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Biomedical subjects

M W Smith

Publications and source records attributed to M W Smith.

At least 343 records · Page 19Linked to original sources
PubMed1971

Ionic dependence of protein transport across the new-born pig intestine.

1. The everted small intestine of the new-born pig transports albumin to its serosal surface at rates which depend on the ionic composition of the bathing medium.2. Albumin transport takes place most rapidly at sodium and potassium concentrations of 120 and 6 mM respectively. Increasing the concentration of sodium or potassium, or decreasing the concentration of sodium, reduces the net transfer of albumin.3. Albumin can increase the serosal transfer of sodium, potassium and water. The calculated ionic composition of fluid transported in the presence of albumin closely resembles that presented to the mucosal surface over a wide range of sodium and potassium concentrations.4. Lowering the concentration of sodium reduces water transfer but only if albumin is present in the mucosal medium. Albumin-stimulated water transfer is only seen when the external concentration of sodium is high.5. Raising the concentration of potassium reduces water transport, whether or not albumin is present, but this reduction is too small to account for the inhibitory effect of potassium on albumin transport.6. The mutual interaction that exists between the transport of albumin and sodium is probably responsible for the increased potassium transport seen at low external concentrations of potassium. Higher concentrations of potassium appear directly to inhibit the interaction between the transport of sodium and albumin.

Albumins↗
PubMed1970

Effect of temperature acclimatization on the fatty acid composition of goldfish intestinal lipids.

1. The fatty acid composition of whole goldfish, whole-intestinal mucosa, intestinal mucosal membranes and individual phospholipids extracted from mucosal membranes were measured, fish adapted to different temperatures being used. 2. Alterations of the adaptation temperature did not noticeably affect the fatty acid composition of the whole-fish lipids, but there were marked changes in the fatty acids of lipids extracted from homogenates of goldfish intestinal mucosa. These changes were more pronounced in a membrane fraction prepared from these homogenates. Raising the adaptation temperature by 20 degrees C halved the percentage of C(20:1), C(20:4) and C(22:6) fatty acids and nearly doubled the percentage of C(18:0) and C(20:3) fatty acids recovered. 3. Choline phosphoglycerides constituted about one-half and ethanolamine phosphoglycerides about one-quarter of the total membrane phospholipids. 4. The fatty acids of choline and ethanolamine phosphoglycerides were more susceptible to temperature-dependent changes than were the phosphoglycerides of inositol or serine. 5. The increase in C(18:0) fatty acid that occurred in membranes of warm-adapted fish was greatest for ethanolamine phosphoglycerides, but increases also occurred in other phospholipid fractions and in membrane neutral lipids.

Acclimatization↗