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Biomedical subjects

M Yoshimura

Publications and source records attributed to M Yoshimura.

At least 865 records · Page 48Linked to original sources
PubMed1976

Immunological studies on LATS-immunogloblin by the reaction with staphylococcal protein A.

The reaction of LATS activity with Staphylococcal Protein A, a specific binding protein with the Fc part of human IgG(1), IgG(2) and Ig(4), was examined. When IgG(1), IgG(2) and Ig(4) subclasses were removed from LATS positive sera or LATS-IgG fractions by affinity chromatography on Protein A-Sepharose, LATS activity decreased. Almost all LATS activity was found in the fraction that reacted with Protein A. It is suggested that LATS has an expression of a very distinct immunoglobulin G structure, and that LATS activity is distributed mainly in the fraction containing IgG(1), Ig(2) and Ig(4) in LATS positive serum;

Bacterial Proteins↗
PubMed1976

Determination of triiodothyronine in red blood cells by radioimmunoassay.

This study was undertaken to determine T3 content in red cells by radioimmunoassay. T3 in red blood cells was solubilized fairly from the stroma by hemolysis and red-cell T3 content could be determined directly by radioimmunoassay of the lysate. After hemolysing red cells with an equal volume of distilled water, 0.4 ml of the hemolyzate was used for the assay. The red-cell T3 content was expressed as ng/ml of red-cell volume. The normal T3 range in red cells was 0.20-0.45 ng/ml, and the Mean+/-SD was 0.32+/-0.10 ng/ml. The limit of detectability was 0.2 ng/ml. In hyperthyroid patients, the red-cell T3 content was more than 0.50 ng/ml with a Mean +/-SD of 1.35+/-0.65 ng/ml. In hypothyroid patients, red cells contained less than 0.25 ng/ml of T3, and there was an overlap from 0.20 to 0.25 ng/ml in the content of red-cell T3 in hypothyroid and euthyroid subjects. The patients with T3 toxicosis showed a high or normal level of red-cell T3. A positive correlation was noted between the red-cell T3 content and the serum T3 level (r=0.66). The correlation between the red-cell T3 content and the free T4 index (expressed as T7) was also positive (r=0.67). From these experiments, it is suggested that the red-cell T3 is low in comparison with the serum T3 levels, and depends on two factors; serum T4 and serum T3 levels.

Binding, Competitive↗
PubMed1975

Enhanced growth hormone responses to TRH injection in bipolar depressed patients.

Twenty-five depressed patients were examined as to their growth hormone responses to TRH. An enhanced pituitary growth hormone response to intravenous injection of 500 mug TRH was observed in eight depressed patients, while TRH administration did not raise growth hormone levels in nine of 10 normal subjects examined. Occurrence of enhanced response of growth hormone was not related to the thyrotropin values after TRH administration. Bipolar patients exhibited enhanced growth-hormone response more frequently than unipolar patients. Five patients with involutional depression and neurotic depression who showed the most insufficient thyrotropin release to TRH administration together with lowered thyroid function revealed to be non-responders of growth hormone.

Adolescent↗
PubMed1975

Thyroid function levels and thyrotropin responses to TRH administration in manic patients receiving lithium carbonate.

To determine the pituitary-thyroid dysfunction in the manic patients receiving lithium carbonate, determination of thyroxine concentration, T3 Resin Uptake, thyrotropin concentration in plasma, and thyrotropin responses to administration of TRH, 500 mug i.v., were made. Eight manic patients were examined before and after lithium treatment, 600-1,200 mg daily, for four weeks. Slight reduction in the plasma levels of thyroxine, and slight increase in the plasma thyrotropin levels were seen following lithium treatment. Significantly enhanced thyrotropin responses to TRH administration were found as compared to the results before lithium treatment. It is assumed that lithium salts decrease both output of thyroid hormones and the sensitivity of the thyroid gland to thyrotropin, and that this action of lithium inhibiting the thyroid gland might be related to its anti-manic effect.

