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Biomedical subjects

M Wells

Publications and source records attributed to M Wells.

At least 181 records · Page 10Linked to original sources
PubMed1986

Immunohistological characterization of endometrial gland epithelium and extravillous fetal trophoblast in third trimester human placental bed tissues.

The distribution, morphology and antigen expression of endometrial glands, uterine vessels and fetal trophoblast have been studied in third trimester placental bed tissues with a panel of monoclonal antibodies in immunohistochemical techniques. Residual endometrial glands were numerous but often were attenuated or compressed and could only be identified clearly with epithelial cell markers. These glands must be clearly distinguished from vessels and trophoblast in immunological studies of cells in the placental bed. The changing pattern of antigen expression of both maternal glands and fetal trophoblast in placental bed tissues may indicate a form of local regulation of gene expression.

Antibodies, Monoclonal↗
PubMed1986

Cervical glandular atypia associated with squamous intraepithelial neoplasia: a premalignant lesion?

Recent studies have described premalignant changes in the endocervical epithelium, but morphological criteria for the diagnosis of cervical glandular atypia of lesser severity than adenocarcinoma in situ have not been established. Adenocarcinoma in situ is often associated with cervical intraepithelial neoplasia (CIN). The endocervical mucosa in 105 cases of CIN grade III was evaluated and compared with that of 100 controls. Sixteen cases of cervical glandular atypia and one case of adenocarcinoma in situ were identified, and it was possible to discriminate between these and a range of benign glandular lesions. Interestingly, the control series included two patients with cervical glandular atypia, one of whom on review had had a cone biopsy for CIN. The progression of cervical glandular atypia through adenocarcinoma in situ to invasive adenocarcinoma is known, but the natural history of cervical glandular atypia is as yet uncertain.

Adenocarcinoma↗
PubMed1986

Opioid peptides in rat islets of Langerhans. Immunoreactive met- and leu-enkephalins and BAM-22P.

Previous studies have shown that met- and leu-enkephalins are present in extracts of whole pancreas obtained from guinea pigs and human cadavers. The present studies demonstrate that immunoreactive methionine (met)- and leucine (leu)-enkephalins present in rat pancreas are localized in islets of Langerhans. Immunohistochemical staining of fixed, whole pancreas indicated that only islet endocrine cells were heavily stained when any of four different met- and leu-enkephalin-directed antisera or an anti-BAM-22P (bovine adrenal medulla docosapeptide) antiserum was used. The peptides were characterized by a combination of gel-filtration chromatography, high-performance liquid chromatography (HPLC), and specific radioimmunoassay. Free met-enkephalin content in extracts of rat islets was 90-fold enriched over content in extracts of whole pancreas (1.72 +/- 0.35 versus 0.019 +/- 0.007 pmol/mg protein). Treatment with trypsin and carboxy-peptidase-B of high-molecular-weight peptides extracted from pancreas or islets resulted in release of additional met-enkephalin immunoreactivity, which was 39-fold enriched in islets compared with pancreas (5.90 +/- 0.58 and 0.153 +/- 0.032 pmol/mg protein, respectively). Total islet content (per milligram protein) of met-enkephalin-containing peptides was similar to that reported elsewhere for bovine hypothalamus. The immunohistochemical data as well as the enrichment of extractable enkephalins in islets compared with whole pancreas indicate that essentially all the met-enkephalin present in pancreas is localized in islets, while the presence of BAM-22P immunoreactivity in islets is consistent with biosynthesis of enkephalins in islet cells via a preprohormone, such as that described in the bovine adrenal medulla and rat brain.

Animals↗
PubMed1986

Immunohistochemical characterisation of trophoblast antigens and secretory products in ectopic tubal pregnancy.

Fetal trophoblast populations in ectopic fallopian tube pregnancy have been characterised using immunohistochemical techniques. The distribution of trophoblast membrane antigens, low molecular weight epithelial cytokeratins, epithelial membrane antigen, human chorionic gonadotrophin, human placental lactogen, and pregnancy-specific beta 1-glycoprotein in ectopic tubal pregnancy appears similar to that reported in normal early intrauterine pregnancy. These findings emphasise the potential importance of ectopic pregnancy in the study of human gestation.

Antigens, Surface↗
PubMed1985

Ovarian strumal carcinoid tumor with amyloid stroma--report of a case with 20-year follow-up.

We report a case of strumal carcinoid tumor arising in the left ovary of a 20-year-old woman. Histologically, microfollicular, solid, and trabecular areas were present, and amyloid stroma was demonstrated. Many cells contained argyrophilic granules, and thyroglobulin and calcitonin were demonstrated immunohistochemically in several areas. Ultrastructurally the cells resembled thyroid follicular cells and contained neurosecretory granules. It is concluded that strumal carcinoid is of endodermal origin, and shows evidence of thyroid and C-cell differentiation.

APUD Cells↗
PubMed1985

Coexistent immature teratoma of the uterus and endometrial adenocarcinoma complicated by gliomatosis peritonei.

