Biomedical subjects

M Waseem

Publications and source records attributed to M Waseem.

40 records · Page 3Linked to original sources

PubMedDate not supplied

Cytotoxic and hemolytic action of coal-quartz mixtures.

The effect of coal-quartz mixtures that contained graded amounts of coal or quartz was studied using peritoneal macrophage cultures and plasma-free washed sheep erythrocyte suspensions. A stoichiometric relationship was observed between the quartz content of coal-quartz mixture and the amount of lactic dehydrogenase liberated extracellularly and acid dye uptake by macrophage monolayers. Total lactic dehydrogenase of the monolayers plus supernatant remained unaltered following exposure to different dust mixtures. Dusts containing a higher concentration of quartz also produced a greater degree of hemolysis. Coal did not inhibit the quartz-induced macrophage cytotoxicity or erythrocyte hemolysis.

Animals↗

PubMedDate not supplied

Pulmonary effects of butylated hydroxytoluene in mice.

By virtue of its antioxidant property butylated hydroxytoluene (BHT) is extensively used to prevent rancidity of food materials on storage. Recently pulmonary effects of BHT ingestion have been reported. Present studies were aimed at exploring the effect of BHT on the lungs of mice. Cellular, biochemical and histopathological changes were studied. Male mice (25-30 g) were given BHT intraperitoneally in doses of 200, 400 or 800 mg/kg bw in olive oil. The controls received olive oil alone. The animals were sacrificed at 24 h, 48 h and 7 day intervals and the bronchoalveolar lavage fluid (BALF) was collected for cellular and biochemical studies. Lungs from separate groups of animals were fixed in formal-saline for histopathological studies. The total number of cells in BHT-exposed mice increased in a dose-dependent manner. Total protein content and the activity of LDH also increased significantly as compared with controls. The histopathological results correlated with the biochemical and cellular alterations. It can, therefore, be concluded that BALF analysis can be used to quantify the acute pulmonary toxicity following extrapulmonary exposure to BHT.

Animals↗

PubMedDate not supplied

Successful outcome with methimazole and lithium combination therapy for propylthiouracil-induced hepatotoxicity.

OBJECTIVE: To assess the efficacy and safety of combination therapy with methimazole and lithium carbonate in management of severe thyrotoxicosis and propylthiouracil-induced hepatotoxicity. METHODS: We present a case report of a patient with severe thyrotoxicosis and worsening liver dysfunction after propylthiouracil therapy, and we review the pertinent literature. RESULTS: In a 49-year-old man with severe thyrotoxicosis and propylthiouracil-induced hepatotoxicity, indices of liver function continued to increase despite discontinuation of propylthiouracil treatment. Adjunctive therapy with methimazole and lithium resulted in prompt remission of clinical manifestations and normalization of thyroid hormone indices, as well as a gradual reversal of liver dysfunction. CONCLUSION: Adjunctive therapy with methimazole and lithium is synergistic in promptly achieving a euthyroid state. Therefore, this combination therapy provides a safe and effective alternative option in patients with thyrotoxicosis associated with propylthiouracil-induced hepatotoxicity.

Journal Article↗

PubMedDate not supplied

Reaction of pulmonary macrophages exposed to nickel and cadmium in vitro.

We examined the morphological and functional characteristics of goat alveolar macrophages exposed in a serum-free medium in vitro for up to 20 hr to cadmium as CdCl2 and nickel as NiCl2. The concentration of these metals varied from 0.04 to 1.00 mM. Cadmium reduced the viability and phagocytosis of macrophages and increased the release of lactate dehydrogenase from cells into the culture medium at all the concentrations tested. Alterations in macrophage surface morphology, evaluated by scanning electron microscopy, consisted of withdrawal of pseudopodia and rounding of cells exposed to 0.20 and 1.00 mM of CdCl2. In nickel-exposed macrophages these changes were marked only at higher concentrations of the metal. Higher concentrations of Ni were necessary to produce effects similar to those produced by lower Cd concentrations. Our results demonstrated that the higher toxicity of cadmium as compared to that of nickel for alveolar macrophages is related to a difference in the alterations in the morphology and function of the exposed cells.

Animals↗