Biomedical subjects

M Wang

Publications and source records attributed to M Wang.

At least 343 records · Page 19Linked to original sources

PubMed2000

A cancer gene therapy approach utilizing an anti-erbB-2 single-chain antibody-encoding adenovirus (AD21): a phase I trial.

The purpose of this Phase I study was to determine the feasibility of using an anti-erbB-2-encoding adenovirus (Ad21) to treat erbB-2-overexpressing ovarian cancer. Recurrent ovarian cancer patients were treated i.p. with Ad21 in dosages ranging from 1 x 10(9) to 1 x 10(11) pfu. Patients were monitored after treatment for evidence of clinical toxicity and efficacy. Peritoneal aspirates and serum samples were obtained to assess for evidence of gene transfer/expression, for generation of wild-type vector, and antiadenoviral humoral response. Fifteen patients were treated per study specifications. Treatment-specific grade 1/2 fever was experienced by 9 of 15 (60%) patients. Other transient grade 1/2 constitutional, pain, and gastrointestinal symptoms were also experienced. No dose-limiting vector-related toxicity was experienced. Of 13 patients evaluable for response, 5 (38%) had stable disease and 8 (61%) had evidence of progressive disease. One patient with nonmeasurable disease normalized her CA125 at the 8-week evaluation, and one patient with nonmeasurable disease remained without clinical evidence of disease for 6 months after treatment. PCR analysis of peritoneal aspirates demonstrated the presence of Ad21 in 84.6%, 84.6%, and 61.6% of evaluable specimens at days 2, 14, and 56 after treatment, respectively. No wild-type virus was detected. Reverse transcription-PCR analysis demonstrated expression of the anti-erbB-2 sFv-encoding gene in 10 of 14 evaluable patients at day 2. Five of six evaluable patients had an increase in antiadenovirus antibody titer. This study suggests that adenoviral-mediated gene therapy using an anti-erbB-2-directed intrabody is feasible in the context of human ovarian cancer.

Adenoviruses, Human↗

PubMed2000

Studies of MAL gene in human esophageal cancer by RNA in situ hybridization.

OBJECTIVE: To observe the expression of MAL gene mRNA in esophageal cancer tissue and adjacent epithelial mucosa. METHODS: Non-radioactive RNA in situ hybridization. RESULTS: MAL gene was expressed in all epithelia of 33 matched samples but only in 8 of esophageal cancer tissues. Differential expression between esophageal cancer tissues and adjacent epithelia was not related to clinical parameters. CONCLUSION: Down-regulation of MAL may be an important event in the development of esophageal cancer.

Down-Regulation↗

PubMed2000

A clinical investigation on zhi ling tang for treatment of senile dementia.

Zhi Ling Tang (ZLT [symbol: see text], a TCM prescription designed for replenishing essence, supplementing marrow, invigorating qi, warming yang, removing blood stasis and phlegm, tonifying the brain, and invigorating mental activity) was used in 32 cases of senile dementia (SD). After treatment, the levels of serum cholesterol (Tch), triglyceride (TG), and plasma lipid peroxides (LPO) were lowered; the content of high density lipoprotein (HDL) and the activity of superoxide dismutase (SOD) in RBC significantly elevated, the cerebral blood flow was increased; latent period of P300 and P3 waves was shortened while the amplitude of P3 elevated; and topographic electroencephalogram, revised Hasegawa dementia scales (HDS) scores (P < 0.05-0.01) and clinical symptoms were improved. The total effective rate was 81.3%, indicating that ZLT exhibits relatively good therapeutic effects for treating senile dementia.

Aged↗

PubMed2000

Genetically modified CD34+ cells exert a cytotoxic bystander effect on human endothelial and cancer cells.

