Biomedical subjects

M Wang

Publications and source records attributed to M Wang.

At least 235 records · Page 13Linked to original sources

PubMed2001

[A study on the resources coping with occupational stress in teachers].

OBJECTIVE: To explore the status of the resources coping with occupational stress in teachers. METHODS: Occupational stress inventory revised edition (OSI-R) was used to measure their occupational stress, strain and psychological coping resources for 1,460 teachers in primary and secondary schools and 319 non-teacher intellectuals. Analyses were focused on coping resources of teachers. RESULTS: The higher the level of coping resource of teachers, the lower the personal strain in them, with an inverse correlationship. Coping resource in the teacher group was significantly higher than that in the non-teacher group, with scores of (130.4 +/- 18.2) and (126.9 +/- 19.1), respectively. Coping resource in teachers decreased with the increase of age, with the scores from (134.1 +/- 18.1) in the group aged less than 30 to (128.5 +/- 17.5) in the group aged 50. Coping resource in the female teachers was significantly higher than that in the male teachers, with scores of (131.4 +/- 18.3) and (129.4 +/- 18.1), respectively. Coping resource in the primary school teachers was significantly higher than that in the secondary school teachers, with scores of (132.7 +/- 18.1) and (128.5 +/- 18.1), respectively. CONCLUSION: It is important to enhance teacher's coping resource, especially for the male teachers in the secondary schools, for improving their teaching efficiency.

Adaptation, Psychological↗

PubMed2001

[Compound RSA 11 beta-hydroxylation with Curvularia lunata].

Compound RSA 11 beta-Hydroxylation of Curvularia lunata was studied. The composition of hydroxylation products was analyzed. It was found that the conversion rate with protoplasts was increased conspicuously, the construction and quantity of hydroxylation products was not alteration, but the percentage was changed greatly. The 14 alpha-OH percentage of protoplasts was 3.03-fold than that of mycelia, the 11 alpha-OH percentage was decreased 60%, and the conversion ratio of 11 beta-OH was more 1.29-fold than that of the mycelia. It affected the percentage of hydroxylation products when 2 mg/mL polyoxins were added into the mycelia conversion system.

17-alpha-Hydroxyprogesterone↗

PubMed2001

[The species and the distribution of officinal nettles in Sichuan Province].

OBJECTIVE: To study the resource of officinal nettles in Sichuan. METHODS: Investigating at the wild spots and consulting the relating specimens. RESULTS: It has been found that there are 11 spieces and subspieces of genus Urtica in Sichuan Province, of which 8 nettles are commonly used for treatment of rheumatism. CONCLUSION: This paper can provide scientific reference for further study of officinal nettles in Sichuan.

Anti-Inflammatory Agents↗

PubMed2001

[Retrograde interlocked intramedullary nailing of fresh humeral shaft fractures].

OBJECTIVE: To report the experience in retrograde intramedullary nailing of fresh humeral shaft fractures. METHOD: From February 1999 to June 2000, 32 humeral shaft fractures were treated with retrograde intramedullary nailing. All fractures were fresh and closed. The entrance point was on the proximal boarder of the olecranon fossa. Nails were locked distally and proximally. RESULTS: All fractures healed within 3 months and showed good function of shoulder and elbow joints. Radial palsy occurred in 1 patient post-operatively. CONCLUSION: Retrograde intramedullary nailing of humeral shaft fracture is one of the best treatments. Its advantages include no disturbance to the rotator cuff, stable fixation of the fractures, less soft tissue injury and fast recovery of the functions of near shoulder and elbow joint.

Adult↗

PubMed2001

Indomethacin attenuates post-suspension hypotension in Sprague-Dawley rats.

Orthostatic hypotension is a serious condition that is sometimes manifested in astronauts during standing postflight. These observations may be related to impairment of autonomic function and/or excessive production of endothelium-dependent relaxing factors. To evaluate the role of the cyclooxygenase inhibitor indomethacin as a countermeasure against the post-suspension reduction in mean arterial pressure (MAP), we examined the cardiovascular responses to 7-day 30 degrees tail-suspension and a subsequent 6-hr post-suspension period in conscious male Sprague-Dawley rats. Indomethacin (2 mg/kg) or saline were administered intravenously prior to release from suspension and at 2 and 4 hrs post-suspension. Direct MAP and heart rate were determined prior to suspension, daily and every 2 hrs post-suspension. During suspension, MAP did not change, in contrast, during post-suspension; it decreased compared to parallel non-suspended, untreated animals. There were no significant changes in heart rate. The reduction in MAP post-suspension was associated with significant increases in plasma prostacyclin. Indomethacin attenuated the observed post-suspension reduction in MAP and reduced plasma prostacyclin levels. Also, the baroreflex sensitivity for heart rate was modified by indomethacin--the MAP threshold for baroreflex activation was raised and the effective MAP range expanded. Thus, the post suspension reduction in mean arterial pressure may be due to overproduction of vasodilatory prostaglandins and/or other endothelium-dependent relaxing factors and alteration in baroreflex activity.

