Biomedical subjects

M Washio

Publications and source records attributed to M Washio.

At least 109 records · Page 6Linked to original sources

PubMed1993

Modulation of cardiac myocyte beating rate and hypertrophy by cardiac fibroblasts isolated from neonatal rat ventricle.

We evaluated the in vitro modulation of cardiac myocyte beating rate and growth hypertrophy in the presence or absence of cardiac fibroblasts. Cardiac myocytes and fibroblasts were isolated from neonatal rat ventricles, and cultures of either myocytes alone or of myocytes in co-culture with fibroblasts were established and observed for 21 days. The beating rate in control myocytes increased rapidly, reached peak values on day 5 (266.2 +/- 58.2 beat/min), and then decreased by day 21. The beating rate of myocytes which were co-cultured with fibroblasts in group 2-1 (2-1 ratio of myocytes to fibroblasts, 6.0 x 10(5) total cells/well) was significantly lower than that in control myocytes, and showed a decreased peak value on day 7 (135.1 +/- 46.4). The beating rate in group 1-1 was significantly less than that of group 2-1, with a peak value on day 7 (85.9 +/- 24.9). Group 1-2 myocytes did not show a beating rate increase. Myocyte hypertrophy was measured after three days of culture. The surface area of myocytes which had been co-cultured with fibroblasts was significantly increased, as compared to that of control myocytes. In addition, myocyte surface area increased with increasing numbers of fibroblasts (mean myocyte surface areas in group 2-1, 1-1 and 1-2 were 143 +/- 21, 163 +/- 33, 206 +/- 38% of control, respectively). In conclusion, suppression of the myocyte beating rate and acceleration of myocyte hypertrophy are characteristic expressions of myocyte differentiation and growth during the neonatal period The present results suggest that fibroblasts play an important role in controlling myocyte development.

Animals↗

PubMed1993

Acyclovir-resistant herpes zoster encephalitis successfully treated with vidarabine: a case report.

A 78-year-old man developed herpes zoster virus (HZV) encephalitis. Initially, treatment with aciclovir (750 mg per day) improved CSF cell count and protein level. During the treatment, however, encephalitis in the patient deteriorated in spite of the treatment with aciclovir, suggesting that HZV in the patient had become resistant to aciclovir. Subsequent treatment with vidarabine (600 mg per day, for 15 days) resulted in dramatic improvement in CSF pleocytosis. About two months after the discontinuation of vidarabine, the CSF cell count was normal. The patient became alert gradually, but his amnestic syndrome remained unchanged. Vidarabine may be recommended in the treatment of HZV encephalitis when aciclovir is not effective.

Acyclovir↗

PubMed1993

Effects of anti-hyperlipidaemic agent or dietary protein restriction on progressive renal deterioration in adriamycin-induced nephropathy in rats.

Hyperlipidaemia is strongly suggested to contribute to the pathogenesis of glomerular sclerosis. This study intends to clarify the effects of either a lipid lowering agent, lovastatin (LO) or protein restriction (PR) on adriamycin (ADR)-induced progressive glomerular sclerosis in rats. During the study period both serum cholesterol and triglyceride were significantly reduced in ADR-injected rats with either lovastatin administration (ADR-LO) or protein restriction (ADR-PR) compared to those without such treatment (ADR-NP). At week 22, urinary protein excretion, serum creatinine, blood urea nitrogen, glomerular sclerosis and tubulointestinal alterations, which were marked in ADR-NP, were ameliorated in both ADR-LO and ADR-PR rats. ADR-LO resulted in a body weight similar to that in ADR-NP while ADR-PR induced a marked weight loss. An antihyperlipidaemic agent, such as lovastatin, seems to be a useful tool for the prevention of renal deterioration.

Animals↗

PubMed1993

[The relation between the time and the fatigue of skeletal muscle ventricle].

It is generally recognized that if skeletal muscle is stimulated corresponding with the heart rate to assist the cardiac performance, the contraction power of the skeletal muscle decreases in a few minutes. It becomes necessary that the skeletal muscle is electrically stimulated in 6-9 weeks to lead into the transformation or that the muscle is laid without working after operation in 6-9 weeks to wait for growing of the collateral vessels. The observation time of the fatigue phenomenon in many studies is, however, short and it is not understandable that the contraction power decreases near to zero although the type I fibers remain. So we studied the relationship between the time and the pressure assist of the skeletal muscle ventricle (SMV). It was concluded that 1) the cause of the power decrease is not only the fatigue but the operative invasion. 2) in our setting, when SMV was stimulated in the ratio of 1:4 or 1:10 to the heart rate, the power recovered within 3 hours. 3) if the initial setting of the drive is light enough, the preconditioning or the vascular delay will be unnecessary and the working transformation or the in situ training will practicable.

