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Biomedical subjects

M Wagner

Publications and source records attributed to M Wagner.

At least 595 records · Page 33Linked to original sources
PubMed1992

Hospital pharmacies watch prescriptions as debate rages over 'off-label' drug use.

Hospital pharmacies are closely monitoring physicians' prescribing practices in an effort to restrain drug expenditures by ensuring that drugs are used appropriately. But controversy continues over whether the increasingly common practice of using drugs for non-FDA-approved applications is appropriate and whether those uses should be allowed and covered by health insurers.

Cost Control↗
PubMed1992

Specificity of the sialic acid-binding lectin from the snail Cepaea hortensis.

The specificity of the sialic acid-binding lectin from the snail Cepaea hortensis, purified by affinity chromatography on fetuin-Sepharose, was studied by hemagglutination inhibition assay applying 32 sialic acid derivatives and 14 glycoproteins. 2-alpha-Methyl-9-O-acetyl-NeuAc was the most potent inhibitor, followed closely by 2-alpha-methyl-NeuAc and 2-alpha-benzyl-NeuAc. An axially orientated carboxyl group is a prerequisite for maximal lectin-sugar binding. Neither size nor polarity of the alpha-anomeric substituent significantly influenced inhibition potency. An intact sialic acid N-acetyl group is essential for optimal lectin-sugar interaction. The trihydroxypropyl side chain also is of great importance. However, a bulky hydrophobic substituent at the side chain like a 9-O-tosyl residue did not decrease binding to the lectin. The lectin did not distinguish between NeuAc alpha 2----3Gal beta 1----4Glc and NeuAc alpha 2----6Gal beta 1----4Glc. Among other sugars tested, only N-acetylglucosamine showed inhibition, although 50-fold less. The most potent glycoprotein inhibitors were those carrying O-chains only or preferentially, as ovine submaxillary mucin, bovine submaxillary mucin, and glycophorin A. Tamm-Horsfall protein was an exception being a strong inhibitor, although carrying only N-chains. Asialoglycoproteins were inactive. Glycoproteins containing the NeuAc alpha 2----3Gal sequence inhibited the lectin as well as those with NeuAc alpha 2----6GalNAc. From the results a model of the lectin's binding site for sialic acid is suggested.

Animals↗
PubMed1992

Latex gloves fingered in allergic reactions.

As more hospital workers don disposable latex examination gloves, reports of serious reactions and at least 16 deaths from latex allergies are being noted. Symptoms of latex allergy range from mild eczema to hives, welts and anaphylaxis. A rise in such incidents is of great concern for hospitals, which are expected to spend more than $500 million on latex examination gloves this year.

Gloves, Surgical↗