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Biomedical subjects

M Zeller

Publications and source records attributed to M Zeller.

67 records · Page 4Linked to original sources

Continuous intra-arterial oximetry, pulse oximetry, and co-oximetry during cardiac surgery.

This study evaluated arterial catheter oximetry versus pulse oximetry in eight patients (ASA III-IV) who underwent cardiac surgery. Co-oximeter saturation values served as the standard. Arterial oxygen saturation was determined simultaneously with these three methods at 162 prospectively defined points of measurement before, during, and after cardiopulmonary bypass (CPB). At the same times before and after CPB, arterial, pulmonary arterial, and central venous pressures, and cardiac output determinations were recorded. Saturation readings were obtained in more than 99% of measurements with catheter oximetry and in only 59% to 84% of measurements with pulse oximetry. Failure of pulse oximetry correlated with low mean arterial pressures and low cardiac outputs, but not with high systemic vascular resistance. The mean saturation values determined by catheter oximetry as well as by pulse oximetry differed from the mean values obtained by co-oximetry by less than 1% (= bias). The standard deviations of the individual differences between readings of catheter or pulse oximetry and readings of co-oximetry (= precision) were +/- 0.5% to +/- 1.0% for catheter oximetry and +/- 1.0% to +/- 1.2% for pulse oximetry. In summary, catheter oximetry was superior to pulse oximetry with regard to obtaining readings and to reliability of the obtained readings. Invasiveness and high costs influence the decision as to whether to use catheter oximetry, but if reliable and precise measurements of saturation are important at any time during surgery, pulse oximetry is an insufficient method and co-oximetry is a time-consuming method of analysis, whereas catheter oximetry is quick, reliable, and precise.

Adult↗

[Intra-arterial catheter oximetry and pulse oximetry in comparison with CO-oximetry in heart surgery].

This study evaluates the measurement of oxygen saturation by arterial catheter oximetry and pulse oximetry. The values are compared to values obtained by CO-oximetry. METHODS. In eight patients undergoing cardiac surgery, we determined the oxygen saturation of arterial and mixed-venous blood by CO-oximetry (IL 282, Instrumentation Lab) at prospectively defined points of measurement before, during, and after extracorporeal circulation. At the same points of measurement, saturation readings obtained by arterial catheter oximetry (U425C, Abbott) and pulse oximetry (Siemens/Nellcor) were recorded. RESULTS. The mean saturation values determined by both catheter oximetry and pulse oximetry differed from the mean values obtained by CO-oximetry by less than 1% (= bias). The standard deviations of the readings in relation to readings of CO-oximetry (= precision) were +/- 0.5% to +/- 1.0% for catheter oximetry and +/- 1.0% to +/- 1.3% for pulse oximetry. Furthermore, it was possible to obtain saturation readings in 99%-100% of measurements by catheter oximetry; in contrast, this was possible by pulse oximetry in only 59%-84% of measurements. Low mixed-venous saturation values were not indicated by any of the arterial methods of measurement. CONCLUSIONS. Catheter oximetry was superior to pulse oximetry with regard to both precision of saturation values and reliability to obtain values. Invasiveness and high costs are disadvantages of catheter oximetry, but if reliable and exact measurements are important at any time during surgery or intensive therapy, intra-arterial catheter oximetry is preferable to pulse oximetry.

Cardiac Surgical Procedures↗

Immunohistochemical localization of galactosyltransferase in the normal human ovary and fallopian tube.

Galactosyltransferase (UDP-galactose: 2-acetamido-2-deoxy beta-D-glucopyranose beta-(1-4) transferase) in human tissue specimens from ovaries and the corresponding fallopian tubes was localized immunohistochemically for light microscopy. An affinity-purified rabbit anti-human milk galactosyltransferase antibody was used. Intracellular galactosyltransferase was found to be localized to the juxtanuclear (Golgi) region of the secretory cells of the fallopian-tube epithelium and to the ovarian stromal cells involved in steroid-hormone production. Cell-surface galactosyltransferase was localized to ciliated cells of the fallopian-tube epithelium. During the follicular phase of the menstrual cycle, galactosyltransferase was found only in the Golgi regions of theca interna cells of the ovarian graafian follicle, and in the fallopian tube was found predominantly on the cilia of epithelial cells. During the luteal phase, galactosyltransferase was abundant in the Golgi regions of granulosa lutein cells of the corpus luteum, and was predominant in the secretory cells of the tubal epithelium. Galactosyltransferase was not detected on the mesothelial ovarian surface. The results demonstrate that the cellular distribution and location of galactosyltransferase correlates with phenotypic differentiation and varies during the human female hormonal cycle.

Adolescent↗

[Endangering of health by ingestion of soft-polyvinylchloride (author's transl)].

Four trials to evaluate the pathogenicity of Soft-Polyvinylchloride were conductet by feeding pigs soft plastic objects. The plastic objects contained ca. 60-75% softening agents. Only in one trial did the plastic object remain in the pigs stomach. The object remained in the stomach 102 days and caused lesions of the mucous membranes. In the stomach the soft plastic became very hard and the projections of the object became pointed, resembling needles. The amount of softening agent in the object had been reduced from 69,8% to 42%. The danger, especially to children, after eatingf such plastics, either consciously or involontary, is therefore demonstrated. Aspects of state food laws concerning this problem are also discussed in the article.

Administration, Oral↗

Bruno Klopfer.

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California↗

Rupture of a pancreatic cyst into the portal vein. Report of a case of subcutaneous nodular and generalized fat necrosis.

Nodular subcutaneous and generalized fat necrosis, due to rupture of a pancreatic cyst into the portal vein, occurred in a 57-year-old man. No report of a similar mechanism causing such a result was found in the literature. Multiple papular lesions, with microscopic changes in the skin, although not in themselves specific, should lead to a suspicion of systemic dissemination of pancreatic secretions into the blood stream.

Acute Disease↗

Validation and quality assurance of planar chromatographic procedures in pharmaceutical analysis.

Within the process of the International Conference on Harmonization (ICH), 2 guidelines were released containing a standardized terminology, a verified model of requirements for the validation of analytical procedures, and some guidance in the practical aspects of conducting validation studies in pharmaceutical analysis. For planar chromatographic procedures, which may be used at different levels either in qualitative identity testing, assays, semiquantitative limit tests, or quantitative determination of impurities, this paper tries to transfer these formal requirements into practical approaches for validation. Basic acceptance criteria for evaluation of validation experiments based on practical experience are proposed. In addition, selected parameters for robustness testing of given procedures and quality assurance of quantitative planar chromatographic testing by control charts is described.

Chromatography↗