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Biomedical subjects

M Wenzel

Publications and source records attributed to M Wenzel.

At least 109 records · Page 6Linked to original sources
PubMed1987

[Biomicroscopy of an intraocular lens. Comparison of specular microscopy findings with the cytologic specimen].

Seventeen months after implanting an anterior chamber intraocular lens the lens had to be removed because of secondary glaucoma and retinal hemorrhage. The lens had been examined 5 days prior to surgery. It appeared that histiocytic giant cells and fibroblasts had settled on its surface. This was subsequently confirmed by cytological studies. It therefore appears possible to describe an in vivo differential cytology and to diagnose the type of inflammatory reaction in vivo.

Aged↗
PubMed1987

[Cells on intraocular lenses. Comparison of specular and slit-lamp microscopy findings].

Examination with a specular microscope facilitates diagnostic evaluation of cells on the surface of intraocular lenses. Similarly, slit-lamp examination makes specular microscopic studies possible. However, image resolution and magnification are not achieved by using this method, though the amount of time and instrumental effort is minimal. Various types of cells can be differentiated. Fibroblast-like cells can only be demonstrated readily in the specular areas, whereas multinucleate giant cells can usually also be seen by using focal illumination. It is difficult to differentiate the smaller, mononucleated cells.

Fibrin↗
PubMed1987

[Not Available].

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Germany↗
PubMed1987

[Effects of cyclic adenosine-3',5'-monophosphate and cyclo(Lys-Pro).HCl neuronotrophic factors in tissue culture].

Explants and cell cultures of embryonic chick ganglia trigeminalia, telencephalon and retina or hippocampus from fetal rats were incubated in maximow chambers in the presence of cyclic AMP and the dipeptide cyclo(Lys-Pro).HCL under various conditions. Maintenance of nerve cells and growth of nerve fibers were observed by morphometrical methods. 1. Cyclo(Lys-Pro).HCL promoted the maintenance of neuroblasts and the growth of nerve fibres in explants of the ganglion trigeminal and retinal cell cultures. The effect depended on the presence of serum in the medium by use of poly-I-lysine substrate. 2. Extern applicated cyclic AMP and the dipeptide SP3-4 = cyclo(Lys-Pro).HCL facilitated neurite growth in PNS cultures. In the presence of the drugs the length of nerve fibers increased for a short term. On CNS explants substance P (SP1-11) and SP3-4 were without effect. Cyclic AMP stimulated the growth of nerve fibers in CNS explants and cell cultures in number and length. 3. Discussed is the effect of SP1-11 and cyclo(Lys-Pro).HCL for competence of nerve fibre regeneration in vitro in relation to increasing cAMP levels, which may then act as an initial second messenger. It is suggested that explants and cell cultures of nervous tissues will be useful as a tool for the further characterisation of factors with neuronotrophic activities.

Animals↗
PubMed1987

Scanning electron microscopical observations on retina cells in culture.

In cell cultures of the retina from 10 days old chicken embryo photoreceptors, bipolar neurons, synaptic contacts, and supporting cells of Müller were demonstrated by scanning electron microscopy. It was found that the longer the cells survive in culture media they form constantly growing cell aggregates. After 12 days in vitro large multilayered cell complexes covered by an epithelioid cell layer had developed. Merely at the border of such aggregates typical retinal cells could be observed. The occurrence of a dense epithelioid cell layer covering the aggregate of neuronal cells is interpreted as a general adaptational reaction of the cells to the long lasting survival under in vitro conditions.

Animals↗
PubMed1986

[Not Available].

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Anatomy↗
PubMed1986

[Effect and tolerance of daily 2 X 1 g imipenem/cilastatin in general surgery].

The clinical efficacy, tolerability and safety of imipenem/cilastatin were studied in a open, prospective trial with 63 general surgical patients suffering from bacterial infections. According to study criteria, 48 of the patients were evaluable. Clinical cure was achieved in 47 of these 48 patients (97.9%). The causative organisms were eliminated in 39 of the 47 patients cured. Clinical side-reactions were observed in 4.2% of the 63 patients treated. In 8.3% of these laboratory parameters were changed. 77 of the 78 microorganisms isolated before therapy were sensitive to imipenem (MICs 0.02-2.0 mg/l). In one patient a coagulase-negative staphylococcus with an MIC of 16 mg/l was isolated after five days of therapy.

