Search PubMed⌕ Search

Biomedical subjects

M Zaremba

Publications and source records attributed to M Zaremba.

At least 73 records · Page 4Linked to original sources
PubMed1979

Sensitivity to chemotherapeutics of Yersinia enterocolitica strains.

290 Y. enterocolitica strains were tested as regards their sensitivity to 20 chemotherapeutics. All the strains were isolated from fecal specimens taken from human beings in Czechoslovakia. It should be noted that the strains presented homologous group as they belonged to 3 serological group according to Winblad or IA according to Knapp and Thal, they also belonged to biotype 4 according to Nilehn. It has been found that apart from their homogenicity, they showed quite different patterns of sensitivity to chemotherapeutics. Among them quite a great number of the strains were of high level of resistance to antibiotics. It was not possible to transfer their resistance to the sensitive E. coli K12 strain. All the strains proved to produce betalactamase.

Anti-Bacterial Agents↗
PubMed1978

Pathogenicity of various Yersinia enterocolitica serotypes towards mice of Swiss and Balb/c strains.

Series of experiments aimed at determining LD50 of various Y. enterocolitica serotypes toward mice of Swiss and Balb/c strains infected by different route have been made. It appeared impossible to establish the LD50 in mice of Swiss strains after a single and repeated intravenous infection. Attention has been paid to a clear relationship between the mortality of Balb/c mice and culture conditions of Y. enterocolitica strains used for infection. In regard to particular serotypes LC50 could be determined on different days of observation and with various routes of infection of Balb/c mice. However, it concerned only those mice groups which were given suspensions of bacteria or broth culture incubated for 72 hrs at 22 degrees C. Irradiated Balb/c mice were more susceptible to infection with all serotypes of Y. enterocolitica. In the course of oral infection of Balb/c mice with serotypes IA and V of Y. enterocolitica it was possible to check the development of pathological lesions in gut and other organs, this did not refer, however, to Y. enterocolitica serotypes IB, II, III, IV, and VI.

Animals↗
PubMed1978

Humoral and cellular immune reaction in Balb/c mice infected with Yersinia enterocolitica.

Experiments were carried out on Balb/c mice infected with Y. enterocolitica serotypes IA, IB, II, III, IV, V and VI (according to Knopp and Thal) to check humoral and cellular immune response both primary and secondary. It was shown that the occurrence of anti-OH agglutinins and their dynamics depends on the serological type of Y. enterocolitica used for infection, and that it is related to the primary and secondary challenge. The presence of anti-OH agglutinins and hemagglutinins was observed only in cases of secondary infection of the mice. Delayed hypersensitivity evaluated as a cellular reaction assayed with a capillary test of spleen macrophages migration was found only in the cases of infection with serotypes IB, II, IV and VI. The inhibition of macrophage migration was not observed in the cases when infection of Balb/c mice had been evoked with Y. enterocolitica serotypes IA, III and V.

Animals↗
PubMed1978

The usefulness of various Yersinia enterocolitica antigens for passive hemagglutination test.

Using various antigen preparations of seven Yersinia enterocolitica serotypes (according to Knapp and Thal) and homologous rabbit immune sera the author succeeded in standardization of passive hemagglutination test. The usefulness of preparations termed "supernatant" derived from heated cells at 100 degrees C and lyophilized "crude" preparations of all serotypes were assessed using blood donors' sera and sera of subjects suspected of infection with Y. enterocolitica. It has been shown that though the diagnostic value of supernatant is higher than that of crude fraction, with the former one it is not possible to differentiate hemagglutinins against particular serotypes. More detailed assessment has been limited to serotype IA, only. An equal usefulness for passive hemagglutination of preparations termed "CA free crude" and "ethanol insoluble fraction" derived from TCA extracted cell sediment has been found.

Antigens, Bacterial↗
PubMed1978

Standardization of the agglutination test in the diagnosis of Yersinia enterocolitica infection.

For preparation of antigens standard strains of Yersinia enterocolitica representing serological groups I to VI (according to KNAPP and THAL) were used. The following antigens preparations were used: antigens autoclaved at 121 degrees C, antigens treated with formaline and living cells. The antigens were titrated against homologous immune sera, and their optical density was estimated spectrophotometrically. In the rabbit immune sera, and additionally, in the sera of blood donors and subjects suspected of Y. enterocolitica infection the levels of agglutinins were estimated against the antigens of all serotypes. The results from both groups of sera were analysed numerically with the use of the "Odra 1204" computer. Basing on the antigen titers frequency distribution critical and diagnostically doubtless values were established. The usefulness of living cells and antigens treated with formaline of the serotype IA and serotype V in the serological diagnosis of the Y. enterocolitica infections was demonstrated.

Agglutination Tests↗