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Biomedical subjects

M Wagner

Publications and source records attributed to M Wagner.

At least 1,117 records · Page 62Linked to original sources

Oral satiety in the obese and nonobese.

Elapsed mealtime, number of mouthfuls per meal, and the time each mouthful was chewed were observed in thirty subjects, fourteen of whom were obese and sixteen, nonobese. Obese subjects consumed their meals and chewed mouthful in significantly less time than did nonobese subjects. There was no significant difference between the two groups in the number of mouthfuls per meal. In obese subjects, oral satiety as assessed in this study, appeared to play a less-than-meaningful role in the attainment of postprandial satiety. The clinical implications of these observations are discussed.

Adolescent↗

[Purification of Meerrettich-peroxidase by means of affinity chromatography on concanavalin A-agarose].

Crude preparations of horse-radish peroxidase were purified by means of affinity chromatography on Concanavalin A-agarose. The peroxydase was bound to Concanavalin A, whereas the majority of other proteins of the preparation pass through the column. Subsequently the peroxidase was eluted by means of 1 M sucrose with high purity. The purified enzyme is convenient for the immunoenzyme technique.

Buffers↗

Sindbis virus infected L cells studied by ferritin-labelled antibodies.

Sindbis virus infected L cells were examined by immunoferritin technique at different times after infection. Initially virus-specific antigens appeared diffusely distributed in the cytoplasm and at the surface of vacuoles. Later on, both labelled nucleocapsids surrounding vacuoles and mature virus within their lumen were demonstrated. Maturation of virus occurred predominantly at the periphery of cells, and that mainly by budding from cell processes. The appearance of big crystals consisting of nucleocapsids is a typical event of late stages of infection. These findings are compared with corresponding fluorescent antibody results.

Antigens, Viral↗