We and others have proposed mammalian cells as gene delivery vehicles with the potential for overcoming physiological barriers to viral vectors. To that end, we previously have shown the potential of CD34+ endothelial progenitors for systemic gene delivery in a primate angiogenesis model. Here we seek to explore the utility of CD34+ cells of human origin as vehicles for toxin genes and, in particular, to measure their capacity to effect a cytotoxic bystander effect in human endothelium and tumor cells. To this end, CD34+ cells were transduced with TOZ.1, a nonreplicative herpes simplex vector encoding thymidine kinase. To test the capacity of CD34+ cells to induce a cytotoxic bystander effect in target cells, we performed mixing experiments, whereby TOZ.1-transduced CD34+ cells were mixed with either human vascular endothelial cells or human ovarian tumor cells (SKOV3.ip1). Cell viability was measured by the MTS assay. Lastly, mixtures of TOZ.1-transduced CD34+ cells and SKOV3.ip1 tumor cells were injected s.c. to evaluate the bystander effect in vivo. After transduction of CD34+ cells with TOZ.1, treatment with ganciclovir induced the killing of 99% of cells. In cell-mixing experiments, a linear correlation was observed between the percentages of TOZ.1-transduced CD34+ cells and total cell killing. For example, when 50% of CD34+ transduced cells were mixed with nontransduced SKOV3.ip1, >70% of all cells died. Similarly, when the same percentage was mixed with human vascular endothelial cells, >80% of the total number of cells died. In vivo studies showed an abrogation of tumor formation when TOZ.1-transduced CD34+ cells and ganciclovir were administered. Our observations establish the feasibility of a method for cell-based toxin gene delivery into disseminated areas of tumor angiogenesis.

Animals↗

PubMed2000

14-3-3 proteins and a 13-lipoxygenase form associations in a phosphorylation-dependent manner.

Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. 474, 48-52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms interactions with 14-3-3 proteins. In the present report we show that the presence of 13-LOX and 14-3-3 proteins was established in high-molecular-mass complexes. Amounts of 13-LOX and 14-3-3 proteins in high-molecular-mass fractions increased during germination, but were reduced after dephosphorylation of protein extracts or competition with the 14-3-3-binding peptide P-Raf-259, indicating that 13-LOX and 14-3-3 proteins interact in a phosphorylation-dependent manner.

14-3-3 Proteins↗

PubMed2000

[Study of an in-vivo and real-time method to measure blood viscosity].

The traditional methods to measure blood viscosity are out-line. They have the disadvantages of long measurement time, many disturbance factors during measurement, the damage to blood sample, and etc. So rapidity, accuracy and repeatability are not satisfactory. A new method to measure blood viscosity is proposed in this paper, which uses needle instead of capillary and can measure blood viscosity during collecting blood. Owing to the advantages of small amount of blood sample, rapidity and repeatability, the new method is of value for clinical application.

Animals↗

PubMed2000

[A strain of influenza A H9N2 virus repeatedly isolated from human population in China].

OBJECTIVE: To understand the subtype of surface proteins (hemagglutinin and neuraminidase) and antigenic character of the isolate of influenza virus, as well as to know its origin. METHODS: The virus isolation was performed on MDCK cells. The identification and antigenic analysis of the isolate were carried out with HI and NI tests. The serum antibody in population against the isolate was detected with HI and neutralization assays. The individual investigation of the patient from whom the H9N2 virus was isolated was followed. RESULTS: The isolate was H9N2 subtype of influenza A virus. It was G9 like strain. Its antigenicity was different from that of H9N2 viruses which were previously found in humans, chickens and pigeons. The HI and neutralization titers to H9N2 virus in convalescent serum of the patient reached 1:400 and > or =1: 640, respectively. The HI antibody titer 1:25 to H9N2 virus was also detected in the serum of patient's mother. CONCLUSIONS: The isolate is H9N2 subtype of influenza A virus and belongs to G9 like strain. The antigenic character of the isolate was different from that of H9N2 viruses found previously in humans, chickens and pigeons. The greatest possibility was that her mother had contacted with birds, especially chickens carrying H9N2 virus, then transmitted to her or she breathed in the air borne with H9N2 virus particles directly.

Adult↗

PubMed2000

[Studies on the basis of molecular biology of the phase change of influenza A(H1N1) viruses].