Adult↗
PubMed1986

[Biochemistry of amino acid derivatives of [103Ru] ruthenocene. Comparison with 131I hippuran].

The potential radiopharmaceuticals: ruthenocenoyl alanine, ruthenocenoyl methionine, 1'-methyl-ruthenocenoyl glycine and its esters were labelled with 103Ru starting from the analogous ferrocene compounds. In a series of tests in mice and rats these substances were compared with hippuran and ruppuran (= ruthernocenoyl glycine, a ruthenocene-amino acid analogue of hippuran). The organ distribution of these compounds was measured at various times after injection. Kidney concentrations of 1'-methyl-ruthenocenoyl glycine and its esters were found to be extremely high, followed by a rapid excretion. In contrast with these compounds, ruthenocenoyl methionine indicated a significantly greater affinity for liver than for kidney, but not for pancreas. Ruthenocenoyl alanine exhibits a high affinity for tumor cells. The advantages of 97Ru labelled radiopharmaceuticals compared with 99mTc or 123I/131I labelled compounds are discussed.

Amino Acids↗
PubMed1986

[Amphetamine analog of ruthenocene as a radiodiagnostic agent: brain affinity of ruthenocenoyl-isopropylamine].

N-alkyl derivatives of 1-ruthenocenoyl-2-aminopropane (Rc-amphetamine) were labelled with 103Ru and their organ distributions were measured in mice and rats. All Rc-amphetamines showed a high affinity for brain and lung. A concentration ratio brain/blood up to 17:1 and lung/blood up to 80:1 was measured. The advantages of 97Ru labelled Rc-amphetamine compared to 123I labelled amphetamine for measurement of regional cerebral blood flow are discussed.

Animals↗
PubMed1986

[Effect of derivatives of substance P dipeptide on nerve fiber growth in tissue culture].

Explants and cells from the peripheral (PNS) and central nervous system (CNS) were cultivated in MAXIMOW chambers. The following four derivatives of Substances P (SP) dipeptide were tested for their effects in concentrations between 10(-4) and 10(-8) mol/l: Lys-Pro X 2HBr, Lys(Z)-Pro X HCl, Lys[Z(NO2)]-Pro X HCl and cyclo(Lys-Pro) X HCl. The results suggest that SP and SP derivatives may modulate nerve fibre outgrowth in vitro. In ganglion trigeminale explants Lys(Z)-Pro X HCl stimulated the growth of nerve fibres. The explant covered areas increased significantly. In telencephalon explants and cell cultures the tested derivatives did not essentially promote neurite outgrowth. In retinal cell cultures incubated with cyclo(Lys-Pro) X HCl and Lys[Z(NO2)]-Pro X HCl the neurite outgrowth index was stimulated in a different way. The neurite length was affected and stimulated in the presence of Lys(Z)-Pro X HCl and cyclo(Lys-Pro) X HCl. Both for survival and differentiation PNS neurons depend on specific biofactors. In sensory ganglia explants SP and especially SP derivatives were shown to improve neuronal survival provided that serum or embryonic extract was simultaneously present in the medium. Even if the dipeptides need other biofactors for their full function the may be discussed as neuronotrophic factors because they ensure survival and general growth capability of the responsive neuroblasts.

Animals↗
PubMed1986

[Scanning electron microscopy and light microscopy studies on the retina of chick embryos in cell culture].

Histological and scanning electron microscopy studies (SEM) suggest a classification of neural elements into five main types: receptor cells, bipolar cells, horizontal, amacrine, and ganglion cells in dissociated cell cultures of the retina from 7 to 10 days old chick embryos. By use of SEM the development of the receptor cell inner segments was observed. At the same time many protrusions were noticed at the receptor cell surface. Specific synaptic contacts between the axons of the receptor cells and the dendrites of the bipolar neurons, as well as unspecific contacts between the pericarya of the receptor cells and other dendrites were demonstrated. The bipolar neurons showed smooth cell surfaces, however, the horizontal cells appeared rough on the cell surfaces. Müller cells were always found adjacent to the photoreceptor cells.

Animals↗