Animals↗

PubMed1993

[Post operative embolism and left atrial enlargement in the old-aged ASD (atrial septal defect) patients].

As the operation risk of ASD is little today, the unexpected postoperative complication such as embolism is a miserable concern. We experienced two cases of embolism, which were cerebral infarction, in old-aged patients after ASD correction. It has been reported that low cardiac function, atrial fibrillation and foreign body in the left atrium should be risk factors of the embolism after ASD correction. However, blood stasis in the left atrium due to its enlargement may be an essential risk factor of the embolism. Accordingly we evaluated the left atrial volume in 87 ASD patients without complicated cardiac disorder. There was a significant correlation between the age (X) and left atrial volume index (Y). (Y = 16.5 + 1.1X, r = 0.668, p < 0.001). Left atrial volume index in our two cases of cerebral infarction indicated 171.7, 190.8 ml/m2 respectively. The former case had sinus rhythm and its defect was directly closed, and the latter had atrial fibrillation and its defect was closed with teflon patch. In addition pulmonary artery pressure and cardiothoracic ratio correlated with left atrial volume index significantly. Although some investigators reported that pulmonary hypertension and cardiomegaly were the risk factors of embolism, the left atrial enlargement by aging was thought to be the main risk factor. Thus, we concluded that anticoagulant therapy was necessary for the ASD patients with marked left atrial enlargement.

Aged↗

PubMed1993

[In vitro evaluation of prostaglandin I2 analogue on hypothermic injury to immature myocytes].

We evaluated the functional and biochemical effects of prostaglandin I2 analogue (PGI-A) on cardiac myocytes incubated under hypothermic conditions. Myocytes were isolated from neonatal rat ventricles and cultured for 4 days. Then, myocytes (12.5 x 10(5) myocytes/flask) were incubated at 4 degrees C for 24 hrs in media with PGI-A as follows; O M PGI-A (group C: control), 10(-9) M (group P1), 10(-8) M (group P2), 10(-7) M (group P3), 10(-6) M (group P4). After hypothermic incubation, CPK and LDH were measured. The myocytes were then cultured for 24 hrs at 37 degrees C to evaluate the recovery of myocyte beating rate. For the beating rate, group P2 showed significantly increased recovery compared to the control (P2: 47.9, p < 0.025, C:18.1 percent of control; ie, beating rate prior to hypothermic incubation). The release of CPK and LDH was significantly suppressed in group P2 compared to the control (P2:57.7, p < 0.05, 275.1, p < 0.025; C:96.8 mIU/flask, 439.6 mIU/flask, respectively). In conclusion, prostaglandin I2 has direct cytoprotective characteristics for immature myocytes that may be suitable for cardiac preservation.

Animals↗

PubMed1993

[A case of treatment of the Paget-Schroetter syndrome with PTA and Gianturco' expandable metallic stent].

We report a effective management of the Paget-Schroetter syndrome. A 23-year-old man was seen with a complaint of arm swelling and venous engorgement of the left arm. A first-rib resection was performed through a infraclavicular approach with removal of compressive elements including scalenus anterior and subclavius muscles. He became asymptomatic. But 2 months later, recurrent symptoms developed and venograms demonstrated the presence of restenosis of the subclavian vein with extensive collaterals. To relieve symptoms a percutaneous transluminal angioplasty (PTA) and a insertion and placement of the Gianturco's expandable metallic stents was carried out for the remaining stenosis of the subclavicular vein and a residual compression. Venograms, 7 months after operation, showed widely the patent subclavian vein. Trans-infraclavicular approach allowed exposure of the stenotic or obstructed segments of the subclavian vein. The PTA and stenting, especially after removal of compressive elements or during chronic phase, was a very useful and effective procedure for the treatment of the Paget-Schroetter syndrome.

Adult↗

PubMed1993

[A case of hypertrophic obstructive cardiomyopathy using intraoperative trans-esophageal echocardiography].

A 45-year-old man underwent trans-aortic myectomy for hypertrophic obstructive cardiomyopathy (HOCM). Although two mounds of hypertrophic septal muscle were resected, Brockenbrough phenomena proved unsatisfactory myectomy. Intraoperative trans-esophageal echocardiogram (TEE) revealed a residual mound at the apical septum. After the additional myectomy, Brockenbrough phenomena was disappeared. Intraoperative TEE is an useful supplement in the operation of transaortic myectomy for HOCM.