OBJECTIVE: To reveal the basis of molecular biology of the phase change of influenza A (H1N1) viruses. METHODS: Virion RNA was transcribed into cDNA by reverse transcriptase, cDNA amplified by PCR, the products of PCR were purified. Afterward, RNA sequence analysis was performed by the dideoxynucleotide chain termination method, using synthetic oligodeoxynucleotide primers. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign (Version 1.03) and Editseq (Version 3.69) software. RESULTS: No special amino acid on HA1 protein molecule determining the influenza A(H1N1) virus with O or D phase feature was found. However, there are differences of amino acid sequences at -2, -7, 130 and 139 positions between H1N1 viruses isolated in or before end after 1995. It is very interesting that since 1995, two different genetic lineages of influenza A (H1N1) virus HA gene have been cocirculating in men in China. One lineage is no any deletion of amino acid on HA1 protein molecule. The other one has a deletion at 130 position. Their amino acid sequences were compared with those of H1N1 viruses isolated before 1995. CONCLUSIONS: There is no special amino acid on HA1 domain protein molecule determining the influenza A (H1N1) virus with O or D phase feature was found. Since 1995, two distinguished genetic lineages of influenza A H1N1 virus HA gene have been cocirculating in men in China.

Amino Acid Sequence↗

PubMed2000

[Study on the origin of influenza A(H1N2) virus HA and NA genes].

OBJECTIVE: To determine the origins of the HA and NA genes of new subtype (H1N2) of influenza A virus. METHODS: Virus was amplified in embryonated chicken eggs, then virion RNA was transcribed into cDNA by reverse transcriptase, cDNA amplified by PCR, the products of PCR were purified. Afterward, RNA sequence analysis was performed by the dideoxynucleotide chain termination method, using synthetic oligodideoxynucleotide primers. Finally, phylogenetic analysis of the sequencing data was per-formed with MegAlign (Version 1.03) and Editseq (Version 3.69) software. RESULTS: The homology of amino acid sequences of protein molecules on HAl domains when compared, with A/PR/8/34(H1N1) and A/Guangdong/6/91(H1 N1) viruses was 98.2% and 99.4%, respectively. The homology of amino acid sequences of NA protein molecules between the novel reassortant and H1N1 virus was only 83.4 %, but the homology of those between H1N2 strain and H3N2 virus circulating in men in 1995 was as high as 99.1 %. CONCLUSIONS: The new reassortant (H1N2) virus HA gene was derived from A/PR/8/34(H1N1) like virus, but its NA gene was derived from H3N2 virus circulating in men in 1995.

Adult↗

PubMed2000

[Further study on the genetic characteristics of a strain of A/PR/8/34/].

OBJECTIVE: Through the test and analysis of nucleotide sequences of seven different RNA segments of the virion to exclude the possibility that A/Guangdong/6/91 (H1N1) strain might be derived from laboratory contamination and to determine how many RNA segments are there with nucleotide sequence difference between A/Guangdong/6/91 (H1N1) and A/PR/8/34 (H1N1) viruses, as well as to determine is A/Guangdong/6/91 virus a reassortant? METHODS: Virion RNA was transcribed into cDNA by reverse transcriptase, cDNA was amplified by PCR, the products of PCR were purified. Afterward, RNA sequence test was performed by the dideoxynucleotide chain termination method using synthetic oligodeoxynucleotide primers. RESULTS: There are no nucleotide sequence differences in RNA segments 6 and 7 between A/Guangdong/6/91 (H1N1) and PR8 strains, while there are 20,5, 11, 7 and 6 differences in nucleotide sequences of RNA segments 1, 2, 3, 5 and 8 between these two strains, respectively. These differences induce 10, 2, 1, 0 and 4 differences in amino acid sequences on PB2, PB1, PA,NP and NS protein molecules between A/Guangdong/6/91 (H1N1) and PR8 strains, respectively. CONCLUSIONS: The possibility of laboratory contamination of A/Guangdong/6/91 (H1N1) virus can be ruled out completely. A/Guangdong/6/91 (H1N1) virus is a PR8 like strain, but not a reassortant.

China↗

PubMed2000

[The study on hepatitis G virus infection among patients with liver diseases].

OBJECTIVE: To investigate the hepatitis G virus (HGV) infection among patients with liver diseases. METHODS: Anti-HGV was detected by ELISA among 154 patients with liver diseases (no history of blood transfusion), 54 of them, HGV RNA was detected by RT-PCR concomitantly. The rates of anti-HGV and HGV RNA were calculated, respectively. The positivity of HGV RNA was taken as diagnostic criteria for HGV infection in different kinds of liver disease. RESULTS: Of 154 patients with liver diseases, anti-HGV was positive in 31 patients, the detectable rate of anti- HGV was 20.13%. HGV RNA was detected in 13 of 54 patients, the detectable rate was 24.07%. The positive rates of HGV RNA tested in 35 patients with chronic hepatitis and 6 patients with fatty-liver were 20.00% (7/35) and a ratio of 5/6, respectively. CONCLUSIONS: (1) HGV infection is considerably prevalent among patients with non-transfusion transmitted sporadic hepatitis. (2) HGV is a responsible agent for chronic hepatitis. (3) The clinical significance of detectable HGV RNA in patients with fatty-liver remains for further study.

