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Biomedical subjects

M Wu

Publications and source records attributed to M Wu.

At least 253 records · Page 14Linked to original sources
PubMed1998

[Effects of tea polyphenol on blood lipid and antioxidation in vivo in aged rats].

OBJECTIVE: To study the ability of tea polyphenol to lowering blood lipid and antioxidation in the aged rats. METHODS: The SD rats were divided into three groups, i.e., control group and two trial groups fed with 1% and 2% tea polyphenol for six weeks, respectively. RESULTS: Tea polyphenol could reduce serum level of lipid peroxide and increase the ratio of high-density lipoprotein cholesterol (HDL-C) to total cholesterol (TC), and significantly lower serum level of lipid peroxide in rats. Activities of superoxide dismutase in red blood cells of rats fed with 2% tea polyphenol were significantly higher than those in control ones. CONCLUSION: Tea polyphenol can enhance antioxidation in vivo in the aged rats.

Aging↗
PubMed1998

[A study of the adenosine deaminase polymorphism in a Chinese Han population by the PCR method].

The study of genetic polymorphism of adenosine deaminase (ADA) has not been reported in China and abroad. The target DNA was amplified by PCR. The amplified product was then digested with Pst I, and run on a 9% polyacrylamide gel electrophoresis and followed by AgNO3 staining. ADA polymorphism, was determined in 80 unrelated individuals Chinese Han polulation. Five alleles and 14 genotypes were detected. The gene frequencies were ADA * 10 = 0.15625, ADA * 11 = 0.31875, ADA * 12 = 0.23750, ADA * 13 = 0.23125 and ADA * 14 = 0.05625 respectively. The heterozygosity was 0.8 and the discrimination power (DP) was 0.89. The distribution of observed genotypes frequencies was in good agreement with the Hardy-Weinberg law. The study of three families demonstrated that this locus was inherited according to Mendelian law. The technique is quite simple, fast, extremely sensitive, and it does not require the use of radioisotopes. This new method may be used for the forensic identification and paternity testing.

Adenosine Deaminase↗
PubMed1998

[Randomized comparative study of GyneFix IN and TCu 380A intrauterine devices].

OBJECTIVE: To observe the clinical performances of the new intrauterine device (IUD)-GyneFix IN. METHODS: The present study is a randomized comparative clinical trial. 607 healthy parous women were randomly allocated into GyneFix IN group (n = 302) or TCu 380A group (n = 305). IUD was inserted during the menstrual interval by the trained investigators. Follow-up were arranged at 1, 3, 6 and 12 months after insertion. The discontinuation rates were calculated by life table method. RESULTS: At the end of the first year, there was no pregnancy occurred in GyneFix IN group. Its expulsion rate and removal rate for medical reasons were 2.67 and 1.02 per 100 women respectively. The use-related discontinuation rates was 3.66, which was significantly lower than that in the TCu 380A group (7.88, P < 0.05). The number of women with complaint of pain was also less in GyneFix IN group. CONCLUSION: The excellent performance of the new IUD which is a frameless device with high copper surface and anchoring system was confirmed by this multicentre trial. Due to lower expulsion rate and less side effect of pain, it could be recommended.

Adolescent↗
PubMed1998

[Insulin-like growth factor-I upregulates the growth stimulatory effect of epidermal growth factor on HCE16/3 cells].