Cardiomyopathy, Hypertrophic↗

PubMed1993

[A case of intrapleural rupture of pulmonary arteriovenous fistula].

The hemothorax due to rupture of arteriovenous fistula is very rare and only 6 cases of this complication have been reported in Japanese literature. 44-year-old male complained of chest pain and dyspnea. A chest roentgenogram revealed right pleural effusion and an abnormal pulmonary shadow. By further examination including pulmonary angiography, the rupture of pulmonary arteriovenous fistula into right pleural cavity was diagnosed. Partial resection of the right lung containing arteriovenous fistula was successfully performed and postoperative course was uneventful.

Adult↗

PubMed1993

[A hanger for thoracic surgery].

A new hanger for thoracic operation is introduced. This hanger for draping cloth is made for the purpose 1) of isolating the operative field from the anesthetist, 2) of keeping the broad hygienic area around the operator, 3) of keeping the area to handle the endotracheal tube for the anesthetist, 4) of keeping the area on the cranial side of the patient where the second assistant is able to stand, 5) of keeping the area for the watcher to stand.

Humans↗

PubMed1993

[A case report of mitral valve aneurysm].

A case of aneurysm of the mitral valve associated with aortic valve regurgitation is presented. The patient was 45-years-old female who complained of palpitation and underwent both aortic and mitral valve replacement. We could not detect the aneurysm by the transthoracic echocardiography because of it's small size (14 mm in diameter). The transesophageal echocardiography showed the aneurysm of the anterior mitral valve leaflet toward the left atrium clearly. This report describes that transesophageal echocardiography appears to be available for accurate diagnosis of the mitral valve disease, especially for the mitral valve aneurysm.

Aortic Valve↗

PubMed1993

[Intracavitary suction for treating giant bulla].

Examination was made of 9 consecutive patients who underwent intracavitary suction for giant bulla. A second operation was required for one patient who had multiple giant bullae. In the other 8 patients, improvement of pulmonary function and symptoms was obtained immediately following the operation. This was particularly more apparent in patients with poor pulmonary function. The following results were obtained. Functional recovery was evident from the early postoperative phase. A one-stage operation was possible. Reduction in postoperative drainage time was realized by bronchial occlusion. The present operation is particularly applicable to compromised patients. Computed tomography scanning is essential for evaluation of the drainage site. For giant bulla with some septation and multivesicular, the present mode of treatment would not be indicated.

Adult↗

PubMed1993

[Suppurative mediastinitis after open heart surgery: in comparison between infants-children and adults].

Among 361 consecutive patients who underwent open surgery from Jan. 1987 to Sept. 1991, risk factors and clinical courses were analyzed retrospectively in comparison between infants-children and adults. Seven mediastinitis (4.0%) occurred in 173 adult patients (20 to 75 y/o, mean: 54.4 y/o) and were not associated with age, sex, type of disease, and duration of operation or cardiopulmonary bypass. Postoperative mediastinitis significantly increased in the patients with low output syndrome (LOS) determined as use of IABP and/or assistant circulations (p < 0.001) and reexploration for bleeding or tamponase was associated with an increased risk for mediastinitis (p < 0.01). Five mediastinitis (2.7%) occurred in 188 infants and children (0 to 17 y/o, mean: 4.2 y/o). All patients involved with mediastinitis were less than 12 month old (2.6 +/- 3.3 month). None of the other factors was associated with an increased risk for this complication. Bacterial cultures of exudate were positive in 11 of 12 patients, and identified as MRSA in 10 and Staphylococcus epidermidis in one. In the seven of adult patients, two developed sepsis and four died with other organic failures or mediastinal bleeding. All five of infants healed after postoperative 33 to 145 days. The immature state of immune response might associate with postoperative mediastinitis in infants, whether LOS may be important in the immune suppression by surgical stress in adults, and the prognosis of mediastinitis might be effected by prolonged depression of postoperative cardiac function in adult patients.

Adolescent↗

PubMed1993

[Minitracheotomy].

Twenty-four consecutive patients undergoing minitracheotomy were reviewed. Postoperative sputum retention was the major indication. In one case the procedure was not possible. Nineteen patients made an uneventful recovery, and decanulation was done in 17. In four patients minitracheotomy treatment was discontinued because formal tracheotomy was performed subsequently. This method is much simpler, less invasive, and more advantageous than formal tracheotomy. It can also be used for the patients after median sternotomy. On the other hand, in the patients with misswallowing because of recurrent nerve palsy etc., conventional tracheotomy should be performed.

