Biomedical subjects
Publications and source records attributed to M Wada.
Marked accumulation of I-123 IMP was noted in a patient with malignant melanoma. This preliminary finding suggests the potential role of I-123 IMP for detection and evaluation of the disease.
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To clarify the different actions of steroid hormones on calling and locomotor activity, minute pellets of steroid hormones were stereotaxically implanted into the third ventricle of castrated Japanese quail. Testosterone (T) pellets were effective in inducing calling to about 60% of that observed in castrated quail given subcutaneous implants of T. However, implants of 5 alpha-dihydrotestosterone (5 alpha-DHT) were completely ineffective and effectiveness of estradiol-17 beta (E2) was very slight, if any. On the other hand, E2 and T pellets enhanced locomotor activity; E2 was more potent than T, whereas 5 alpha-DHT was again ineffective. Cholesterol pellets had no effects on either behavior. Daily rhythms of calling and locomotor activity were also found in birds given ventricular T implants. These results indicate that T but not E2 is required for induction of calling and that aromatization occurs in the brain to exert enhanced locomotor activity. The results also indicate that changes in circulating T do not influence daily rhythms of calling and locomotor activity.
Hypothalamo-pituitary-thyroid activity varies with the temperature of the environment; we therefore measured variables involved with thyroid function in summer and winter in normal controls and in patients with primary hypothyroidism. All seven patients had impalpable thyroid glands and had received a set replacement dose of thyroxine for over a year. In the patients, serum T3 and FT4 levels were slightly but significantly lower in winter, and TSH levels and delta TSH at 30 minutes in the TRH tests were significantly higher. In the controls, there were no significant differences between summer and winter in these values. These findings suggest that the dose required for replacement of thyroid hormone in patients with hypothyroidism may be higher in winter than in summer.
When rat liver nuclei were digested with nuclease, we found that the chromatin-bound RNA polymerase II was liberated as two distinct complexes, peak 1 and peak 2, which seemed to reflect different functional states in cell nuclei. We further examined their occurrence in nuclear digests of various tissues of rats and the following results were obtained. Upon digestion with micrococcal nuclease of nuclei from brain, spleen, testis and kidney, chromatin-bound RNA polymerase II was liberated as two distinct forms which sedimented differently in a sucrose density gradient. The sedimentation rate of peak 1 varied depending on the tissue nuclei examined. After high salt or RNase treatment of the nuclear digests, peak 1 from liver, brain, spleen and testis nuclei showed the same sedimentation rate as did kidney peak 1, the rate for which remained unchanged by these treatments. The results suggested that peak 1 complexes from various tissue nuclei had basically the same structural organization, and we confirmed this by electrophoretic studies on RNase-treated liver and kidney nuclear digests. Peak 2 from various tissue nuclei exhibited identical sedimentation rates. Thus, the chromatin-bound RNA polymerase II seems to exist commonly in two distinct states in cell nuclei of rats.
Cells having the temperature-sensitive mutation groES131(Ts) were isolated from Escherichia coli K-12 strain C600T by thymineless death selection at 44 degrees C. This conditionally expressed mutation affected both cellular DNA and RNA syntheses at nonpermissive temperature, in addition to rendering cells unable to propagate phage lambda at permissive temperature.
Thirty-seven patients with peripheral vascular disease involving the legs were studied with first-pass peripheral radionuclide arteriography (RNA) and contrast arteriography to determine whether RNA could be used to evaluate perfusion of the toes. Data were acquired for 100 seconds using a multicrystal gamma camera following bolus injection of 30-35 mCi (1,110-1,295 MBq) of Tc-99m-labeled albumin during reactive hyperemia. Peripheral time-activity curves were examined and the time required for activity to decline to 75% of peak activity ( T75 ) was recorded. When an abnormal RNA was defined as T75 greater than or equal to 50 seconds, sensitivity was 92%, positive predictive accuracy 100%, and negative predictive accuracy 75%. These data suggest that peripheral RNA of the toes is a reliable, noninvasive procedure for evaluation of peripheral arterial disease; it can be valuable as a screening test prior to contrast arteriography as well as for follow-up.
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