OBJECTIVES: To examine the role of insulin-like growth factor-I (IGF-I) in growth and gene expression of human papillomavirus (HPV) type 16 DNA-immortalized human cervical epithelial cells (HCE16/3 cells). METHODS: The growth and gene expression of HCE16/3 cells treated with IGF-I and epidermal growth factor (EGF) were examined by flow cytometry, soft-agarose assay and RT-PCR analysis. RESULTS: Physiological concentration of IGF-I had little effect on proliferation of HCE16/3 cells in serum-free and growth factor-free medium but supraphysiological concentration of IGF-I stimulated moderate proliferation of HCE16/3 cells. EGF stimulated proliferation of HCE16/3 cells at physiological concentration. The stimulatory effect was stronger when combined with IGF-I than EGF alone. Furthermore, the combined use of EGF and IGF-I could induce colony formation of HCE16/3 cells in soft agarose. The expression of HPV 16 E7 gene in HCE16/3 cells was not altered by growth factors in RT-PCR analysis. CONCLUSION: IGF-I is a weak regulator of HCE16/3 cells but it enhances the growth stimulatory effect of EGF on HCE16/3 cells.

Cell Division↗
PubMed1998

[The effect of immunosuppression on the expression of perforin and granzyme B mRNA in hamster to rat liver transplantation].

OBJECTIVE: To explore the variation on the expression of perforin and granzyme B gene mRNA to judge the effect of immunosuppression in acute rejection of liver transplantation. METHODS: The expression of perforin and granzyme B gene mRNA was examined by RT-PCR in hamster to rat liver grafts under the immunosuppression of cyclosporine and splenectomy. Histological findings were studied comparatively. RESULTS: Cyclosporine and splenectomy could obviously suppress the rejection of liver grafts. The survival time of animals was significantly prolonged (37.1 days). The architecture of hepatic lobule was preserved. There was slight round cell infiltration in the portal tracts and no expression of mRNA of perforin and granzyme B genes could been seen in three weeks after transplantation. CONCLUSION: Perforin and granzyme B genes are of value in judging the effect of immunosuppression.

Animals↗
PubMed1998

[Fas mediated apoptosis inhibited by human bcl-2 gene in lymphoma cell line Jurkat].

OBJECTIVE: To understand the mechanism of bcl-2 gene in escaping the immune surveillance in the development of lymphoma. METHODS: A recombinant retroviral vector pLXSN-bcl-2 was constructed by cloning bcl-2 cDNA into the replication defective retroviral vector pLXSN, and transferred to packaging cell line PA317 by electroporation. The G418 resistant colonies were selected, and the supernatants of the colony cultures were used to infect the human lymphoma cell line Jurkat. Cells in G418 resistant Jurkat colonies were characterized by immunohistochemistry. Anti-Fas monoclonal antibody (McAb) was applied to Jurkat cells for inducing apoptosis which mimicked the cytotoxic activity of T lymphocyte. RESULTS: Expression of bcl-2 in pLXSN-bcl-2 transfected Jurkat cell (Jurkat-bcl-2) increased, while there was no change of Fas gene expression. Apoptosis was blocked in Jurkat-bcl-2 by anti-Fas McAb treatment. CONCLUSION: Overexpression of bcl-2 in lymphoma cell line could inhibit cell apoptosis induced by anti Fas McAb, suggesting that overexpression of bcl-2 is one of the mechanisms in escaping immune surveillance in the development of lymphoma.

Apoptosis↗
PubMed1998

[Perforin and Fas-ligand expression of tumor infiltrating lymphocytes in human hepatocellular carcinoma].

OBJECTIVE: To investigate perforin and Fas-ligand (Fas-L) expression of tumor infiltrating lymphocytes (TIL) in human hepatocellular carcinoma (HCC). METHODS: Expression of perforin and Fas-L of TIL was studied in 20 HCC cases using in situ hybridization and immunohistochemistry. RESULTS: Expression of perforin and Fas-L gene were detected in TIL in 80% of the cases studied. Among them, one patient (no. 14) in whom expression of perforin and Fas-L were noticed in the majority of the TIL, had no recurrence of HCC for one and one half (1.5) year after tumor resection. It indicates that presence of large number of activated T cells might be beneficial for the tumor therapy. In the remaining cases, only 10% of TIL were obtained able to express perforin and Fas-L indicating that only a few TIL were activated and cytotoxic to HCC. CONCLUSIONS: Even there were a multitude of T cells infiltrating in HCC, only few of them were immunoactived and to be cytotoxic to HCC. It seems important to adopt measures in order to promote further proliferation of these activated T cells either in vitro or in vivo.

