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Biomedical subjects

M Yasuno

Publications and source records attributed to M Yasuno.

At least 73 records · Page 4Linked to original sources
PubMed1981

A method for assessing chronic effects of toxic substances on the midge, Paratanytarsus parthenogeneticus-effects of copper.

A method was developed for the assessment of the chronic effects of copper on midge larva. Growth and reproduction of the midge was related to copper concentrations greater than 0.08mg/L; 50% reproductive impairment was apparent at a copper level of 0.37 mg/L. Growth and amount of food uptake by larvae were inversely related to copper concentration. Accumulation of copper in green algae was proportional to copper concentration in the water.

Animals↗
PubMed1981

Novel preparation method of immunogen for hydrophobic hapten, enzyme immunoassay for daunomycin and adriamycin.

The present study was undertaken to develop a novel method of preparing a hydrophobic hapten as immunogen. The anticancer drug, daunomycin (DM) was used as prototype and coupled to mercaptosuccinylated bovine serum albumin (MS, BSA) with N-(gamma-maleimidobutyryloxy)succinimide (GMBS). Injection of rabbits with a conjugate (DM-GMBS-MS.BSA) which contained 7.3 DM per BSA molecule produced good levels of anti-DM antibody which was detected by the reaction of diluted antiserum with beta-D-galactosidase-labeled DM. beta-D-galactosidase-labeled DM was used to develop a double antibody enzyme immunoassay for DM and for the DM homologue adriamycin (AM) which reproducibly detected as little as 1 pmole of either drug. A variety of commonly used other anticancer drugs were tested and had little reactivity in this immunoassay. These studies indicate that the anti-DM serum produced is highly specific.

Animals↗
PubMed1981

Enzyme immunoassay for pepleomycin, a new bleomycin analog.

An antibody directed toward pepleomycin, a new antitumor antibiotic related structurally to bleomycin, has been produced in rabbits by immunization with a pepleomycin-protein conjugate which was prepared by a novel procedure of coupling pepleomycin to mercaptosuccinylated bovine serum albumin using N-(gamma-maleimidobutyryloxy)succinimide as a coupling agent. The antiserum was monospecific to pepleomycin and showed almost no cross-reactivity with a variety of other bleomycin analogs. An enzyme immunoassay for pepleomycin has been developed utilizing this antiserum and beta-D-galactosidase-labeled pepleomycin. The lower limit of detection by this assay, which involves a double antibody technique for the separation of antibody-bound and free antigen, was 50 pg of pepleomycin per tube. Using this assay, drug levels were easily determined in blood and urine of rabbits following administration of pepleomycin in a single dose of 1.2 mg/kg i.v. This assay is also suitable for measuring pepleomycin in the presence of other drugs since the assay is not significantly affected by any other antineoplastic agents tested. Since pepleomycin is now undergoing clinical trial, the enzyme immunoassay of the drug will be a valuable tool in clinical pharmacological studies.

Animals↗
PubMed1973

Influence of seasonal changes in climate on dispersal of released Culex pipiens fatigans: a study in villages in Delhi Union Territory, India.

Studies on Culex pipiens fatigans dispersal were conducted during the hot, cold, rainy, and post-rainy seasons in 2 villages in the Delhi area in order to improve techniques and to determine the optimum time of release. Releases from one central point and from a number of points were evaluated. No directional effect on dispersal was observed. Dispersal was most rapid and extensive in the hot season and slowest and most limited during the cold season, when the released mosquitos tended to remain near the release sites. No significant difference was noticed between male and female dispersal, and both sexes showed a uniform dispersal pattern 2 days after release.

Animals↗
PubMed1973

Dispersal and survival in the field of chemosterilized, irradiated, and cytoplasmically incompatible male Culex pipiens fatigans.

Comparative field studies were made on the dispersal and survival of chemosterilized, irradiated, and cytoplasmically incompatible (D3 strain) male Culex pipiens fatigans. There was no significant difference in dispersal patterns and daily survival rates between laboratory colony males and wild males, and between chemosterilized or irradiated males and untreated males. Although there was no difference in dispersal between adults of the D3 strain and the laboratory colony of the Delhi strain, the former had a lower daily survival rate.

Animals↗
PubMed1972

A device for the self-marking of mosquitos.

The authors describe a simple device for marking emergent adult mosquitos with fluorescent dye. This technique is of value in the study of the dynamics of insect populations.

Animals↗
PubMed1972

Studies on the life budget of Aedes aegypti in Wat Samphaya, Bangkok, Thailand.

For a complete understanding of the epidemiology of a vector-borne disease, a knowledge of the bionomics of the vector is needed. The development of Aedes aegypti was studied in Wat Samphaya, Bangkok, Thailand, where work on the adult biology had been carried out the previous year (1966-67). Particular attention was given to the variation in the numbers of immature stages of the mosquito in relation to the known seasonal incidence of dengue haemorrhagic fever. Of the three types of water container in the Wat, water jars were the main source of adults, flower pot plates were less important, and the contribution of ant traps was insignificant.The variation in the numbers of emerging adults depended on changes in the mortality of the immature stages rather than on variations in the numbers of eggs laid. Both early and late larval instar mortalities are important, the former becoming more significant during the period March-August. The mortality between the eggs and second-instar larvae is density-dependent. There was no clear trend of association between mortality and season except for a fall in larval mortality in April-May preceding the increase in annual incidence of haemorrhagic fever, which usually occurs in June.

Aedes↗