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Biomedical subjects

M Yanabu

Publications and source records attributed to M Yanabu.

46 records · Page 3Linked to original sources
PubMed1990

[Chronic ITP with a remarkable response to vitamin C administration after splenectomy].

This report deals with a case of 23-year-old woman with chronic idiopathic thrombocytopenic purpura (ITP) showing a favorable response to vitamin C (VC) administration. She was treated with glucocorticoids and immunosuppressants, whereas inadequately controlled with its tolerable dosage. Next, she underwent splenectomy. However, there were only transient effects on platelet counts, so the therapy with ascorbate was carried out according to the report by Brox et al. The platelet counts increased to 20 x 10(4)/microliters after 4 months. Side effects of VC therapy did not occur.

Adult↗
PubMed1989

[Effects of upper incisor separation on the submandibular and sublingual glands of young rats].

The effects of upper incisor separation, chronic isoproterenol (IPR) treatment and a combination with both treatments on the submandibular and sublingual glands of rats were examined and compared biochemically, immunohistochemically and radioimmunologically during 7 days after treatment. The findings obtained were as follows: 1. Both submandibular and sublingual glands of rats were significantly enlarged by an orthodontic appliance and chronic IPR treatment, and in combination with both treatments they were much more markedly enlarged additively during 7 days of treatment. This enlargement was followed, in general, by a significant increase of both RNA and DNA contents, and the incorporation of BrdU into the glandular cells, especially acinar cells, with some evidence of hyperplasia and hypertrophy. 2. Enlargement of the submandibular glands induced by orthodontic treatment was mediated via superior cervical ganglion, chorda tympani and lingual nerves, whereas of the sublingual glands mainly via chorda tympani and lingual nerves with some evidence of the mutual interactions among these nerves. 3. The secretory functions of the submandibular glands were significantly reduced at early stages after orthodontic treatment in contrast to those of chronic IPR treatment with and without orthodontic treatment. 4. The synthesis of LM protein, mainly localized in the acinar cells, was increased by orthodontic treatment in combination with adrenergic blockers, prazosin, metoprolol and ICI 118551, whereas in chronic IPR treatment it was significantly reduced by metoprolol, but not by prazosin and ICI 118551. LM protein was secreted into saliva at 6 h after IPR treatment.

Animals↗
PubMed1989

[Statistical investigation on orthodontic patients who received treatment at Fukuoka Dental College during 15 years].

It has been fifteen years since the Department of Orthodontics started in 1973, and this report concerns mainly the results of clinical observations during these period. The results were as follows: 1) Three thousand four hundred and three patients visited during a fifteen years period and 2,065 (61%) of them received orthodontic treatment. This statistical investigation was performed on 2,065 patients. 2) The numbers of treated patients increased from 1973 to 1983 without 1980 but have almost unchanged with about 180 patients from 1984 to 1987. 3) The male patients were less than the female by the ratio of 1:1.67. As to age, the patients from 6 to 12 years old accounted for 72% of all the treated. 4) Analyzed by dental age categories, patients at the stage of III A and III B comprised 54% of all the treated. 5) Analyzed by month, the patients visited on March, July and August in spring and summer vacations. 6) Most of the patients (92%) lived in Fukuoka Prefecture and 38% of them lived in Sawara-ku, where lay in the south-western part of Fukuoka city. 7) Fifty six per cent of all patients were introduced by doctors and dentists. 8) As to malocclutions, the number of mandibular protrusion was the largest, crowding case was next, and maxillary protrusion was third. The total sum of these three cases accounted for 78% of all the treated. 9) In the initial orthodontic appliances, the use of the multi-brackets appliances was first, the chin cap appliances was second and plate appliances was third. The total sum of these three appliances accounted for about 73%. 10) Analyzing to age and initial orthodontic appliances, plate, chin cap, F. K. O. and I. M. A. appliances were used at the age from 7 to 8. Lingual arch appliances were also used at the age of 8. The multi-brackets appliances increased remarkably during the recent five year period and were used at the age of 10 to 11 and over 20 years.

Adolescent↗
PubMed1989

[Analysis of platelet surface conformation in thrombin-induced aggregation].

We used flow cytometry to investigate the change of platelet membrane glycoproteins (GPIb and GP IIb/IIIa) and the distributions of fibrinogen (Fbg), thrombospondin (TSP) and fibronectin (Fn) on the surface of thrombin-stimulated platelets. The binding of a monoclonal antibody directed at the von Willebrand factor binding site on GPIb decreased in thrombin-stimulated platelets. This antibody caused a reactive delay in thrombin-induced aggregation, but had little influence on aggregability. Slight thrombin-induced aggregation was observed even after blocking the binding of Fbg to GP II b/IIIa. The new expression of GP II b/IIIa was detected on the surface of thrombin-stimulated platelets, whereas there was little increase of Fbg dependent on this GP II b/IIIa. An increase of TSP after thrombin stimulation was observed on the surface of platelets of healthy controls and patients with Glanzmann's thrombasthenia (Type I). The level of on platelet surface was slightly increased by thrombin stimulation. The mechanism involved in thrombin-induced aggregation appears to differ from that in ADP-induced aggregation.

