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Biomedical subjects

M Young

Publications and source records attributed to M Young.

At least 433 records · Page 24Linked to original sources
PubMed1975

Changes in plasma non esterified fatty acids and serum glycerol in pregnancy.

Changes in plasma nonesterified fatty acids (NEFA) and serum glycerol in pregnancy were examined in a semi-serial study of 85 women. A preliminary study showed that it was almost impossible to achieve standardized conditions for sampling, and the compromise of taking a single venous sample without stasis, after 30 minutes rest and about 12 hours fasting, was adopted. Although there were large individual variations the patterns of change were, in general, the same for NEFA and glycereo although the two were not closely correlated. There was no +convincing change before 30 weeks of pregnancy but both increased in the last ten weeks, fell sharply in the first week post partum, and then rose again to late pregnancy levels by 6 weeks post partum before falling to non-pregnant levels by between 3 and 6 months post partum. Those patterns of change are in broad accordin with changes of fat storage and lipolysis associated with the reporoductive cycle. Changes in the patterms of NEFA were triviax the only significant alteration was a small rise in the proportion of C16:0 (palmitic) in late pregnancy.

Chromatography, Gas↗
PubMed1975

Genetic mapping of sporulation operons in Bacillus subtilis using a thermosensitive sporulation mutant.

A thermosensitive sporulation mutant was used to determine the order of sporulation operonsin the urs region of the Bacillus subtilis chromosome. Data from three-factor transformation crosses and three- and four-factor transduction crosses established the order metC-SPO-96(SpoII)-spo-85(SpoV)-spo-279(SpoII)-furA-ura-cysC-spo-NG1.67(SpoIII). Previously, furA was thought to lie to the right of ura and cysC to the left (Dubnau, 1970; Young and Wilson, 1972).

Alkaline Phosphatase↗
PubMed1975

The influence of extraneous factors on Coulter S measurement of the mean corpuscular volume.

Alteration in the temperature of the Isoton diluent in a Coulter model S counter over a range of possible laboratory working temperatures produced a change in the mean corpuscular volume using EDTA and dipotassium acid citrate dextrose blood and a commercial control, 4C. Contamination of the Isoton diluent with hypochlorite solution, used to clean the Coulter model S, may increase the mean corpuscular volume of EDTA blood without altering other measured parameters. Attention to both these points is required in assessing quality and calibrating the machine.

Blood Cell Count↗
PubMed1975

Storage of blood and the mean corpuscular volume.

The mean corpuscular volume (MCV) of blood after storage for up to 24 days under various temperature conditions in ethylene diamine tetracetic acid, acid citrate dextrose, citrate phosphate dextrose, and Alsever's solution was measured using the Coulter S. Storage of blood overnight at 23 degrees centigrade increased the MCV, while there was no significant change after similar storage at 4 degrees C. Storage of blood for 23 days at 4 degrees C for 24 days. Similar results were found with all anticoagulants tested. The precision of the MCV varied on storage with different anticoagulants.

Anticoagulants↗
PubMed1975

The influence of diets deficient in energy, or in protein, on conceptus weight, and the placental transfer of a non-metabolisable amino acid in the guinea pig.

The effect on the guinea pig conceptus of feeding the mother diets deficient in energy or in protein has been investigated. Fetal uptake of a labelled non-metabolisable amino acid, amino-isobutyric acid, injected into the maternal blood stream, was studied at the end of gestation. Both diets retarded fetal growth, influencing litter number and fetal and placental weight. The relationship between fetal and placental weight was similar in the control and restricted-calorie animals, and the transfer studies showed that placental function and fetal uptake per unit weight was similar in the two. Feeding a low-protein diet caused the ratio between fetal and placental weight to increase; enhanced placental transfer and fetal uptake also suggested the possibility of adaptation when the calorie supply is adequate.

Aminoisobutyric Acids↗
PubMed1975

A comparison of gas-liquid chromatography and a titration method for the determination of plasma non-esterified fatty add levels during pregnancy.

The level of plasma non-esterified fatty acids (NEFA) was measured by gas-liquid chromatography (GLC) and a titration method in 194 samples collected during pregnancy and from four days to 24 weeks post partum. Both techniques indicated a similar pattern of changes in plasma NEFA associated with pregnancy. The titration estimates of NEFA level were usually greater than those measured by GLC, and there was some suggestion that the disparity between the methods was increased at the end of pregnancy and was reduced at six weeks after delivery.

Fatty Acids, Nonesterified↗
PubMed1975

Effect of subvocalization on memory for speech sounds.

Subjects in two groups were presented with sequences of 6 consonant-vowel syllables for ordered recall. Subjects in both groups were trained to suppress subvocalization. Only subjects in the feedback group were asked to suppress subvocalization during the experiment, while subjects in the no-feedback group were allowed to subvocalize during the memory task. Analysis of the electromyographic records showed a large decrease in subvocalization in the feedback condition, and results of the memory task revealed an increase in errors for this group. However, a comparison of errors within each group revealed an identical pattern of overall errors and intrusion errors on subvocalization and nonsubvocalization trials, and these results were identical in both groups. The results suggest that requiring subjects to simultaneously suppress subvocalization and remember syllables depresses performance slightly, but encoding of speech sounds in short-term memory occurs independently of subvocal activity during the memory task.

Auditory Perception↗
PubMed1974

Synthesis of nerve growth factor by L and 3T3 cells in culture.

Mouse submaxillary gland nerve growth factor (NGF) has been covalently joined to bacteriophage and the resulting phage conjugates remain biologically active in stimulating neurite extension from sensory ganglia. A sensitive bacteriophage immunoassay has been developed to measure concentrations of NGF as low as 1 ng/ml. With this method, we find that mouse L and 3T3 cells in culture produce a biologically active nerve growth factor that is immunologically similar if not identical to mouse submaxillary gland NGF. Since L cells are known to be a source of "conditioned medium" for tissue culture, it could be that one or more of the conditioning factor activities secreted by these cells are due to NGF itself.

Animals↗
PubMed1973

Nerve growth factor: relationship to the cyclic AMP system of sensory ganglia.

Nerve growth factor and N(6),O(2)' dibutyryl adenosine 3',5'-monophosphate both stimulate neurite elongation by explanted ganglia. However, the addition of nerve growth factor does not lead to increased amounts of adenosine 3',5'-monophosphate in intact ganglia, nor does it stimulate adenylate cyclase activity in broken ganglia cells.

Adenosine Triphosphate↗