection. In this report seroconversion panels showed that an assay based on synthetic peptides, derived from immunodominant regions of both capsid and nonstructural proteins, accelerated hepatitis C virus antibody detection by 4-10 weeks. In screening, this enzyme immunoassay increased detection from 47% to 64% in plasmapheresis donors with elevated alanine aminotransferase levels (greater than 100 international units per liter), from 15% to 24% in anti-hepatitis B core antigen-positive blood donors, and from 28% to 42% in renal dialysis patients when compared with nonstructural peptide-based assays. The screening assay was repeatedly reactive for 27 of 2902 volunteer blood donor samples (0.93%); four sera reacted only with the capsid antigen. The peptide test distinguished true from false positive results in agreement with recombinant immunoblot assay in 96% of blood donor samples repeatably reactive on a recombinant hepatitis C virus enzyme immunoassay.

Animals↗
PubMed1991

A yeast protein with homology to the beta-subunit of G proteins is involved in control of heme-regulated and catabolite-repressed genes.

The product of the Saccharomyces cerevisiae AER2 gene is responsible for maintaining repression of at least two distinct regulatory pathways: heme activation/repression and catabolite repression. Mutations in the gene caused an eightfold increase in the expression of the heme-activated CYC1 gene in the absence of heme, a substantial increase in the expression of the heme-repressed ANB1 gene in the presence of heme, and a 13-fold increase in the expression of the catabolite-repressed GAL1 gene in the presence of glucose. Lesser or no increases in the expression of these genes were observed under derepressed or activation conditions. The aer2 mutations also caused a large increase in CYC7 gene expression under all conditions; this gene is subject to heme activation/repression, as well as catabolite repression. The AER2 gene was cloned and the sequence determined. The large open reading frame contiguous with the transcript from the complementing region encoded a 713-amino acid polypeptide chain with extensive homology to the beta-subunit of G proteins. The sequence revealed that AER2 is the TUP1 gene. A deletion mutation was constructed and the null phenotype was the same as the original mutants. The aer2 null mutant was shown to have increased aerobic and anaerobic levels of RNA encoding the ROX1 repressor, normally expressed only aerobically and responsible for the aerobic repression of ANB1 expression. The increase in both ROX1 and ANB1 RNAs aerobically in this mutant suggests that the repressor is nonfunctional in the mutant.

Amino Acid Sequence↗
PubMed1991

CG dinucleotide transitions in the factor IX gene account for about half of the point mutations in hemophilia B patients: a Seattle series.

Hemophilia B is due to multiple molecular defects in the factor IX gene. Over 80% of mutations are single base substitutions. By amplification and direct sequencing, 51 single base substitutions were found in the transcribed sequence of the factor IX genes of patients from 50 distinct families with hemophilia B. These include 30 mutations in 29 families not previously reported by us; of these, 12 are novel, i.e., not previously published in other series. Of the 51 substitutions in our overall series 23 (45%) occurred as C-to-T or G-to-A transitions at 11 sites within CG dinucleotides. It is estimated that CG transitions occur from one to two orders of magnitude more frequently than mutations in nucleotides that are not within a CG pair. More than one family had identical defects for 6 of the CG mutations. At 4 of these sites, most patients had different haplotypes compatible with distinct mutations. Non-CG-type mutations occurred throughout the coding regions with only one mutation in more than one family. The latter included 7 families with a 397 Ile-to-Thr defect that all share a rare haplotype, suggesting a common ancestor.

Amino Acid Sequence↗
PubMed1991

[Pharmakinetics of carboplatin in cancer patients].

Pharmakinetics of carboplatin was studied in 6 cancer patients who received carboplatin (300 mg/m2) by 30 minute intravenous infusion. Platinum plasma concentration was measured by reverse high performance liquid chromatography. The results showed that total platinum plasma clearance was biphasic in all patients with a short initial phase half-life of 1.27 hrs and a long second phase half-life of 6.92 hrs. The distribution volume of the drug was 57.78 L/m2 and area under the total platinum concentration curves was 84.71 mg/L.hr.

Adult↗
PubMed1991

Hepatic artery ligation and infusion chemotherapy for unresectable primary liver cancer.

During the period of 1958-1989, 356 patients with pathologically proven primary liver cancer (PLC) were determined by laparotomy to be unresectable. Of the 356 patients, 51 (14.3%) were of subclinical stage, 287 (80.6%) of moderate stage and 18 (5.1%) of late stage. The association of liver cirrhosis was present in 310 patients (87.1%). Treatment modalities in 356 patients were divided into 4 groups: hepatic artery ligation (HAL) (51), hepatic artery infusion (HAI) of chemotherapeutic agents (114), HAL + HAI (117), and HAL + HAI + radiotherapy (74). The 5-year survival rate was zero in the 4 groups in the period of 1958-1977. During 1978-1989, however, the 5-year survival rate was zero in HAL, 7.9% in HAI, 24.4% in HAL + HAI (with second look resection in 10 patients), and 36.5% in HAL + HAI + radiotherapy (with second look resection in 19). The marked improvement in survival in later period was attributable to the accurate site of hepatic artery catheter, longer infusion chemotherapy, and combination treatment, particularly second look resection in some of the patients. These results indicate that HAL + HAI + combination treatment might provide a possible prolongation of survival or even resection in some patients with original unresectable PLC.

