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Biomedical subjects

M Yakata

Publications and source records attributed to M Yakata.

At least 55 records · Page 3Linked to original sources
PubMed1986

Monoclonal autoantibodies in Heymann nephritis.

We have developed hybridoma cell lines, each of which secretes a monoclonal antibody (MoAb) to rat renal and hepatic tissue antigens, from Lewis rat with Heymann's nephritis. Three antibodies bind to the brush border of proximal tubular epithelium (BB), one in a fine net-like pattern (no. 3-11), another one in a coarse granular pattern (no. 1-8) and the third one in a typical pattern (no. 3-9). Three antibodies bind to glomerulus in characteristic patterns but not to BB. After repeated intravenous injections of MoAb (no. 3-11), granular mesangial deposits of rat IgG were observed and of MoAb (no. 1-8), fine granular deposition along capillary walls. These monoclonal autoantibodies should be of value in research on the mechanism of autoimmune membranous nephropathy.

Animals↗
PubMed1985

Age- and sex-related differences in urinary excretion of 6 beta-hydroxycortisol in humans.

A study of normal subjects showed that the 24-h urinary 6 beta-hydroxycortisol level is age-dependent, with a maximum at 14-20 yr in both men and women. The ratio of 6 beta-hydroxycortisol/cortisol also showed an age-dependent variation, being higher in children than adults. There was a significant difference between boys and girls aged 5-10 yr, but no difference in other subjects. No sex difference was demonstrable in the ratio of 6 beta-hydroxycortisol/cortisol. Examination of circadian rhythm revealed that 6 beta-hydroxycortisol and cortisol correlated well in children, but showed poor correlation in adults, with a lowered morning peak of 6 beta-hydroxycortisol.

Adolescent↗
PubMed1985

Determination of urinary cortisol and 6 beta-hydroxycortisol by high performance liquid chromatography.

A method using high performance liquid chromatography for the quantitative determination of cortisol and 6 beta-hydroxycortisol in urine is described. Urine extracts were fractionated by high performance liquid chromatography. The peak of 6 beta-hydroxycortisol was identified on the basis of retention time (24.4 min) and its area was measured. The peak of cortisol could not be measured simultaneously with the same column because of interference by other absorbing materials. The cortisol and dexamethasone peaks were collected (Rt values 9.6 and 8.1 min, respectively), rechromatographed on another column system, and the concentration was determined. Precision and accuracy of the present method were within the range commonly achieved by other methods established for both steroids. Clinical utility of the present method was evaluated by measuring urinary cortisol and 6 beta-hydroxycortisol in normal subjects, in Cushing's syndrome and disease patients, and in patients receiving cortisol therapy.

17-Hydroxycorticosteroids↗
PubMed1985

[Comparison of in vitro antibacterial activities of new cephems against clinical organisms (isolated between June 1983 and January 1984)].

We compared the in vitro antibacterial activities of ceftizoxime (CZX), cefotaxime (CTX), cefmenoxime (CMX), cefoperazone (CPZ), ceftazidime (CAZ), latamoxef (LMOX) and cefotetan (CTT) against 2,729 strains of 11 organisms freshly isolated from 10 medical institutions in Japan between June 1983 and January 1984 and obtained the following results: Against S. pyogenes, LMOX and CTT, which have the methoxy group at the 7 position, were less active than the other drugs. LMOX inhibited 80% of S. pyogenes at 0.78 micrograms/ml; CTT, at 1.56 micrograms/ml; but CZX and CTX inhibited 100% at 0.025 micrograms/ml or lower; CMX, at 0.05 micrograms/ml; and CPZ and CAZ, at 0.20 micrograms/ml. Against H. influenzae, E. coli, K. pneumoniae, P. mirabilis and indole-positive Proteus, these test antibiotics, especially CZX, CTX and CMX, which have the aminothiazolyl methoxyimino group, were potently active. Against S. marcescens CZX and CAZ were more active than the other drugs and against P. aeruginosa CAZ was more active than the other drugs. The test organisms did not tend to acquire resistance to these cephems when our results were compared with the results obtained at the development period.

Bacteria↗
PubMed1984

Age- and sex-related differences in urinary cortisol level.

A comparative study between normal groups showed that the 24-h urinary cortisol level is age-dependent both in men and women. The comparison between men and women with age-matched groups revealed a significant difference in the lower age groups up to 20-24 years. When urinary cortisol values were corrected for creatinine excretion, the age-related variation was eliminated to some extent, but not completely. The correction could correct the sex-related difference in the lower age groups, but it brought about another sex-related difference in the advanced age groups. There was no remarkable age- or sex-related difference in the circadian rhythm.

Adolescent↗
PubMed1984

Portable file management system in FORTRAN.

It has been generally considered that FORTRAN is inferior to MUMPS and other computer languages in its facility to manipulate files and that it is not satisfactorily competent to handle databases. However, it is our finding that FORTRAN is the most widely used language in patient data management systems discussed in recently published papers. Therefore, it now seems appropriate to review and evaluate the validity of this general belief. The objective of this study is to establish a file management system in FORTRAN 77 for use in the construction of a clinical database. Comparative study is conducted on several problems associated with file manipulation, that is, time requirement for file access and access methods for data retrieval.

Computers↗