Aged↗

PubMed1993

[Experimental procedure for evaluation of myocardial protection using cultured cardiac myocyte--functional and biochemical effects of hypothermic preservation].

The purpose of the present study was to evaluate the functional and biochemical effects on cardiac myocytes cultured under hypothermic conditions. Cardiac myocytes were isolated from neonatal rat ventricles by collagenase dispersion and cultured for 4 days with MCDB 107 medium containing 2% FCS. Thereafter, the myocytes (12.5 x 10(5) myocytes/culture flask) were incubated at 4 degrees C for 6, 12, 18, 24, 36 or 48 hours. After each incubation, CPK and LDH were measured. The myocytes were then cultured for additional 24 hours at 37 degrees C to evaluate the recovery of myocyte beating rate. The recovery ratio of myocyte beating rate was well maintained at 6 and 12 hours (94.5, 103.4 percent of control: ie, beating rate prior to hypothermic incubation, respectively). Thereafter, the recovery ratio significantly decreased at 18 hours (58.4 percent, p < 0.05), and reached null levels at 48 hours. Release of CPK and LDH increased significantly at 18 hours (CPK: 131.8 mIU/flask, p < 0.05; LDH: 356.0 mIU/flask, p < 0.05), and showed a marked increase at 48 hours (CPK: 961.3, LDH: 1729.5). In addition, the CPK and LDH levels did not significantly increased at 37 degrees C for 48 hours compared to those for 6 hours (6 hours: 7.95, 193.7; 48 hours: 13.5, 199.4, respectively). In summery, 4 degrees C hypothermia induced the myocyte injury both functionally and biochemically, which increased with an increasing incubation time. In addition, it was suggested that an optimal temperature in hypothermic preservation might be 10 degrees C. Thus, this cell-culture system may provide a useful and simple method for in vitro evaluation of hypothermic preservation.

Animals↗

PubMed1993

[Palliative management of left atrioventricular valve atresia with normal aortic valve].

We present three cases of the left atrioventricular valve atresia (LAVVA) with normal aortic valve. All cases showed [S,D,D] and had concordant AV connection. First case had two functional ventricles with multiple VSD and ventriculo-arterial connection was normal. Interatrial communication was maintained through ASD and the infant underwent pulmonary arterial banding (PAB). Four years later, the patient underwent Blalock-Hanlon (B-H) procedure for an inadequate ASD. Second case showed the univentricular heart of right ventricle with double outlet. In this case, PFO was restrictive and the infant underwent B-H procedure. Ten days after initial palliation, patient underwent PAB. Third case showed the univentricular heart of left ventricle and transposed great arteries. This patient had also the coarctation of the aorta. B-H procedure, PAB and subclavian flap aortoplasty were performed simultaneously by bilateral thoracotomy. All patients have done well, with normal growth and development at the time of their last outpatient evaluation at 10 year, 9 months and 9 months after surgery respectively.

Aortic Valve↗

PubMed1993

[A depression of respiratory function following the cardiopulmonary bypass in infants and children].

Fourty-one patients (2-44 month old) underwent the closure of ventricular septal defect using moderate-hypothermic cardiopulmonary bypass (CPB). The respiratory index (RI) were monitored after CPB, as functional parameters of oxygenation capacity. At 15 min after CPB, there was the increased level in RI (2.22 +/- 0.45; normal value: 0.1-0.37). Thereafter, RI gradually decreased by 90 min after CPB (1.22 +/- 0.19), and again increased reaching peak levels by 12 hrs after ICU-entry (2.56 +/- 0.64). The patients who exceed 1.00 at 15 min after CPB (RI-15) remained higher levels in RI compared to less than 1.00 and had the prolonged controlled ventilation. The RI-15 correlated with pulmonary-systemic flow ratio (Qp/Qs) and pulmonary-left ventricular pressure ratio (PA/LV). There was no significant correlation between RI-15 and CPB factors including CPB time, dilution rate, rectal temperature, flow and perfusion pressure. These results suggest that pulmonary dysfunction following the CPB may not be simply occurred by CPB but also involved by preoperative conditions. An increased pulmonary blood flow might be one of important and predictive factor for the depression of oxygenation following the cardiac surgery in infants and children.

Cardiopulmonary Bypass↗

PubMed1993

[Hygienic handling in cardiac surgery].

Some points regarding the hygienic handling in cardiac surgery are mentioned. The sternal infection or mediastinitis is still one of the most important complications after cardiac operation especially when ITA is used for CABG. After we paid much attention to these points, the postoperative sternal infection has decreased obviously.

Cardiac Surgical Procedures↗