[On the problem of the usefulness of the KCN test for staphylococcal diagnosis].
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Cyanides↗
Biomedical subjects
M Witt
Publications and source records attributed to M Witt.
The role of acrosomal enzymes in lymphocytes stimulation by spermatozoa.
We studied the proliferation of human peripheral blood lymphocytes from 20 donors stimulated by acrosomal enzymes from ejaculated spermatozoa from healthy individuals. We extracted the acrosomal enzymes without detergent. The amount of proteins in the acrosomal preparation and its proteolytic activity was estimated. The acrosomal preparation was added to auto- and allogeneic lymphocytes in microculture system. Stimulation of lymphocytes was measured by incorporation of 3H-methyl-thymidine after 3 and 6 days of culture. Auto- and allogeneic lymphocytes that were subjected to the action of an acrosomal preparation in in vitro cultures showed stimulation in a 3-day culture at a final concentration of acrosomal proteins of 3.75-7.5 micrograms per culture. This observation suggests that the mitogenic properties of enzymatic factors are present in acrosomes of spermatozoa.
Acrosome↗
Microsatellite haplotypes of Polish cystic fibrosis alleles: delta F508 chromosomes demonstrate a North-South haplotype frequency gradient.
Analysis of haplotypes of three intragenic, highly polymorphic microsatellite markers (IVS8CA, IVS17BTA, IVS17BCA) of the CFTR gene was performed on a sample of 96 CF chromosomes of Polish origin. Twenty different haplotypes were detected in delta F508 chromosomes. Of these, four haplotypes (23-31-13; 23-32-13; 17-31-13; 17-32-13) represent 67.1% of the overall pool of delta F508 chromosomes. Distribution of these haplotypes, together with frequencies of the delta F508 mutation, are intermediate between Northern- and Southern-European populations and correlate with established gradients.
Cystic Fibrosis↗
[Molecular study of bone marrow chimerism and expression of EVI-1 gene as manifestations of minimal residual disease after bone marrow transplantation (BMT)].
Leukaemias are the most frequent tumour diseases in children. Bone marrow transplantation (BMT) is the most effective therapy for many patients with leukaemia. Highly polymorphic microsatellite markers provide useful genetic markers for detection of complete or mixed chimerism in patients after (BMT). Chimerism can be monitored successfully using several polymerase chain reaction (PCR) techniques and cytogenetic analysis, especially fluorescent in situ hybridization (FISH). It is still unclear whether individuals with mixed chimerism after bone marrow transplantation have an increased risk of developing leukaemic relapse or graft rejection. EVI-1 gene was mapped in human chromosome 3q26. It encodes zinc finger, DNA binding protein detected only in the nucleus. The EVI-1 function is unknown. It is not expressed in normal human haematopoietic cells, but is expressed in leukaemias especially with 3q26 abnormalities. Expression of the EVI-1 may play a significant role in pathogenesis of human leukaemias. Molecular study of chimerism and expression of EVI-1 gene may be useful for monitoring residual disease after bone marrow transplantation.
Bone Marrow↗