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Biomedical subjects

M W Taylor

Publications and source records attributed to M W Taylor.

At least 163 records · Page 9Linked to original sources
PubMed1971

New class of purine mutants of Chinese hamster ovary cells.

Mutants of Chinese hamster ovary cells (CHO/Pro(-)) were isolated after mutagenesis with N-methylnitrosoguanidine and selection by the bromodeoxyuridine technique. Six of these were mutants classified as purine-requiring. The metabolic block appears to be early in the purine biosynthetic pathway. The mutants do not appear to be genetically identical.

Animals↗
PubMed1971

Viruses as an aid to cancer therapy: regression of solid and ascites tumors in rodents after treatment with bovine enterovirus.

Treatment of ascites and solid tumors in mice (Sarcoma-1 and Ehrlich ascites carcinoma) with bovine enterovirus-1 resulted in regression of the tumors without any pathological effect on the animals. Death of mice with lymphatic-leukemia L4946 was delayed after such treatment. The oncolytic specificity of the virus does not appear to involve the production of interferon, but requires specific adsorption of virus to the tumor cells. The specificity of killing extends to cells in culture, since viral-transformed cells and oncogenic cells are susceptible to the virus, in contrast to cells of untransformed lines and cells of primary cultures, which are resistant. The possibility of utilizing the specificity of nonvirulent viruses in therapeutic treatment of human cancers is considered.

Animals↗
PubMed1971

Effect of double-stranded viral RNA on mammalian cells in culture.

During bovine enterovirus infection of Ehrlich ascites tumor cells, large amounts of double-stranded RNA accumulate. Addition of this double-stranded RNA to uninfected cells leads to rapid cell death. This is not a result of infectious virus production. Neither single-stranded RNA nor heat-denatured double-stranded RNA has this effect. Similar experiments with synthetic double-stranded polymers, poly(I).poly(C) and poly(A).poly(U), show that they are only slightly toxic at the concentrations used. The effect of the double-stranded RNA is nonspecific for cells of different origins. The implications of this finding in relation to the cytopathic effects of picornavirus and to cancer chemotherapy are discussed.

Animals↗
PubMed1969

Host-dependent restriction of mengovirus replication.

Mengovirus infection of a restrictive cell line, Maden's bovine kidney (MDBK), results in a virus yield 1,000-fold less than that obtained from productively infected cell lines such as L cells or Ehrlich ascites tumor cells (EAT). Cells of both types of host systems are infected with comparable efficiencies and are completely killed as a consequence of infection. Infective center assays, coupled with the observation of total cell killing, suggest that comparable numbers of cells synthesize viral antigen and release virus in both types of host system. Viral-specific ribonucleic acid (RNA) synthesis is initiated and proceeds in an identical fashion for approximately 4 hr after the infection of MDBK, EAT, or L-cells. At this time, viral RNA synthesis in MDBK ceases, whereas viral RNA synthesis in EAT and L-cells continues at a linear rate. These results indicate that none of the early viral events leading to the initiation of viral-specific RNA synthesis constitutes the primary site of mengovirus restriction in MDBK. Rather it appears that the cessation of viral RNA synthesis in restrictive cells constitutes the primary limiting event. Based on its delayed interaction with mengovirus RNA synthesis, it appears that the host-related restrictive agent is initially compartmentalized and then released as a consequence of infection subsequent to those early events in mengovirus infection leading to the initiation and continued synthesis of viral RNA.

Animals↗