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Biomedical subjects

M Wu

Publications and source records attributed to M Wu.

At least 379 records · Page 21Linked to original sources
PubMed1995

[The effects of combined hepatectomy and immuno-chemotherapy on postoperative recurrence rate of primary liver cancer].

121 cases with primary liver cancer were divided into four groups: OP: resection only (30 cases); OC: combined operative resection and chemotherapy (27 cases); OI: combined operative resection and immunotherapy (31 cases); OI: combined operative resection and immuno-chemotherapy (33 cases). The results showed that one year recurrence rate was 56.7%, 40.7%, 32.3% and 27.3%, respectively. The recurrence rate of group OI and group OIC were significantly lower than that of group OP and group OC. It indicated that combined operation and immuno-chemotherapy is useful in preventing post operative recurrence.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1995

Clinical value of urinary and serum pseudouridine in diagnosis and monitoring of primary liver cancer.

Urinary and serum pseudouridine concentrations were determined by high-performance liquid chromatography in 80 patients with primary liver cancer, 32 with benign space occupying lesions of the liver, 42 with liver cirrhosis and 40 healthy subjects. Their mean urinary and serum pseudouridine levels were 39.2 +/- 11.5 nmol/mumol creatinine and 3.4 +/- 1.3 mumol/L, 24.5 +/- 5.4 nmol/mumol creatinine and 2.5 +/- 0.5 mumol/L, 22.8 +/- 7.8 nmol/mumol creatinine and 2.3 +/- 0.4 mumol/L, 26.4 +/- 4.6 nmol/mumol creatinine and 2.3 +/- 0.4 mumol/L, respectively. Exceeding the mean plus 2SD of pseudouridine of healthy control was considered as positive value for the diagnosis of primary liver cancer. Thus the positivity of urinary and serum pseudouridine in hepatoma was 71.3% and 70.0%, respectively. The positive rate of combined pseudouridine and alpha-fetoprotein assay was 91.3% in patients with hepatoma. Besides, pseudouridine levels could elevate before positive localization and reduce to normal levels after tumor resection. The results showed that the determination of pseudouridine is of clinical significance in the diagnosis and monitoring of primary liver cancer.

Biomarkers, Tumor↗
PubMed1995

Nutritional intervention to prevent hereditary cancer.

To determine if the effect of nutritional interventions differs by genetic susceptibility to cancer, we must have both an effective intervention as well as a documented marker of genetic susceptibility. The first large clinical trials to test nutritional intervention strategies have recently been reported, and apparent efficacy has been observed for selected antioxidants in the primary prevention of several cancers, including esophageal, stomach, prostate, and colorectal cancers. At the same time, increasing numbers of markers of genetic susceptibility are being identified. Although susceptibility markers have not yet been evaluated in the context of nutritional interventions in humans, preliminary data in animals indicate that calorie restriction reduces spontaneous tumor mortality in p53-knockout mice. Linking the results from nutritional interventions in humans with markers of genetic susceptibility will allow us to better understand gene-environment interactions.

Animals↗
PubMed1995

Treatment of 23 patients with advanced gastric cancer by intravenously transfer of autologous tumor-infiltrating lymphocytes combined with rIL-2.

Tumor-infiltrating lymphocytes (TIL) isolated from metastatic lymph nodes in patients with nonoperable advanced gastric cancer were induced to become LAK-like cytotoxic activity of TIL after in vitro culture with rIL-2. Twenty-three patients with advanced gastric cancer were treated by intravenously transfer of autologous TIL combined with rIL-2. The tumor focus disappeared (complete remission, CR) in 3 patients (13.0%) and significantly decreased (partial remission, PR) in 5 patients (21.7%). Fifteen patients did not respond to the treatment. The amount of soluable IL-2 receptor in serum was significantly decreased after treatment, the cytotoxicity of NK cells and OT test were significantly increased. No significant difference in CD4/CD8 was found between before and after treatment. No serious side effect was observed in the treatment.

Adenocarcinoma↗
PubMed1995

[Effects of some physical and chemical factors on both human hair DNA and results of sex determination by PCR].