Adult↗
PubMed1998

Expression of splice variants of CD44 mRNA and its significance in human primary hepatocellular carcinoma.

OBJECTIVE: To investigate the relationship between the expression of splice variants of CD44 (CD44v) and the metastasis of primary hepatocellular carcinoma (PHCC) in humans. METHODS: The expression of metastasis-associated CD44v mRNA in 54 specimens of human PHCC was detected by reverse transcription and polymerase chain reaction (RT-PCR) method. RESULTS: CD44v mRNA was expressed in 50 (92.59%) of the 54 specimens of PHCC, with moderate or over expression in 42 (77.78%). Among the 42 specimens with moderate or over expression, deficient tumor capsules were found in 25 (59.52%), penetration of tumor capsule in 15 (35.71%), tumor emboli in the portal vein in 34 (80.95%), and daughter tumor nodules in 29 (69.05%). Meanwhile, 12 of the 54 specimens showed negative or weak expression of CD44v mRNA. Of them, all had tumor capsules, 1 (8.33%) had penetrated tumor capsule, 1 (8.33%) had tumor emboli in the portal vein, and 2 (16.67%) had daughter tumor nodules. CONCLUSION: CD44v may contribute to the metastatic potential of PHCC.

Carcinoma, Hepatocellular↗
PubMed1998

[Effects of tetramethylpyrazine and radix salviae miltiorrhizae on collagen synthesis and proliferation of cardiac fibroblasts].

OBJECTIVE: To explore the effects of Tetramethylpyrazine (TMP) and Radix Salviae Miltiorrhizae (RSM) in collagen synthesis and proliferation of cardiac fibroblasts. METHODS: Using collagenase and trypsase digested rat cardiac tissue assay to isolate cardiac fibroblasts (Fbs). Different dosage of TMP, RSM and norepinephrine (NE) were used to study their effects on the collagen synthesis and proliferation of cultured cardiac Fbs. RESULTS: Compared with the control group, moderate or high dosage TMP and RSM could significantly inhibit the collagen synthesis and the proliferation of cultured cardiac Fbs. Moreover, low-dose TMP(50 mg/L) and low-dose RSM(3 g/L) could antagonize the collagen synthesis and the proliferation of cultured cardiac FB stimulated by NE (500 micrograms/L). CONCLUSION: Both TMP and RSM could inhibit these processes. The mechanisms of these effect might be correlated to their Ca++ antagonistic action.

Animals↗
PubMed1998

[The detection of circulating hepatocellular carcinoma cells in peripheral venous blood by reverse transcription-polymerase chain reaction and its clinical significance].

OBJECTIVE: To detect circulating hepatocellular carcinoma by demonstrating hepatocellular carcinoma cells-associated mRNA in the nuclear cell component of peripheral blood (PBL). METHOD: Peripheral blood (5 ml) samples were obtained from 93 patients with hepatocellular carcinoma (HCC) and from 37 controls (15 controls with liver cirrhosis after hepatitis B, 12 chronic hepatitis B, and 10 normal liver function). To identify HCC cells in peripheral blood, liver-specific human alpha-fetoprotein (AFP) mRNA was amplified from total RNA extracted from whole blood by reverse transcription-polymerase chain reaction. RESULT: AFP mRNA was detected in 50 blood samples from the patients with HCC (53.8%). In contrast, there were no clinical control patients whose samples showed detectable AFP mRNA in PBL. The presence of AFP mRNA in blood seemed to be correlated with the stage (by TNM classification) of HCC, the serum AFP value, and the presence of intrahepatic metastasis, portal vein thrombosis, tumor diameter and/or distant metastasis. AFP mRNA was detected in the blood of 21 patients showing metastasis at extrahepatic organs (100%) in contrast to 29 of 72 patients without metastasis (40.3%). CONCLUSION: The presence of AFP mRNA in peripheral blood may be an indicator of malignant hepatocytes, which might predict hematogenous spreading metastasis of tumor cells in patients with HCC.