Animals↗

PubMed2001

[A preliminary study on relationship between Atractylodes lancea and A. chinensis as analyzed by RAPD].

OBJECTIVE: To study the relationship between A. lancea and A. chinensis by RAPD analysis. METHOD: Using 10 random primers, RAPD of seven Atractylodes samples was performed. The result was analyzed by RAPDistance Package Version 1.04. RESULT: A total of 60 polymerphic loci were amplified. It appears that the Atractylodes samples tend to cluster in relation to their habitats, and A. lancea has close phylogenetic relationship with A. japonica. CONCLUSION: A certain correlation among the chemical composition, genetic diversity and geographic distribution has been revealed. It is reasonable to separate A. lancea from A. chinensis according to their habitats.

Atractylodes↗

PubMed2001

[Appraisal of occupational stress and strain in primary and secondary school teachers].

OBJECTIVE: This study was conducted to assess occupational stress and strain in primary and secondary school teachers. METHODS: A test of occupational stress and strain was carried out by using Occupational Stress Inventory Revised Edition (OSI-R) in 1460 primary and secondary school teachers (teacher group) and 319 mental workers in non-educational area (non-teacher group as control). RESULTS: The results showed the level of occupational stress in role overload and physical environment in the teacher group was significantly higher than that in the non-teacher group (P < 0.05). In teacher group the level of occupational stress and strain increased with age; the occupational stress and strain in male teachers were significantly higher than those in female teachers (P < 0.01); the occupational stress and strain in secondary school teachers were significantly higher than those in primary school teachers. CONCLUSION: These results indicate: to protect and promote primary and secondary school teacher's health, particularly male teachers' health, to mitigate their work pressure and to raise the quality of education are important tasks in the area of occupational health.

Adolescent↗

PubMed2001

[Ultrasonic measurement of the elastic properties of goat condyles].

OBJECTIVE: The purpose of this experiment is to investigate the elastic properties of goat mandibular condyles and to provide basic constants for biomechanical study of temporomandibular joint. METHODS: A fresh goat mandible was adopted and the standard condyle specimen of 4 mm x 6 mm x 10 mm was obtained from it. The structure density of the condyle was determined according to Archimede's principle. The transmitting velocities of ultrasonic wave in different directions of goat condyles were measured by using the ultrasonic system, which was composed of a transmitting transducer of 1.25 MHz, a receiving transducer of 2.5 MHz, an ultrasonic analyzer (5052UA) and a digital storage oscilloscope (HP54601A). The elastic coefficients and technical constants could be calculated by elastic equations. RESULTS: The structural density of the sample and the wave velocities in different directions of the condyle were both obtained by direct measurement. And then, nine elastic coefficients and all of the technical constants of orthotropic condyles were calculated. CONCLUSION: 1. It is practicable to measure elastic properties of the condyle by using ultrasonic technique. 2. Goat condyles of the mandible are a type of anisotropic and linearly elastic material. 3. The direction perpendicular to the transverse ridge of the goat condyle has the highest Young's modulus. As to shear modulus, the direction along the transverse ridge shows the highest value.

Animals↗

PubMed2001

[The study of human papillomavirus(HPV) DNA expression in middle-ear carcinomas].

OBJECTIVE: To study the expressions of human papillomavirus DNA in the middle-ear carcinomas. METHOD: A PCR method using consensus primers for the detection of HPV6,11,16,18 was applied in 5 biopsies of middle-ear carcinomas. Eight biopsies of mucosa in middle-ear and mastoid were concluded as controls. RESULT: It was found that a resulting 80% (4/5) of the middle-ear carcinomas contained high-risk-type HPV DNA, while the 0%(0/8) of the controls. CONCLUSION: The high prevalence of high-risk-type HPV in carcinomas of the middle ear suggests that viral infections may be an important etiologic component in the carcinogenic process.

Adult↗

PubMed2001

[Simultaneous determination of chlorpheniramine, paracetamol and pseudo-ephedrine hydrochloride in composite tablets by high performance liquid chromatography].

