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Biomedical subjects

M Warwas

Publications and source records attributed to M Warwas.

48 records · Page 3Linked to original sources
PubMedDate not supplied

Cysteine proteinase inhibitors in human placenta.

When human placental extract was chromatographed on a Sephadex G-75 column, cysteine proteinase inhibitors with molecular weights of 80 000 and 12 300 were eluted. The high molecular weight peak (CPI-H) was identified as alpha-cysteine proteinase inhibitor. The thermostable low molecular weight peak (CPI-L) inhibited plant proteinases (papain, ficin and bromelain) as well as cathepsins B, H and L isolated from the human placenta. No cross-reactivity was observed between placental CPI-L and serum alpha-CPI.

Antibodies↗
PubMedDate not supplied

Anti-acrosin antibodies in sera, colostrum and genital organs of vaccinated ewes: estimation and correlation with fertility.

Three ewes were vaccinated with boar sperm acrosin. Despite the presence of antiacrosin antibodies which crossreact with ram acrosin in the sera of the immunized sheep, no significant reduction in fertility was observed. Incubation of ejaculated or capacitated boar spermatozoa with anti-acrosin serum or IgG did not reduce the halos of digestion in the gelatinolysis test. However, an unexplained decrease of the forward progression and number of lytically active spermatozoa was observed after this treatment. We found antiacrosin antibodies and determined their titers in the sera of the vaccinated ewes, in colostrum and in aqueous extracts of cervix, uterus, oviduct and ovary. No antiacrosin antibodies could be detected in the sera of new born lambs. The immunoglobulins IgA, IgG and IgM were isolated from the sera of the ewes. The acrosin-specific antibodies belonged exclusively to the IgG class. In genital extracts the amount of IgG, IgA and IgM was determined. IgG was found in higher amount in every extract. IgA is present in similar amount only in the cervix, in the other parts only in traces. In all extracts IgM is found in a very low level. The specificity of the antibodies for acrosin was proven by active enzyme staining of the precipitin lines obtained by immunodiffusion.

Acrosin↗
PubMedDate not supplied

[Glycosylated proteins and various acute phase factors in patients with type II diabetes].

In the plasma of patients with type II diabetes a statistically significant increase was found of the concentration of glycosylated proteins, haptoglobin and sialic acid, in relation to controls. The concentration of glycosylated proteins was determined by the fructosamine method and by a method based on reaction with thiobarbituric acid. The "r" correlation coefficient between both methods in the diabetics was 0.58. No significant correlation was found between fructosamine concentration and the determined acute phase factors. The statistical analysis of the obtained results related to the coexistence of macroangiopathy or microangiopathy, diabetes duration or treatment method showed only a significantly higher fructosamine concentration expressed as mM of fructose in relation to one gram of albumin when the group of diabetics with vascular complications was compared with that without these complications.

Aged↗
PubMedDate not supplied

[Diagnostic value of determining alpha1 protease inhibitor and alpha2 macroglobulin in the serum of women with malignant and non-malignant ovarian tumors and uterine myomas].

In the serum of 21 women with malignant ovarian tumours, 13 with uterine myomas and 16 with cysts the concentrations were determined of alpha 1 inhibitor of proteases and alpha 2 macroglobulin by immunodiffusion, and the functional activity of both inhibitors which made possible calculation of the specific activity (units of inhibitory activity per one gram of inhibitor protein). In women with malignant tumours the specific activity of alpha 1 inhibitor was decreased, suggesting that a part of the inhibitor was functionally inactive. This decrease was statistically significant in relation to the control group and the groups of myomas and cysts, but was independent of the degree of disease progression. The specific activity of alpha 2 macroglobulin was significantly decreased only in patients with grade IV degrees of clinical progression (according to FIGO) in relation to the control group and to the group with cysts.

Adolescent↗