Adolescent↗

PubMed2000

[Parasitic metamorphosis development of Lamprotula fibrosa].

The glochidia of Lamprotula fibrosa develop to maturity in the outer gill of female and are expelled to the outside in winter, and then, the mature glochidia are parasitized to the gill of fish host and start the parasitic metamorphosis development. The parasitic period lasts about 4 months. The inner and outer byssuses disappear after parasitizing for 3 days. The foots develop after 35 days. The intestine, adductor muscle, nephridium and gill anlage develop after 90 days. The shells become thick and protrusive. The glochidia become larvae with a size of 253.37 x 273.26 x 179.96 microns in the next spring, then leave the gill of fish host, and start their independent life.

Animals↗

PubMed2000

[Effect of elevated CO2 concentration on growth of dominant tree species in pine broadleaf forest of Changbai mountain].

Potted saplings of Pinus koraiensis, Picea koraiensis, Larix olgensis, Populus ussuriensis, Betula platyphylla, Tilia amurensis, Traxinus mandshurica, and Acer mono, the eight dominant species in the Korean pine broadleaf forest at Changbai Mountain, were grown in open-top chambers with ambient(400 microliters.L-1) and elevated (700 microliters.L-1) CO2 concentration under natural sunlight for two growing seasons. The average height growth of these eight species under elevated CO2 increased by 10%-40%, compared with the control at ambient CO2. The response of transpiratory rate to the higher CO2 concentration was different among the species, and the water use efficiency of the saplings of the 8 species growing under elevated CO2 concentration was higher than that under ambient CO2. Soluble sugar and chlorophyll contents were not related to the CO2 concentration under which the saplings grew, indicating that the complicacy was existed in the acclimation of tree species to high CO2 concentration. The response of broad-leaved tree species to the elevated CO2 concentration was more sensitive than that of conifer tree species. Saplings of all tree species showed a photosynthetic acclimation to higher CO2 under long-term elevated CO2 concentration.

Carbohydrate Metabolism↗

PubMed2000

[NO emission from winter wheat fields of rice-wheat rotation ecosystem in southeast China].

Measurements of NO emission from the winter wheat field of rice-wheat rotation ecosystem in southeast China during the whole period of wheat growth show that a higher NO emission was observed in spring than in autumn, and almost no emission in winter could be detected. Temperature was the most important factor determining the seasonal variation pattern of NO emission. Although the N fertilization might enlarge NO emission by a factor of 5-7, it could not modify the seasonal variation pattern. During the period of relatively weak plant activity, the diurnal variation pattern, with the maximum emission at 9:00-14:00, was determined by temperature. When plants luxuriated, however, the competition of NH4+ by plant uptake and by microbial nitrification led to a night-peak variation pattern, in which, the maximum diurnal emission usually occurred during 18:00 and 4:00 of next day, and the minimum at 8:00-16:00.

China↗

PubMed2000

[Serum leptin levels during pregnancy].