Adult↗
PubMed1989

[Analysis of anti-platelet antibody and platelet volume in idiopathic thrombocytopenic purpura].

We investigated platelets and plasma from patients with idiopathic thrombocytopenic purpura (ITP) to elucidate the antigenic determinants at which their autoantibodies are directed, and studied the relationship between anti-platelet antibody and platelet volume. We used flow cytometry to detect platelet-associated IgG (PAIgG), C3 (PAC3), IgM (PAIgM) and platelet volume, and also to determine the binding rate of monoclonal anti-platelet antibodies in patients with ITP. The following results were obtained. 1. Both anti-GPIIb/IIIa autoantibodies (21 of 71 patients) and anti-GPIb autoantibodies (3 of 71 patients) were found in ITP. 2. The decrease in platelet count in patients without anti-GPIIb/IIIa autoantibodies was significant. 3. The increase in platelet volume was found more frequently in patients with a platelet count less than 50,000 and in untreated patients. 4. There was a positive correlation between the platelet volume and PAIgM in patients with a platelet count less than 30,000 and high levels of PAIgM.

Adolescent↗
PubMed1988

The effects of upper incisor separation on the submandibular and sublingual glands of rats.

The effects of upper incisor separation on the submandibular and sublingual glands of rats were examined biochemically, immunohistologically, and radio-immunologically during 28 days of treatment. Lateral separation of the upper incisors by application of force from an orthodontic appliance caused significant enlargement of the sublingual and submandibular glands of rats by three and seven days, respectively, after the beginning of the orthodontic treatment. This enlargement was followed by a significant increase of both RNA and DNA content, with some evidence of hyperplasia and hypertrophy. The enlargement was also associated with a significant increase of substance P at early stages after treatment, suggesting the involvement of the sensory nerves. These changes were largely inhibited by phenoxybenzamine, an alpha-adrenergic blocker, but not by atropine or morphine. Wet weights and RNA contents of the sublingual glands were markedly reduced by atropine. In comparison with control animals, the enlarged submandibular glands of rats subjected to orthodontic treatment secreted additional proteins identical with those secreted by glands enlarged by chronic administration of isoproterenol. In addition, chemical sympathectomy with 6-hydroxydopamine and phenoxybenzamine stimulated the synthesis of these abnormal proteins, but atropine and morphine did not. In contrast, protease activities in the convoluted granular tubule cells in the submandibular glands were increasingly reduced after treatment, as seen in rats subjected to chronic treatment with isoproterenol. However, the submandibular and sublingual glands completely recovered after removal of the orthodontic appliance.

Animals↗
PubMed1987

Developmental changes in the volumes, protein, and some electrolyte concentrations of male and female rat submandibular saliva secreted in response to methoxamine and pilocarpine.

Saliva secreted in response to methoxamine and pilocarpine was collected from the cannulated ducts of the submandibular glands of male and female rats at weekly intervals from two to 10 weeks of age. It was analyzed for volume and for concentrations of protein, potassium, calcium, and inorganic phosphate. Following the collection of saliva, the submandibular glands were removed and weighted. The wet weights of the glands increased substantially up to seven weeks of age and then reached almost plateau values in both sexes. The salivary volumes secreted in response to both agents in both sexes were positively correlated with the gland weights, except that after five to six weeks of age there was no correlation between gland weight and methoxamine-stimulated salivary volume. The concentrations of protein, potassium, and inorganic phosphate were inversely related to the flow rates only at relatively low rates of flow. The concentration of calcium was positively correlated with the protein concentration and was independent of the nature of the stimulus and of sex differences during postnatal development.

Animals↗
PubMedDate not supplied

Characterization of a novel activation-specific antiplatelet monoclonal antibody.

We developed a murine monoclonal antibody (KmT-50) which binds to a protein expressed on the surface of human platelets after activation. KmT-50 is an immunoglobulin G1 monoclonal antibody that recognizes a 50-kD antigen localized in intracellular organelles and the open canalicular system of unstimulated platelets. After platelets were stimulated, the antigen was secreted via the surface-connected canalicular system and exposed on the platelet membrane. KmT-50 bound not only to human platelets, but also to rabbit and monkey platelets. This new antibody may be useful for experimental studies on thrombosis and hemostasis.

Animals↗
PubMedDate not supplied

Platelet-derived microparticles in alloxan-induced diabetes in rabbits.

To study platelet-derived microparticle generation in diabetes mellitus, we injected alloxan into male Japanese white rabbits. Injection of alloxan induced diabetes, but did not cause any significant change in various biochemical and hematological parameters. However, diabetic rabbits showed a significant elevation of platelet-derived microparticles from 8 weeks after alloxan injection (week 0: 0.45 +/- 0.24%; week 8: 1.12 +/- 0.61%, p < 0.005). These microparticles are known to have prothrombinase activity, suggesting that they may promote vascular complications in diabetes and may be used as a marker of vascular disease.

Alloxan↗