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M Wootton

Publications and source records attributed to M Wootton.

47 records · Page 3Linked to original sources
PubMed1993

Simple method for production of internal control DNA for Mycobacterium tuberculosis polymerase chain reaction assays.

A simple method for the production of internal control DNA for two well-established Mycobacterium tuberculosis polymerase chain reaction assays is described. The internal controls were produced from Mycobacterium kansasii DNA with the same primers but at a lower annealing temperature than that used in the standard assays. In both assays, therefore, the internal control DNA has the same primer-binding sequences at the target DNA. One-microgram quantities of internal control DNA which was not contaminated with target DNA could easily be produced by this method. The inclusion of the internal control in the reaction mixture did not affect the efficiency of amplification of the target DNA. The method is simple and rapid and should be adaptable to most M. tuberculosis polymerase chain reaction assays.

DNA, Bacterial↗
PubMed1993

Stevie.

Explore the source record for details and available documents.

Dysgerminoma↗
PubMed1991

The role of restraint and disinhibition in appetite control.

Fifty-six women classified according to their restraint and disinhibition scores, using Stunkard and Messick's (1985) Eating Questionnaire, participated in an experiment in which the proportion of carbohydrate in a prepared meal and the knowledge provided to them about its contents were varied. Measures were taken of their desire for food and their willingness to eat, as well as the total intake and the amount of carbohydrate and of protein they ate at an ad libitum test meal 4 hr later. While the results do not support the argument that laboratory-induced counter-regulatory eating can be attributed to disinhibition, they emphasize the usefulness of our experimental model which allows the interaction of dietary, cognitive and personality factors to be examined concurrently in investigations of appetite control.

Adult↗
PubMed1987

Towards the prevention of eating disorders. Some preliminary findings from an empirical investigation.

The high incidence of disordered eating among female adolescents and young adult women has been noted in surveys carried out in Australia, the USA, New Zealand and the UK. While a critical feature that distinguishes those who go on to develop anorexia nervosa or bulimia nervosa is their sense of a loss of control over their eating, the psychological and nutritional concomitants of this loss of control have not been empirically investigated. Preliminary findings are described from an experimental study which is aimed at systematically investigating the relationship between dietary composition and consumatory patterns, hunger and satiety. The results are discussed in terms of the possible role of 'diet' in perpetuating the symptoms of bulimia nervosa and anorexia nervosa.

Adolescent↗
PubMed1986

Urine mutagenicity as an indicator of exposure to dietary mutagens formed during cooking of foods.

Studies were undertaken with individuals fed fried bacon meals to determine whether fruit or vegetables, ingested along with bacon, modified uptake and subsequent excretion of bacon mutagen(s). Urinary mutagenic activity was significant in those who had consumed bacon or mixed bacon/vegetable or bacon/fruit meals within the previous 2 to 3 hr period. Although urine activity varied by a factor of 4 among 15 subjects who consumed different meals, there was no evidence from this investigation that fruit or vegetables contributed to the inherent variability in total urinary mutagenic activity. However, some differences in excretion kinetics may be attributable to vegetable or fruit supplements in mixed meals.

Animals↗
PubMed1983

Mutagenic activity of heated potato/oil systems.

Mutagens detected with Salmonella typhimurium strain TA 98 in the presence of liver S9 mix were extracted from potato slices, but not pure potato starch, after frying in oil. No mutagenic activity was detected using strain TA 100, in the presence or absence of S9 mix with either fried potato slices or potato starch. Mutagenic activity was detected at frying temperatures of 140 degrees C and above. The mutagenic activity was limited to the outer portion of the fried potato slices and increased with frying time and temperature. Mutagenic activity ratios for extraction with both (NH4)2SO4/NH4OH and Na2SO4/NaOH were similar.

Food Contamination↗