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Biomedical subjects

M Walczak

Publications and source records attributed to M Walczak.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

Expression of stem cell markers on mononuclear cells derived from heparinized cadaveric organ donors before and after disconnection from the respirator.

We have attempted to evaluate the level of the earliest human hematopoietic cell marker expression (CD34, CD117, CD133, CD184) on cells obtained from heparinized cadaveric organ donors before and after disconnection from the respirator. Moreover, we compared various cell populations: (1) coexpressing CD34/CD117; (2) CD34/CD133; (3) highly enriched hematopoietic stem cells (CD34+CXCR4+CD45+); and (4) highly enriched tissue-committed stem cells (CD34+CXCR4+CD45-). Finally, we analyzed whether the level of hematopoietic stem cell marker expression depended on the age of the donor. The expression of the membrane receptors (CD34, CD45, CD117, CD133, CD184) was studied by flow cytometry. We observed that the proportion of mononuclear cells expressing these markers slightly decreased in bone marrow harvested after disconnection from the respirator compared with the samples obtained before disconnection. Moreover, the proportion of cells expressing CD117 antigen depended on age of the donor.

Antigens, CD↗
PubMedDate not supplied

Serum pancreatic polypeptide and glucagon immunoreactivity in fasting healthy and diabetic children.

Serum pancreatic polypeptide (hPP) concentrations ranged in normal children from 45 pg/ml to 525 pg/ml with mean value at 185 +/- 49 pg/ml. Both hPP and glucagon immunoreactivity (IRG) levels showed age-dependent decrease during childhood. In diabetic children plasma IRG concentrations were significantly increased in comparison with the healthy subjects while hPP concentrations were only slightly elevated. The age dependence of the hormones levels was completely effaced in diabetics. No significant serum hPP norm IRG values dependence on the duration of diabetes was found. IRG/hPP relations correlated with age in healthy children despite those in diabetic ones.

Adolescent↗