Biomedical subjects
Publications and source records attributed to M W Weatherburn.
This report summarizes the results of eleven cross-Canada interlaboratory surveys between 1974 and 1985, for a small number of analytes. Statistical analysis of data for precision and accuracy by comparison to approved reference method values are presented.
A proposed reference method for serum creatinine has been developed under the auspices of the Committee on Reference Methods and Reference Materials of the Canadian Society of Clinical Chemists. A serum ultrafiltrate at pH 2.0 is applied through a closed sample loop injection system to a short column containing cationic resin of high resolving power. Elution with sodium citrate buffer by means of minipump at constant rate passes the eluate into alkaline picrate reagents in a continuous flow system (Technicon AAIII pump, AAII colorimeter 50 mm x 1.5 mm flow cell, narrow band width filter). The colour reaction peak is monitored visually to verify specificity and the area is calculated electronically. Specificity has been demonstrated by use of Jaffé-reactive substances such as glucose, sodium acetoacetate, L-ascorbic acid, pyruvic acid, L-dopa and glycocyamidine and also by use of an alternate colour reaction, sodium 3,5-dinitrobenzoate in place of alkaline picrate in the analysis of serum pools. Routine methods in common use, i.e., manual and automated alkaline picrate procedures, demonstrated a statistically significant high bias in interlaboratory studies in which this procedure was used for reference.
Explore the source record for details and available documents.
A compilation of hemoglobin values has been made from submissions from laboratories in Canada using the cyanmethemoglobin standard prepared and distributed by the Canadian Communicable Disease Centre (formerly the Laboratory of Hygiene). From 84 participating laboratories 21,580 values were analyzed statistically by age and sex. "Medical referrals", exclusive of blood dyscrasias, were included but were documented separately from "well persons". In most age groupings no significant difference in these two categories was found.Values for boys and girls were similar up to 12 years of age. For adult women from 18 to 84 years the mean value was 13.0 g. per 100 ml. (95% confidence limits 10.8-15.2 g.); for pregnant women 19 to 44 years the mean value was 12.2 g. per 100 ml. (9.7-14.6 g.). For men aged 17 to 24 years the mean value was 15.0 g. per 100 ml. (12.8-17.3 g.); 25-49 years 14.6 g. per 100 ml. (12.4-16.9 g.); 50-69 years 14.3 g. per 100 ml. (11.8-16.8 g.). It is noteworthy that for the most part the mean values were slightly lower than those frequently quoted as "normal" and that the mean values, particularly for the male, were lower with increasing age.
Explore the source record for details and available documents.
Explore the source record for details and available documents.