Search PubMed⌕ Search

Biomedical subjects

M Wagner

Publications and source records attributed to M Wagner.

At least 955 records · Page 53Linked to original sources
PubMed1985

[Results of Slocum-Larson patellar bridling].

The Slocum and Larson's surgical repair of the laterally dislocating patella is described. The results of 32 operations are discussed, follow-up-time was one to four years. 25 patients have no problems and have full activity in sports. Two patients have femoropatellar pain in the operated and two in the non-operated knee joint; so they had no normal athletic activity but they had no subluxation pain. One patient had a recent traumatic dislocation of the patella in landing a backward salto. We have obtained good results by the method of Slocum and Larson compared with various techniques of transferring tibial tubercle. This operation can be used also in young patients without disadvantages being a mere soft tissue procedure. The importance of lateral release and VMO-plastik is emphasized.

Adolescent↗
PubMed1985

[Follow-up management of recently operated capsule and ligament injuries of the knee joint].

The guidelines for the treatment of ligament injuries of the knee joint are subject to continuous changes due to new experiences in the field of functional anatomy and pathophysiology, as well as to the progressive changes in surgical treatment. The authors suggest answers to the questions of immobilisation, appropriate support, mobilisation, etc., questions which as yet have no final and binding solutions. They also discuss the problems of muscle atrophy and the time at which sportive activities of varying strains may be taken up again.

Combined Modality Therapy↗
PubMed1985

The influence of penicillin on growth and morphology of Streptococcus pyogenes in vivo.

The influence of penicillin (pc) on the growth, phagocytosis and killing of Streptococcus pyogenes was studied for an M protein positive (M+) and an M protein negative (M-) strain in vivo as well as in vitro. In vivo studies were based on a tissue cage model and the analyses were performed by CFU determinations and electron microscopic investigations. The M- strain was easily phagocytized with and without pc, but killing only occurred after pc treatment and thus the number of viable bacteria rapidly decreased under the influence of pc. M+ streptococci were not reduced in numbers by pc-treatment in vivo, but morphological changes and at high pc concentrations, phagocytosis could be seen. When this strain (M+) was cultivated in the absence of pc, the phagocytic cells were totally destroyed - a reaction that was prevented by penicillin. Variations in surface morphology of the two strains seem to influence the differences in sensitivity to penicillin, phagocytosis and killing.

Animals↗
PubMed1985

A sialic-acid-specific lectin from Cepaea hortensis that promotes phagocytosis of a group-B, type-Ia, streptococcal strain.

Group-B streptococci that possess a type-specific surface polysaccharide undergo phagocytosis only in the presence of antibodies to this, and complement. The snail Cepaea hortensis forms a lectin that is specific for sialic acid; treatment with this promoted the phagocytosis of a group-B streptococcus of serotype Ia (strain O90) in the absence of opsonic antibodies. The effect of the lectin was dose-dependent and required the presence of complement. The specificity of the lectin reaction for sialic acid was proved by the inhibition of phagocytosis by bovine submaxillary mucin. The participation of complement in the reaction was confirmed by demonstrating that C3 was bound to the surface of lectin-treated cells.

Animals↗
PubMed1985

Variations in binding of mammalian fibrinogens to streptococci groups A, B, C, E, G and to Staphylococcus aureus.

Twenty-eight beta-hemolytic streptococci of groups A, B, E, G and Streptococcus equisimilis as well as four Staphylococcus aureus strains were tested for their ability to bind fibrinogen preparations from different animal species: homo, baboon, rabbit, rat, guinea-pig, dog, horse, pig, cow and sheep. The patterns of binding indicated differences in the structures of the bacterial fibrinogen receptors. There were higher binding levels in streptococci groups A, G, and S. equisimilis than in representative group B and E strains. Considerable differences in the binding capacity were found within streptococci groups A and E. Group C and group G strains showed rather similar patterns and could be further divided into high-level and low-level binding strains. There is no correlation between binding levels of different animal fibrinogen preparations and the strains isolated from corresponding animals. Recent studies by others have shown that resistance to phagocytosis is mediated by fibrinogen-binding in streptococci group A. The existence of similar fibrinogen-binding structures in several streptococcal species indicates an important role with a definite survival value. It also suggests that M or T protein analogues are present in streptococci groups C, G and E.

Animals↗
PubMed1985

Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA.