The present study aims at observation of the effects of some physical and chemical factors on both human hair DNA and the results of sex determination by PCR. After the hairs were treated with varying pH solutions, the quantity of the DNA extracted was decreased. High-molecular-weight (HMW) DNA was determined in the hairs treated with pH 1, 3, 5 and 7 solutions for 2 hours, but no DNA was demonstrated by agarose gel electrophoresis in the hairs treated with pH 1 and 3 solution for 24 hours, and the quantity of DNA was decreased in the hairs treated with pH 5 for 24 hours. The quantity of DNA was decreased in the hairs treated with pH 9 for 2 hours and no DNA was demonstrated for 24 hours. No DNA was found in the hairs treated with pH 11 for 2 or 24 hours. No changes of DNA were observed in hairs treated with 75% ethanol or methanol for 2 or 24 hours and exposed to UV light for 24 hours. No DNA but RNA was observed in the hairs treated with 10% formalin (pH 7 or pH 5) for 2 or 24 hours. The quantity of DNA was decreased obviously in the hairs treated in 100 degrees C water for 5 or 10 minutes. The quantity of DNA was not decreased in the hairs heated at 50 degrees C for 24 hours, and only little decrease was noted at 100 degrees C for 24 hours. Both fragments of PCR products were obtained in most hairs except the hairs treated with pH 11 solution for 24 hours. Although no DNA was observed in some treated hairs, specific bands were detected after PCR. PCR is a very sensitive and specific technique of DNA amplification, which can be used for sex determination in forensic medicine.

Base Sequence↗
PubMed1995

[Percutaneous ethanol injection therapy for the treatment of postoperative recurrent primary liver cancer].

This article reported 109 cases of postoperative recurrent primary liver cancer, treated with percutaneous ethanol injection therapy (PEIT) under the guidance of conventional ultrasonic transducer, the total number of injection being 637 times. No metastasis through the needle track or other serious complications were encountered. The 1.3 and 5 year survival rates were 92.6%, 47.8%, and 19%, respectively. We noted that the key factor affecting the efficacy of treatment was the accurate localization of puncture, rather than the times of injection or the quantity of ethanol injected. Criteria for the judgement of treatment and precautions to be noted concerning the procedure were suggested. It is concluded that PEIT is the treatment of choice in the management of non-operable, single, and comparativly smalle focus of postoperative recurrent primary liver cancer.

Adult↗
PubMed1995

[Amplified fragment length polymorphism of the VNTR locus COL2A1 in Chinese population].

The amplifiable VNTR polymorphic system COL2A1 has been investigated in a Chinese Han population (n = 120) by the polymerase chain reaction (PCR) and PAGE horizontal electrophoresis followed by silver stain. In order to accurately identify COL2A1 alleles, a number of human allele ladders prepared by mixing DNAs extracted from different individuals of known COL2A1 genotypes were used. A total of 14 different alleles in 23 genotypes were observed in this Chinese Han population. Among them, four were new alleles disclosed in the present study. The results imply that COL2A1 locus may be served as a genetic marker in forensic haemogenetics as well as in anthropogenetics.

Base Sequence↗
PubMed1995

[Iatrogenic bile duct injuries during the process of laparoscopic cholecystectomy].

Twelve patients with iatrogenic bile duct injuries occurred during laparoscopic cholecystectomy (LC) were treated from June 1992 to May 1994. All the patients underwent re-operation and were cured. The causes and characteristics of the injuries were: (1) perforation of the common hepatic or common bile duct caused by dissecting hook (3 cases); (2) necrosis and perforation of the common hepatic duct due to diathermic injury (1 case); (3) clamping of the common hepatic duct by Ti clip (1 case); (4) secondary high bile duct stricture following a failed end-to-end anastomosis or hepatico-cholangio-jejunostomy of the amputated common hepatic duct (5 cases); (5) delayed high bile duct stricture (2 cases). It is emphasized that the severity of bile duct injuries by LC be should not overlooked, and more experience in this field be accumulated to avoid this serious complication.

Aged↗
PubMed1994

YKE2, a yeast nuclear gene encoding a protein showing homology to mouse KE2 and containing a putative leucine-zipper motif.