Adult↗
PubMed1998

[The history of heroin abuse by assaying 6-monoacetylmorphine and morphine in human hair].

The purpose of this study is to conclude the history of heroin abuse by assaying 6-monoacetylmorphine (MAM) and morphine (MOR) in human hair. The hair of heroin abuse was labeled and segmented, then washed and cut into fragments. After hydrolyses and extraction, 6-MAM and MOR in human hair were determined by GC/MS-SIM with selected ion monitoring. Results of the segmented hair were analyzed. It provided useful information about the history of heroin abuse (hair growth rate 1-1.5 cm/mon).

Adult↗
PubMed1998

[A program of HLA system for parentage testing and its application].

A software of the HLA blood group system for parentage testing was developed with FoxPro 2.5 system. Chinese prompts helped to follow the process during program running. It is easy to use. 63 cases of parentage testing in our department were calculated, and the results were identical with those of manual calculation. It was believed that this program is useful in the parentage testing.

Blood Group Antigens↗
PubMed1998

[Detection of heroin metabolites: 6-monoacetylmorphine and morphine in human hair by GC/MS].

This paper presents a method to detect the main metabolites of heroin: 6-monoacetylmorphine (6-MAM) and morphine (MOR) in human hair using GC/MS-SIM. The hair specimens were washed with special solvents and cut into about 0.5 mm pieces. Ethylmorphine was added as internal standard and HCl solution for hydrolysis. After hydrolysis, 6-MAM and MOR were extracted by a mixture of solvents (chloroform-isopropyl alcohol-heptane 50:17:33). The residue of the extract was derivatized with N-methyl-N-trimethylsilyl trifluoroacetamide(MSTFA), then the trimethylsilyl(TMS)-derivatives were qualitatively and quantitatively analyzed using GC/MS-SIM. The correlation coefficients for 6-MAM and MOR were 0.9996 and 0.9997, respectively. The recoveries of both 6-MAM and MOR were over 50%. The RSD of within-day and between-day was less than 8% and 10%, respectively. The lower limit of detection of both 6-MAM and MOR was 0.5 ng.mg-1. Hair samples of 12 drug abusers were analyzed using this method, 8 of them gave positive results. This method is simple, accurate and sensitive. It is very suitable for routine case work.

Gas Chromatography-Mass Spectrometry↗
PubMed1998

[Research advances on fusion peptide and mechanisms about virus penetration into membrane].

The first step of viral infection is virus fusion with target membrane. The process is induced by fusion peptide located in glycoprotein on virus membrane. Fusion peptide exploited after interaction with receptors and penetrates into target cells membrane. Lipid molecules surrounding peptides are rearranged and membrane fusion occurs through the formation of a intermediate state. This review introduces the research advances of viral fusion peptides and its mechanism of penetrating into membrane.

Animals↗
PubMed1998

[Preparation of liposome encapsulated daunorbicine and determination of daunorubicine of aqueous humor in rabbit eyes].

PURPOSE: To investigate the method of determination the concentration of daunorubicine(DNR) and liposome encapsulated daunorubicine(LDNR) of aqueous humor in rabbit eyes. METHODS: After extracapsular lens extraction, 0.1 ml of 1 mg/ml DNA and LDNR were injected into the anterior chamber. At the 12, 24, 48 hours and 1 week after operation, the aqueous humor were aspirated to determine the concentration of DNR by high performance liquid chromatography(HPLC). RESULTS: At the 12, 24, 48 hours after operation, DNR were determined in all eyes, but at one week after operation, the DNR could only be detected in LDNR group. The concentration of DNR in LDNR group were higher than DNR group.

Animals↗