An HPLC method is described for the determination of chlorpheniramine, paracetamol and pseudoephedrine hydrochloride simultaneously in Nuonuo composite tablets. Chromatographic conditions were: column, YWG-C18 column (150 mm x 4.0 mm i.d., 10 microns); mobile phase, 25% methanol(0.05 mol/L sodium dihydrogen phosphate); flow rate, 1.0 mL/min; detection wavelength, 205 nm; injection volume, 20 microL. The detection limits of chlorpheniramine, paracetamol and pseudo-ephedrine hydrochloride were 1.16 mg/L, 0.15 mg/L and 1.82 mg/L respectively and the corresponding average recoveries were 98.35% (n = 5, RSD = 1.60%), 101.16% (n = 5, RSD = 1.50%) and 98.50% (n = 5, RSD = 1.59%) respectively. The proposed method is simple, rapid and accurate and has been satisfactorily used for the quantitative analysis of real samples.

Acetaminophen↗

PubMed2001

[The clinic treatment of traumatic optic neuropathy].

OBJECTIVE: To observe the visual outcome of traumatic optic neuropathy treated with optic canal decompression surgery and medication. METHOD: 15 patients, suffering from traumatic optic neuropathy, had received medication and optic canal decompression surgery through the approach of ethmoid and sphenoid sinus. The vision acuity was divided into 5 grades: no light perception, light perception, finger movement, counting fingers, vision > or = 0.1, the vision acuity improvements reaching 2 grades or more were determined as effective. All patients were followed up for intervals of 1 month and 6 months. RESULT: The total efficient rate is 57.4%, no intraoperative or postoperative complications were encountered. Being followed up 6 months, 2 cases, who underwent respectively the operation 8, 11 days after trauma, felt no light perception. CONCLUSION: The approach through ethmoid and sphenoid sinus have the merits such as safety, less damage and larger operative field. Timely medication, megacorticosteroid therapy mainly, may play a good role in alleviating optic nerve edema and restoring its function. Comprehensive therapy may be an ideal treatment to traumatic optic neuropathy.

Adolescent↗

PubMed2001

[Detection of telomerase activity in laryngeal carcinoma and the association between telomerase activity and lymphatic metastasis].

OBJECTIVE: To investigate the telomerase activity in human laryngeal squamous cell carcinomas (LSCC) and the association between telomerase activity and lymphatic metastasis. METHOD: Telomerase activity was determined in 47 specimens of LSCC and 10 specimens of laryngeal inflammatory polyps using PCR-ELISA. RESULT: Telomerase activity was detected in 39 of 47 (83%) LSCC, but was not detected in all 10 laryngeal inflammatory polyps. There were significant differences between them (P < 0.01). Telomerase activation in LSCC with positive cervical lymph node involvement appeared to be higher (13 out of 13 cases, 100%) than in LSCC with negative cervical lymph node involvement (26 out of 34, 76.47%), (P < 0.05). CONCLUSION: Telomerase is activated frequently in LSCC and may become a biomarker of LSCC. The detection of telomerase activity in LSCC may have some value for predicting metastasis potential and prognosis and can guide the clinical practices applying selective neck dissection.

Adult↗

PubMed2001

[Determination of optical purity of SR-bioallethrin enantiomers by chiral high performance liquid chromatography].

A chiral stationary phase(CSP) was prepared by coating cellulose-tris (3,5-dimethylphenylcarbamate) onto aminopropylated spherical silica gel. On the CSP, the chiral separation of the bioallethrin enantiomers has been investigated and under the optimum conditions the optical purity of three samples of SR-bioallethrin enantiomers was determined by peak area. The results show that the method established is very ideal for determining the optical purity and evaluating the quality of the samples.

Allethrins↗

PubMed2001

[Factors of occupational stress on the working ability of petroleum workers].

The relationship between occupational stress and working ability and their influence factors have been studied. 1230 oil workers in 122 work types of oil industry were selected and an occupational stress questionnaire (OSQ) was filled. The working ability index (WAI) in the female was significantly higher than that in the male (P < 0.0001). The working ability of petroleum workers became lower with the increasing of age, the duration of working time and stress levels (P < 0.001). The correlation on the labor load, adverse factors in the working environment and WAI was negative. It was concluded that the occupational stress could decrease the working ability of petroleum workers.

Female↗

PubMed2001

[The study on effects of rhEGF on proliferation of human conjunctival epithelial cells].

