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Biomedical subjects

M Waterson

Publications and source records attributed to M Waterson.

6 recordsLinked to original sources
PubMed1987

The production and use of monoclonal antibodies to measure apolipoprotein B by a competitive enzyme-linked immunoassay.

The production of monoclonal antibodies to human lipoproteins is described. One of these antibodies, which was shown to be specific to apolipoprotein B, was used to develop a competitive enzyme-linked immunoassay for apolipoprotein B in serum samples. The antibody selected recognises apolipoprotein B in both low density and very low density lipoprotein particles, but there is no cross-reactivity with high density lipoprotein. There is no requirement for labelling of antigen or antibody used in the assay, and results obtained correlate well (r = 0.88) with measurements on serum samples using a radial immunodiffusion assay for apolipoprotein B.

Animals↗
PubMed1985

Tissue hypoxia during fulminant hepatic failure.

To assess the adequacy of tissue oxygenation in fulminant hepatic failure, we measured arterial oxygen delivery, the affinity of hemoglobin for oxygen, mixed venous oxygen tension, and lactate concentration in 32 patients suffering grade IV encephalopathy. In the patients who died, median systemic vascular resistance and oxygen extraction ratio were significantly (p less than .005) lower than in those who survived (1268 vs. 1866 dyne . sec/cm5 . m2 and 20% vs. 25%, respectively) despite a significantly (p less than .01) greater oxygen delivery in the former group (716 vs. 570 ml/min . m2). Furthermore, nonsurvivors had significantly greater in vivo P50 and mixed venous lactate values (31 vs. 29.5 torr [p less than .02], and 5.1 vs 3.0 mmol/L [p less than .05], respectively). Only in survivors was the in vivo P50 related to the oxygen extraction ratio (r = 0.68 and p less than .01, compared with r = 0.03 in nonsurvivors). These results suggest that the fall in systemic vascular resistance is related to some form of arteriovenous shunting and that this is more severe in patients who die. The subsequent development of tissue hypoxia is an important prognostic factor in fulminant hepatic failure that may contribute to the occurrence of irreversible multiple organ failure.

2,3-Diphosphoglycerate↗