[Juvenile diabetes mellitus: decreased prostacyclin (PGI2) synthesis in the vascular wall].
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Biomedical subjects
Publications and source records attributed to M Winter.
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In 1076 sera of healthy volunteer blood donors from Vienna and surroundings HBSAg was positive in 1,7%, HBSAb in 9,5%, HBCAb in 5,7% by radioimmunoassays. HA-Ab was more frequently positive in the rural than in the urban population, its incidence was higher than it was observed by others in Switzerland and in the USA, but lower than in Belgium, Israel, Yugoslavia and Taiwan. 4% of the blood units were positive for HBCAb but negative for HBSAg. The possibility to decrease the incidence of posttransfusion hepatitis by screening for HBCAb is discussed.
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Morphological control of Moncada's bioassay for prostacyclin (PG I2) activity measurement shows that the activity depends not only on endothelium, but in important amounts on subendothelial tissue too. Therefore, it can be concluded that platelet thrombus formation after endothelial cell injury does not depend only on the PG I2-producing ability of the tissue.
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Twenty skin-presensitised Lewis rats received kidney transplants from (Lewis X BN)f1 rats. Two grafts each were withdrawn at intervals from 1--120 min and examined using a scanning electron microscopic (SEM). A series of Lewis to Lewis isografts served as control. In hyperacute rejection at just 1 min spider-like fibrin fibres and platelets could be observed in small arteries, where the endothelium was severely altered. In these regions at 2 and particularly 5 min a fibrin network often contained platelet aggregates, mechanically altered erythrocytes as well as different kinds of leucocytes. This coagulation process progressed with time and resulted in a complete vascular occlusion at 30--60 min.
Recently prostacyclin (PHI2), an unstable prostaglandin with a strong inhibitory effect on platelet aggregation, has been demonstrated in the wall of blood vessels. We estimated the PGI2 availability of arteries and veins in 10 uraemic patients and 12 nephrectomised rats. The production of PGI2 after long-term incubation of the vessels was markedly enhanced and prolonged. This alteration is probably one key mechanism for the deterioration of haemostasis in uraemics. Preliminary results of further studies indicate that substances in uraemic plasma--presumably middle molecules--may enhance the PGI2 availability of normal vessels in vitro.
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Two bone conduction vibrators (Radioear B71 and B72) and a headband (Radioear P-3333) have been developed to meet specifications of both the International Electrotechnical Commission and the American National Standard Institute. Pure-tone thresholds for air conduction and bone conduction were obtained from 24 normal-hearing young adults at audiometric frequencies between 250 and 4000 Hz. Results of this study are in good agreement with the standard air conduction threshold sound pressure levels (ANSI) and with bone conduction threshold force levels reported in the literature.
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