Adult↗
PubMed1975

Estimation of the maximal T4-binding capacity of TBG using the Triosorb test in serum treated with dextran-coated charcoal.

A new method for the estimation of the maximal T4-binding capacity of TBG (max. TBG) in serum (dextran-coated charcoal-triosorb method) was devised. Thyroxine (T4) concentration in test sera were determined by the Tetrasorb method and then T3 resin sponge uptake (T3 RSU) was determined after extraction of 70% of the endogenous T4 with dextran-coated charcoal. The binding capacity of unsaturated TBG in treated sera was estimated from the reciprocal of T3 RSU. Max. TBG was calculated from the arithmetic sum of the unsaturated binding capacity of TBG and the serum T4 concentration multiplied by 0.3. The normal range for max. TBG was 20.1 +/- 2.6 (mean +/- SD) mug T4/100 ml (14 approximately 24) and it was 19.2 +/- 3.5 mug T4/100 ml in hyperthyroidism which was lower compared with hypothyroidism (21.1 +/- 2.6 mug T4/100 ml). Max. TBG in 8 hyperthyroid patients did not change significantly after treatment in half of the case, although in the other half it was slightly increased. Increased values of max. TBG were observed in pregnancy (32.9 +/- 4.4) and in a hyperthyroid state associated with pregnancy (31.4 +/- 4.7). Decreases were observed in TBG deficiency (less than 5.6 mug T4/100 ml). These procedures can be applied for the determination of both the unsaturated binding capacity of TBG and that of max. TBG.

Female↗
PubMed1975

Serum T3 level in the patients with hyperthyroidism after therapy.

Serum T3 level in various thyroid diseases was determined in unextracted serum with the Dainabot kit for T3 RIA. The serum T3 level in 33 normal subjects was 0.8-1.6 ng/ml. It was 5.7 +/- 3.5 ng/ml (mean +/- S.D.) in 36 hyperthyroid patients, and undetectable to 0.8 ng/ml in 21 hypothyroid patients. Generally the serum T4 and serum T3 decreased in parallel after radioiodine therapy for hyperthyroidism. However, in some cases the serum T3 level remained high in spite of normalized serum T4 after radioiodine therapy. This state indicated "T3-toxicosis", and hyperthyroidism was apt to recur. When thyroid function was observed for 2 years following radioiodine treatment, the ratio of serum T3 (T3 level before treatment/T3 level after treatment) decreased more significantly as compared with the ratio of serum T4 in euthyroid cases. Serum T3 provides a more sensitive index of thyroid function than serum T4 in euthyroid states after radioiodine or anti-thyroid drug therapy. The present data indicate that the serum T3 level and the T4/T3 ratio are valuable aids in the estimation of prognosis of hyperthyroid patients after various treatments.

Adult↗
PubMed1975

Radioimmunoassay for estimation of thyroglobulin in human serum.

A specific double antibody radioimmunoassay has been develop for the measurement of thyroglobulin in human serum. Human thyroglobulin was purified by combined DEAE-cellulose and affinity chromatography using Sepharose 4B-bound Concanavalin A. Sensitivity of test serum was 10 ng/ml. Thyroglobulin was not detectable in half of normal subjects, and half showed values between 10 and 180 ng/ml. In the patients with simple goiter and secondary hypothyroidism, serum thyroglobulin was usually in the normal range. In Hashimoto's thyroiditis, many sera having precipitating antibodies or high hemagglutination antibodies for thyroglobulin showed a high thyroglobulin concentration in serum probably due to a false positive reaction. In hyperthyroidism, an increased thyroglobulin level was observed in 64% of patients. However, there was no correlation between serum thyroglobulin and thyroxine levels in untreated hyperthyroidism. Serum thyroglobulin was increased significantly in some cases for several weeks after isotope therapy for the hyperthyroidism.

Goiter↗