A 37-year-old nulliparous woman presented with severe vaginal bleeding due to a primary immature teratoma of the uterus (Grade II on the Norris scale). The subsequent hysterectomy specimen showed well-differentiated endometrial adenocarcinoma. Mature glial implants were present on both ovaries and throughout the omentum. Postoperative recovery was uneventful and the patient received a course of pelvic radiotherapy. She has remained well with no evidence of recurrence. Primary benign solid teratoma of the uterus is rare. This appears to be the first reported case of primary immature uterine teratoma.

Adenocarcinoma↗
PubMed1985

The expression of major histocompatibility complex antigens by trophoblast in ectopic tubal pregnancy.

The reactivity of the various trophoblast populations found in ectopic fallopian tube pregnancy with established trophoblast-reactive markers and monoclonal antibodies to MHC antigens has been studied. In ectopic tubal pregnancy fetal trophoblast shows an identical reaction pattern with these antibodies to that seen in intrauterine pregnancy, suggesting that ectopic implantation is not related to an inherent immunological abnormality of fetal trophoblast. However, from this and other studies, it appears that extravillous trophoblast displays an unusual class I MHC antigenic structure. This observation may explain the ability of class I MHC--bearing fetal trophoblast--to survive both in the uterus and at an abnormal implantation site.

Antibodies, Monoclonal↗
PubMed1985

Histochemical study of endocervical glycoproteins throughout the normal menstrual cycle and adjacent to cervical intraepithelial neoplasia.

Intracellular endocervical mucins from 45 normal uteri at different phases of the menstrual cycle, assessed by endometrial histology, have been investigated quantitatively. Both acidic and neutral mucins are produced by mucosal cells, although acidic mucin is far in excess of neutral mucin. Both acidic components undergo cyclical changes: an increase in sialoglycoproteins occurs in the periovulatory phase with a decrease in the secretory phase when sulphomucins increase. This suggests that sialoglycoprotein plays a role in the production of sperm-penetrable mucus at midcycle. Endocervical mucins adjacent to 37 examples of cervical intraepithelial neoplasia have also been investigated. In nine cases glands immediately adjacent to the abnormal squamous epithelium showed a decrease in sulphomucins together with an increase in sialomucins when compared with others 8 mm distant. Such changes are similar to those described adjacent to colonic adenocarcinoma and may reflect a premalignant mucosa.

Carcinoma in Situ↗
PubMed1985

Anti-lipopolysaccharide toxin therapy for whole body X-irradiation overdose.

Death in humans from ionising radiation overexposure in the 3-8 Gy (300-800 rad) range is in part due to the toxaemia caused by the entry of gram-negative bacteria and/or their lipopolysaccharide toxin (LPS) into the blood circulation through the walls of partially denuded gut. Anti-LPS hyperimmune equine plasma was evaluated for its ability to lower irradiation-induced lethality. Mice were irradiated with 6.3 Gy (630 rad) and six days later received equine Anti-LPS hyperimmune plasma, control plasma or saline. Mortalities in the three groups were 58%, 92% and 79% (p less than 0.01) respectively. Thus Anti-LPS may prove useful as an adjunct to conventional therapy in treating radiation sickness.

Animals↗
PubMed1985

The microslicing method in the study of temporal bone changes in the perinatal period: an interim report.

The microslicing method was used to study the temporal bones of 49 perinatal deaths in the gross. X ray of the temporal bones was an important part of the investigation. The principle pathological changes were clearly observed. They were: acute otitis media in two cases; an anterior crural deformity of the stapes in four infants and haemorrhage of the apex of the fundus of the internal auditory meatus in 12. Primitive mesenchymal tissue was frequently seen in the infants of younger gestational age, but had largely disappeared by term.

Ear Diseases↗
PubMed1985

Gastric epithelial mucus--a densitometric histochemical study of aspirin-induced damage in the rat.

In an attempt to elucidate which mechanisms of mucus barrier breakdown are important in the formation of gastric erosions, changes occurring in the epithelial mucin content of gastric mucosa exposed to a damaging agent were studied quantitatively. Male Wistar rats (n = 30) were starved for 24 h, then dosed orally with aspirin suspension (300 mg/kg body weight). Animals were killed after 3, 5 or 9 h. Paraffin sections of gastric mucosa were prepared from each and were stained with Alcian blue pH 2.5 (for acidic glycoproteins) or PAS (neutral glycoproteins). Control animals (n = 15) were treated identically, apart from the omission of aspirin from the suspension. The mean integrated optical density (IOD) of each slide was measured using a Vickers M85 Microdensitometer. The mean IOD for PAS-stained sections dropped to 50% of the control value in the 3-h group (P less than 0.001), returning to 84% of the control value in the 5-h group and normal in the 9-h group. In contrast, mean IOD for Alcian blue stained sections was only marginally reduced after 3 h, but showed considerable reduction in the 5-h (57% of control value) and 9-h (61% of control value) groups. It is suggested that this diminished epithelial mucin content is a consequence of cellular exfoliation. Such depletion of epithelial mucin content represents a diminished source of supply for the surface gel, the reduced thickness of which would compromise the 'mucus-bicarbonate' barrier.

Animals↗