Two genes from the family encoding mouse ribosomal protein S16 were cloned, sequenced, and analyzed. One gene was found to be a processed pseudogene, i.e., a nonfunctional gene presumably derived from an mRNA intermediate. The other S16 gene contained introns and had exonic sequences identical to those of a cloned S16 cDNA. The expression of this gene was demonstrated by Northern blot analysis of nuclear poly(A)+ RNA with cDNA and unique sequence intron probes. Each S16 intron contains a well-preserved remnant of the TACTAAC motif, which is ubiquitous in yeast introns and known to play a critical role in intron splicing. A sequence comparison with two other mouse ribosomal protein genes analyzed in our laboratory, L30 and L32, revealed common structural features which might be involved in the control and coordination of ribosomal protein gene expression. These include the lack of a canonical TATA box in the -20 to -30 region and a remarkably similar 12-nucleotide pyrimidine sequence (CTTCCYTYYTC) that spans the cap site and is flanked by C + G-rich sequences. The nature of the other members of the S16 family was evaluated by three types of experiment: a DNase I sensitivity analysis to measure the extent of chromatin condensation; an analysis of the thermal stability of cDNA-gene hybrids to estimate the extent of divergence of each gene sequence from that of the expressed gene; and a restriction fragment analysis which distinguishes intron-containing genes from intronless processed genes. The results of these analyses show that all genes except the expressed S16 gene are in a condensed chromatin configuration associated with transcriptional quiescence; that most of the genes within the S16 family have sequences greater than 7% divergent from the expressed S16 gene; and that at least 7 of the 10 S16 genes lack introns. We conclude that the ribosomal protein S16 multigene family contains one expressed intron-containing gene and nine inactive pseudogenes, most or all of which are of the processed type.

Animals↗
PubMed1985

Activation of factor XII-dependent pathways in human plasma by hematin and protoporphyrin.

Intravenous administration of hematin is effective in the treatment of acute exacerbations of the inducible porphyrias. In the course of such treatment, coagulopathies have occurred that are characterized by prolongation of prothrombin time, partial thromboplastin time, and formation of fibrin split products. In experiments in vitro with normal human plasma, we observed that hematin and protoporphyrin activated Factor XII-dependent pathways of coagulation and fibrinolysis, and that they generated kallikrein activity. Incubation of protoporphyrin with purified Factor XII resulted in activation as measured by amidolysis of a chromogenic substrate. Neither coproporphyrin, uroporphyrin, delta-aminolevulinic acid, porphobilinogen, or bilirubin activated Factor XII-dependent pathways. Exposure of serum containing added uroporphyrin, coproporphyrin, and protoporphyrin, but not hematin, to ultraviolet light (405 nm) resulted in activation of the classical pathway of the complement system. On the other hand, exposure of plasma containing uroporphyrin or coproporphyrin to ultraviolet light did not result in activation of Factor XII-dependent pathways.

Animals↗
PubMed1985

[Differentiated procedure in fractures of the coxal femur end in adults].

After own clinical observations a choice of surgical treatment in coxofemoral fractures is pointed out: Femoral neck fractures of stage Garden I will be stabilized by lag screws. For the rest of subcapital fractures we use lag screws or Smith-Peterson nailing for stabilization; only old patients without the possibility of mobilisation without weight-bearing after operation are treated by endoprosthesis of the hip. In Garden-IV-fractures and fracture-type Pauwels III indication for endoprosthesis is conditioned in most elderly patients. In basocervical and pertrochanteric fractures of younger patients a dynamic hip screw will be performed; in older patients we use elastic nails described by Simon-Weidner and Ender with good success. For stabilization of subtrochanteric fractures different operations dependent on type of fracture, bone-structure and general condition are used: elastic nails with or without wire-loops, interlocking nailing, Y-nail, dynamic hip screw, angled plate and compound internal fixation.

Aged↗
PubMed1985

Binding of horse-spleen ferritin to group A streptococci.

Receptors for horse-spleen ferritin were found on group A streptococci. Both electron microscopic and chemical investigation of Streptococcus cells treated with ferritin showed that M + variants of group A streptococci were able to bind substantially more ferritin than M - variants of the same serotypes. Ferritin receptors were located on the tops of filamentous protrusions of Streptococcus cell walls and only on the outer surface of isolated cell walls. Trypsin treatment destroyed the ferritin-binding capacity of streptococci completely, while mild pepsin treatment left the ferritin receptors undisturbed, or uncovered additional ones. The ferritin receptors were not identical with receptors for the Fc-portion of swine IgG. The finding of ferritin receptors on bacteria necessitates careful interpretation of results obtained by immunoferritin localization techniques.

Animals↗
PubMed1985

[Liver trauma].

Explore the source record for details and available documents.

Adult↗