Adolescent↗
PubMed1991

[Pharmacological studies on warming the middle-jiao to alleviate pain by Pericarpium Zanthoxyli].

The pharmacological effects of the ethereal extract and aqueous extract of Pericarpium Zanthoxyli on warming the middle-jiao to alleviate pain have been studied. It is suggested that the effects of Pericarpium Zanthoxyli on antagonizing ulcer, and inflammatory and functional diarrheas, protecting liver, and correcting gastrointestinal disorder are the connotation of its warming the middle-jiao to alleviate pain, its analgesic effect helps to relieve various abdominal pains.

Alanine Transaminase↗
PubMed1991

[Textual research on luohuangzi].

By studying ancient and modern literatures and observing the material object, we have come to the conclusion that the fruit called "luohuangzi" in ancient times should be the seed of Sterculia nobilis rather than Tamarindus indica. We therefore suggest that "luohuangzi" be no longer used as another name for T. indica, and, in order to eliminate the confusion about the name and material object of "luohuangzi". Tamarindus should be called the "genus Shuandou" in Chinese instead of the "genus Luowangzi".

Drug Contamination↗
PubMed1991

[Isolation and identification of cynanester A from Cynanchum chinense R. Br].

Cynanester A (I) a new triterpenoid ester was isolated, together with lupeol acetate, lignoceric acid and beta-sitosterol, from Cynanchum chinense R. Br. (Asclepiadaceae). The mass spectrum suggested the presence of caproyl group and lupane skeleton with isopropenyl group in the molecule. Alkaline hydrolysis of I with 5% methanolic potassium hydroxide yielded lupeol Ia and n-caproic acid. The acid was esterified with MeOH-H2SO4 to afford methyl caproate, by GLC comparison with authentic sample. The acid was also identified by IR comparison. As above, the structure of cynanester A was established as lupeol caproate.

Caproates↗
PubMed1991

[Effect of rhizoma Zingiberis on anoxic or cooled mice].

The anti-anoxic experiments have indicated that the petroleum ether extract of Rhizoma Zingiberis can prolong the survival time in mice poisoned by KCN or under the condition of normobaric hypoxia, as well as decapitation-induced gasping duration in mice, but can not prolong the survival time in mice poisoned by NaNO4. The anti-anoxic action might be related to the reduction rate of O2 consumption of the body. The aqueous extract of Rhizoma Zingiberis does not possess the above-mentioned anti-anoxic action. Both extracts do not affect the survival time in cooled mice.

Animals↗
PubMed1991

Enhancement of the cytotoxicity of mistletoe lectin-1 (ML-1) by high pH or perturbation in Golgi functions.

We have studied the cytotoxicity of Mistletoe lectin-1 (ML-1), a cytotoxic protein produced by Viscum album, in CHO and V79 cells and in mutant cell lines altered in Golgi functions or in endosomal acidification. In wild-type CHO cells, cytotoxicity of ML-1 was greatly enhanced by ammonium chloride or nigericin. A CHO mutant defective in endosomal acidification (DMPR-2), which is resistant to diphtheria toxin, modeccin and Pseudomonas aeruginosa exotoxin A and hypersensitive to ricin, showed increased sensitivity to ML-1. MonR-31 and MF-1 are monensin- and compactin-resistant mutants derived from CHO and V79 cell lines, respectively, and are presumably altered in Golgi functions. The cytotoxicity of ML-1 was found to be increased in both MonR-31 and MF-1 cells as compared with their parental cells. These results indicate that the effects of chemicals or mutations altering endosomal acidification and Golgi functions on the cytotoxicity of ML-1 are similar to those on ricin cytotoxicity. Our results suggest that the cytotoxicity of ML-1 is enhanced by an increase in endosomal pH, as well as by chemicals or mutations altering the structure/functions of the Golgi regions. Like ricin, the intoxication process of ML-1 may involve the Golgi regions.

Ammonium Chloride↗
PubMed1991

[Studies on warming the middle-jiao and analgesic effect of flos Caryophylli].

The effects of water extract and ether extract of Flos Caryophylli have been investigated by four experimental gastric ulcer models, choleresis in rats, two diarrhea models in mice, two pain stimulations in mice, and acute liver injury induced by CCl4 in rats. The results suggest that the action of Flos Caryophylli on dispelling the cold by warming the middle-jiao and curing abdominalgia is related to its anti-ulcer, choleresis increasing, anti-diarrhea and analgesic functions.

Analgesics↗