OBJECTIVE: To study the serum leptin levels in Chinese pregnant women, and to discuss the relationship between leptin level and pregnancy. METHODS: Serum leptin concentrations of 251 pregnant women (13.6 to 30.9 gestational weeks) were measured with radioimmunoassay, and 35 age and body mass index (BMI) matched non-pregnant women were selected as control. Serum leptin concentrations were compared between pregnant and control women, correlations between leptin concentrations and gestational age, weight, BMI, blood pressure and other parameters were analyzed. RESULTS: In these pregnant women, aged (28.5 +/- 3.9) years, BMI (23.6 +/- 2.8) kg/m2, serum leptin concentrations were (15.1 +/- 7.4) micrograms/L; while in control women, aged (27.4 +/- 3.8) years, BMI (22.6 +/- 2.5) kg/m2, serum leptin concentrations were (9.1 +/- 4.9) micrograms/L. The mean age and BMI are comparable between 2 groups. Serum leptin concentrations in pregnant women were much higher than those in control women (P < 0.001). Within the pregnant women, serum leptin concentrations were significantly correlated to body weight(r = 0.529), BMI (r = 0.551,), waist circumference (r = 0.564), height of uterus bottom (r = 0.151), systolic blood pressure (r = 0.134) and diastolic blood pressure (r = 0.251). During pregnancy, serum leptin concentrations rose along with the gestational age, although no statistical difference was revealed up to 30th gestational week. CONCLUSIONS: Leptin is a hormone intimately related to the pregnancy, its concentrations are much higher during pregnancy than those of non-pregnant women with matched age and BMI. Like in non-pregnant women, leptin levels are significantly correlated to body weight and BMI, and also correlated to blood pressure, the correlation between leptin and diastolic blood pressure is more evident, indicating that serum leptin may serve as a marker for detecting and monitoring pregnancy complications.

Adult↗

PubMed2000

[Molecular cytogenetic study of bladder transitional cell carcinoma by FISH].

OBJECTIVE: To study the molecular cytogenetic alterations of transitional cell carcinoma of the urinary bladder with exfoliated cells. METHODS: Fluorescence in situ hybridization (FISH) was performed using centromeric probes of chromosome 7, 9, 11 and 17 to examine chromosome aberration of exfoliated cells in 34 urine samples and 30 bladder washings from patients with transitional cell urinary bladder carcinoma. RESULTS: (1) The frequency of numerical aberration of chromosome 7, 9, 11, 17 was 23.5%, 38.2%, 14.7% and 11.8% in urine and 30.0%, 50.0%, 26.7% and 16.7% in bladder washing, respectively. Loss of chromosome 9 was the most common finding, but it was not correlated with pathological grade of cancer and stage of the disease. Abnormality of chromosome 7 was however associated with the clinical stage. (2) The positive rate of examination by cytology and FISH was 29.4% and 55.9% in urine, 27.6% and 73.7% in bladder washing, respectively. When the results of cytology and FISH were combined, the positive rate increased to 67.6% and 80.0% for urine and bladder washing, respectively. CONCLUSION: A number of chromosome aberrations is detected in transitional cell carcinoma of the urinary bladder by FISH technique which provides a basis for further understanding of its molecular pathogenesis and clinical applications.

Adult↗

PubMed2000

[Combined five tumor markers in detecting primary hepatic carcinoma].

OBJECTIVE: To increase the detection rate of primary hepatic carcinoma (PHC) and to diagnose PHC earlier. METHODS: AFP was combined with r-glutamyle transpeptidase (r-GT), alpha-fucosidase (AFU), tumor necrosis factor-alpha (TNF-alpha) and DR-70. RESULTS: The positive detection rate of PHC negative AFP with combined four markers was 9.4%. The total positive detection rate of PHC with combined five tumor markers reached 98.0% which was significantly higher than that with AFP (P < 0.01). CONCLUSIONS: The positive detection rate of PHC can be increased by combined five tumor markers. It is helpful in diagnosing PHC earlier and can differentiate PHC from liver cirrhosis.

Biomarkers, Tumor↗

PubMed2000

[Fresh femoral shaft fractures treated with un-reamed intramedullary nail: 203 cases].

OBJECTIVE: To discuss the experience treating fresh femoral shaft fractures with un-reamed intramedullary nail. METHODS: 203 cases of fresh femoral shaft fractures who had been treated with un-reamed intramedullary nail from November. 1995 to January. 1999 were analyzed retrospectively. 18 cases were open, classified as Gustilo I, and. 185 cases were closed. Traction table and image intensifier were used during operation. Closed reduction, un-reamed and free hand distal locking technique were used. Active movements of nearby joints were encouraged and partial weight bearing of 10 - 15 kg were allowed right after operation. RESULTS: All 203 cases of fresh femoral shaft fractures got bone union within 4 - 6 months. No case of delayed union and infection happened. 1 distal locking screw was broken. All patients gained normal extremity functions. CONCLUSION: Un-reamed intramedullary nailing of fresh femoral shaft fracture has less damage to the local soft tissue and blood supply. It has a relative stable fracture fixation, and less interruption to the healing procedure. It is one of the best methods for treating femoral fracture.

Bone Nails↗