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Biomedical subjects

M Yasuda

Publications and source records attributed to M Yasuda.

At least 541 records · Page 30Linked to original sources
PubMed1980

Bile acids and bile alcohols of bullfrog.

Gallbladder bile of the bullfrog, Rana cetesbeiana was found to contain a number of minor bile acids and bile alcohols in unconjugated forms. The following bile acids and bile alcohols were identified by combined gas-liquid chromatography-mass spectrometry: cholic acid, allocholic acid, 3 alpha,7 alpha,12 alpha-trihydroxy-5 alpha-cholestan-26-oic acid, 3 alpha,7 alpha,12 alpha-trihydroxy-24-methyl-5 beta-cholestan-26-oic acid, 3 alpha,7 alpha,12 alpha,24-tetrahydroxy-5 alpha-cholestan-26-oic acid, 3 alpha,7 alpha,12 alpha,24-tetrahydroxy-5 beta-cholestan-26-oic acid, 3 alpha,7 alpha,12 alpha,26-tetrahydroxy-5 alpha-cholestan-27-oic acid, 3 alpha,7 alpha,12 alpha,26-tetrahydroxy-5 beta-cholestan-27-oic acid, 3 alpha,7 alpha,12 alpha-trihydroxy-27-nor-5 alpha-cholestan-24-one, and 3 alpha,7 alpha,12 alpha-trihydroxy-27-nor-5 beta-cholestan-24-one. These minor constituents of the bile may represent intermediates in the biosynthetic pathway of bile acids and bile alcohols in the bullfrog.

Animals↗
PubMed1979

Fine structure of the ependymal cells in the area postrema of the domestic fowl.

The ultrastructure of the ependymal cells in the area postrema of the domestic fowl was studied by scanning and transmission electron microscopy. The ependymal surface of the area postrema is covered with many furrows and ridges. These ridges consist of ependymal cells aggregated in a fan-like shape. The ependymal cell lacks clustered cilia, microvilli are few, and a long basal process extends through the parenchymal layer of the area postrema. Within the cytoplasm as well as in the basal process, a spherical body with a diameter ranging from 1.5 to 2 micron is occasionally observed.

Animals↗
PubMed1979

Scanning electron microscopy of the ventricular surface of the paraventricular organ in the domestic fowl.

The ventricular surface of the paraventricular organ in the domestic fowl is covered with supraependymal fiber elements. These fibers from a dense network having a web-like appearance and a coarse network of fibers running over the ventricular surface. The dense, web-like networks are mainly distributed throughout the caudal region of this organ, whereas the course networks are observed in the rostral region. In addition to these structures, spherical bodies with diameters approximately 2.5 to 6 micron are also encountered.

Animals↗
PubMed1979

Scanning electron microscopic analysis of the linings of the fourth ventricle in the domestic fowl.

Surface features of the ependymal linings of the fourth ventricle in the fowl were analyzed employing the scanning electron microscope (SEM). On the floor of the median sulcus, each ependymal cell has a solitary cilium, whereas on both sides of the sulcus, cilia are so densely distributed that the details of the underlying cell surface are usually obscured. On the roof of the fourth ventricle, except for the surface of the ciliated groove where numerous cilia are present, the ependymal cells are polygonal in shape, and the center of each cell possesses an aggregate of ten to twenty cilia. Cell surfaces of the choroid tela are entirely covered with delicate microvilli and possess clumped cilia. The ependymal cell surfaces of the area postrema are dome-like in shape. Each ependymal cell has a solitary cilium and shows a smooth surface free of microvilli.

Animals↗
PubMed1979

Isolation of intramitochondrial bodies in bovine adrenocortical cells by density gradient centrifugation.

Two method for isolating the intramitochondrial bodies from bovine adrenocortical cells are proposed. Electron microscopic examination shows that discontinuous sucrose density gradient centrifugation can separate the fraction rich in intramitochondrial bodies, but some indistinguishable fragments remain among them. Continuous sucrose density gradient centrifugation is probably superior to the former method in obtaining a highly purified fraction of the bodies. The amido black positive granules, presumed to be intramitochondrial bodies, are collected in the fractions of the sucrose density of around 1.27 (1.23--1.30), which lack cytochrome c oxidase activity.

Adrenal Cortex↗
PubMed1979

An electron microscopic study of periderm cell development in mouse limb buds.

Development of periderm cells covering fore- and hindlimb buds of mouse embryos was observed by scanning and transmission electron microscopy at half day intervals from day 9.5 to 12.5 of gestation (vaginal plug = day 0). At day 9.5, the epidermis is single layered. Occasional periderm cells are present at day 10.5. By day 11.5 a complete layer of periderm cells has covered the entire limb bud. By scanning electron microscopic observation, periderm cells covering the apical ectodermal ridge (AER) are characterized by a small surface size and an elongated polygonal shape with the long axis parallel to the antero-posterior contour of the apical rim. Periderm cells covering the dorsal and ventral surfaces of the limb bud are relatively large and have a polygonal surface shape. The periderm covering the apical tip reflects well the developmental state of the AER. Hence, it is possible to estimate the development of the AER by observing the surface features of the apical periderm by scanning electron microscopy.

Animals↗
PubMed1979

An improved transbuccal approach for hypophysectomy in the domestic fowl.

The present paper describes an improved transbuccal approach for hypophysectomy in the domestic fowl. With this technique, the operative procedures are the same as in the transbuccal approach of Hill and Parkes (1934), except that the drilling angle is the same as in the oral approach of Rothchild (1948a,b), since the present anatomical investigation indicates that the hypophysis lies at an angle of 45 degrees to the surface of the palate. An apparatus that was made to restrain the fowl head facilitates the operative procedure; even a novice can perform fowl hypophysectomy in more than 70% of the cases.

Animals↗