PURPOSE: To investigate the effects of Recombinant Human Epidermal Growth Factor (rhEGF) on proliferation of human conjunctival epithelial cells and optimal dose in vitro. METHODS: Cultured human conjunctival epithelial cells were plated in 96-well plates at a density of 2,000 cells per chamber, treated with different concentrations of rhEGF (100 micrograms/ml, 50 micrograms/ml, 20 micrograms/ml, 10 micrograms/ml, 5 micrograms/ml and 1 microgram/ml). After 24, 48, 72 and 96 hours of treatment, cell viability was assessed with MTT assay. RESULTS: There was no significant difference in all experimental groups and the control group in the first 24 horus. Experimental groups at a concentration of 50 micrograms/ml, 20 micrograms/ml and 10 micrograms/ml had significantly higher cell viability than which of the control group 48 hours later. All groups of rhEGF had significantly higher cell viability than which of the control group after 72 hours treatment, except for 100 micrograms/ml of rhEGF. Each group of rhEGF after 96 hours treatment had higher cell viability than which of the control group, and 100 micrograms/ml of rhEGF had lower cell viability than which of the other experimental groups, with significant difference. CONCLUSION: rhEGF enhance proliferation of human conjunctival epithelial cells effectively in vitro. The optimal concentration ranged from 10 micrograms/ml to 50 micrograms/ml.

Cell Division↗

PubMed2001

[Immunity against Schistosoma japonicum induced by phage display peptides mimicking antigenic epitopes of Trichinella spiralis].

OBJECTIVE: To obtain the short peptides mimicking antigenic epitopes of Trichinella spiralis (T. s.), and explore their cross protective immunity against Schistosoma japonicum (S.j.) in mice. METHODS: IgG antibodies were purified from sera of mice infected with T.s.. The purified IgG was used to immunoscreen a phage random peptide library of 7 amino-acid residues displayed as a fusion to protein of filamentous phage. Positive clones were obtained by affinity selection, the reactivity of each clone binding to specific IgG was detected by ELISA. Kunming mice were immunized subcutaneously three times with mixed phage clones. The mice were sacrificed 45 days after challenge. The worms and the liver eggs were counted. RESULTS: After three rounds of panning, the relevant phages had been enriched approximately 150 times in production as compared to those from the first round. Of 24 phage clones randomly selected from the third round biopanning, 21 clones were shown to actually bind to the specific IgG. As compared with the control group, the worm and the liver egg reduction rates in vaccination group were 42.8% and 66.3% (P < 0.001), respectively. CONCLUSION: The above results demonstrate that antigenic epitopes of T. s. can be prepared by immunoscreening phage random peptide library and a significant protective immunity against S. j. can be induced by these epitopes in mice.

Animals↗

PubMed2001

[Cloning and characterization of three novel genes encoding transmembrane proteins of Schistosoma japonicum].

OBJECTIVE: To clone and analyze novel antigen molecules of Schistosoma japonicum (Sj), and to provide effective vaccine candidate antigens against schistosomiasis japonica. METHODS: Sj adult cDNA library was screened using sera of mice infected with Trichinella spiralis (Ts) and the inserts of positive clones were specifically amplified by PCR. The positive clones were sequenced and the sequence data were analyzed using Nucleotide BLAST software of NCBI and Expert Protein Analysis System of Swiss Institute of Bioinformatics. RESULTS: Nine positive clones were obtained after three rounds of immunoscreening. The size of these inserts ranged from 0.6 kb to 2.1 kb. Among five novel genes, Sj-Ts1, Sj-Ts3 and Sj-Ts5 (GenBank accession number: AY005816, AF299080 and AY024352, respectively) encode trans-membrane proteins with 83, 83 and 233 amino acids, respectively. Sj-Ts1 protein predicted contains one possible trans-membrance helix, one N-myristoylation site, two phosphorylation sites for protein kinase C and one for tyrosine kinase, Sj-Ts3 protein contains two possible transmembrance helices and one casein kinase II phosphorylation site, whereas Sj-Ts5 protein has five possible transmembrance helices, one N-glycosylation site, one N-myristoylation site, two phosphorylation sites for cAMP- and cGMP-dependent protein kinase and four for protein kinase C and one for casein kinase II. CONCLUSION: Three novel genes encoding three transmembrane proteins might be developed as new vaccine candidates against Sj infection.

Amino Acid Sequence↗

PubMed2001

[Basic assessment of trust level in medical apparatus and instruments sales systems by medical organization].

Purchase of medical apparatus and instruments is a strict work of taking the patient as a consuming objection. To analyze the trust level in medical apparatus and instruments sales systems and to set up the method of trust level assessment before the purchase, is an important link of ensuring the reality of medical products and consumerism. According to the principle mentioned about, the present article analyses the basic content of assessing factors and their inter-relationship, summarizes their expressions, studies and sets up the mathematical modal and it method. All these are of some value to medical organization in choosing factory owners and their products while the purchasing.

Commerce↗