The nucleotide sequence of YKE2, a yeast nuclear gene, has been determined. The deduced YKE2 protein has 114 amino acids (12 kDa), shows significant homology with the murine KE2 and contains a putative Leu-zipper motif characteristic of a group of DNA-binding proteins [Landschulz et al., Science 240 (1988) 1759-1764]. Strains in which YKE2 has been disrupted show normal cell growth in glucose and galactose media over the temperature range of 16 to 40 degrees C. Disrupted strains also display normal mating and sporulation abilities. Northern analysis revealed that the transcription of YKE2 is unresponsive to catabolite repression by glucose.

Amino Acid Sequence↗
PubMed1994

The Saccharomyces cerevisiae homologue of ribosomal protein S26.

The nucleotide sequence of RPS26, the gene encoding a homologue of ribosomal protein small subunit S26 in Saccharomyces cerevisiae, was determined. The deduced amino-acid sequence showed significant identity with its counterparts from Neurospora crassa, human, rat and Arabidopsis thaliana. Disruption of RPS26 resulted in the formation of micro-colonies, suggesting that it is important for the normal cell growth of S. cerevisiae.

Amino Acid Sequence↗
PubMed1994

The stability and structure of tandem GA mismatches in RNA depend on closing base pairs.

UV melting and imino proton NMR studies show that the stabilities and structures of tandem GA mismatches in RNA are dependent upon the closing base pairs around these mismatches. Internal loops of sequence 5'XGAY3'3'YAGXS' and 5'XAGY3'3'YGAX5' in the middle of octanucleotides have a range of stabilities over 5 kcal/mol when XY is a Watson-Crick or GU pair. The order of stabilities for these internal loops is 5'-GGAC-3' > UGAG, CGAG > AGAU > UGAA > GGAU. The motifs GGAC, UGAG, and CGAG are stabilizing, while the other GA motifs are destabilizing. The GAGC motif is more stable than CAGG and CGAG, but less stable than GGAC. Chemical shifts for imino protons suggest that the G imino proton of each GA mismatch in 5'-GGAC-3', 5'-GAGC-3', and 5'-CAGG-3' [SantaLucia, J., Jr., Kierzek, R., & Turner, D. H. (1990) Biochemistry 29, 8813-8819] is involved in a hydrogen bond to the base A, whereas in other 5'-XGAY-3' sequences, it is not involved in a hydrogen bond to the base A.

Adenine↗
PubMed1994

Transforming growth factor-beta up-regulates human elastin promoter activity in transgenic mice.

We have recently developed transgenic mice which express approximately 5.2 kb of the human elastin promoter linked to the chloramphenicol acetyl transferase (CAT) reporter gene (J. Biol. Chem. 269:18072-18075, 1994). Previously, transforming growth factor-beta (TGF-beta) has been shown to enhance elastin gene expression, as determined at the mRNA and protein levels. To examine whether this enhancement could be explained by upregulation of the elastin promoter, TGF-beta 1 (100 ng) was injected subcutaneously into the transgenic animals. CAT activity in the skin of treated animals was elevated in a time-dependent manner up to approximately 10-fold after a single injection. These results suggest that the 5.2-kb up-stream segment of the human elastin gene contains cis-elements responsive to TGF-beta 1 in vivo.

Animals↗
PubMed1994

Tissue-specific and developmentally regulated expression of human elastin promoter activity in transgenic mice.

We have recently cloned the entire human elastin gene, including approximately 5.2 kilobases of the 5'-flanking sequences. To examine tissue-specific expression of the elastin gene, we have developed a transgenic mouse line that expresses the human elastin promoter linked to the chloramphenicol acetyltransferase (CAT) reporter gene. Assay of CAT activity in different tissues revealed the highest expression in the lungs and aorta, while lower levels were detected in the kidneys, heart, brain, and skin; this distribution parallels the accumulation of elastin in developing animals. Comparison of CAT activity in the lungs of fetal (15-day gestation) and newborn (5-day postnatal) animals revealed significantly (approximately 4-fold) higher activity in the fetal tissue. The relatively high activity in the lungs progressively declined during the postnatal period up to 6 months. The promoter activity in the aorta remained constant from 5 days to 3 months and then gradually declined, while in the skin, the activity peaked at 3 months, returning thereafter to the control (5-day) level. Thus, there is evidence for developmentally regulated, tissue-specific expression of the elastin promoter in vivo as tested in these transgenic mice.

Aging↗