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Biomedical subjects

M W Rodgers

Publications and source records attributed to M W Rodgers.

7 recordsLinked to original sources
PubMed1993

Microsomally associated heme proteins from French bean: characterization of the cytochrome P450 cinnamate-4-hydroxylase and two peroxidases.

Three heme-containing proteins of M(r) 57,000, 46,000, and 42,000 have been isolated from microsomes of elicitor-treated suspension-cultured cells of French bean. Following purification to homogeneity they were shown to be distinct with respect to amino acid and sugar compositions. The M(r) 57,000 protein proved to be the cytochrome P450 cinnamate-4-hydroxylase (CYP 73), by immunopurification and characterization. The M(r) 46,000 showed peroxidative activity toward guaiacol and all substituted phenylpropanoids tested and also gave a form of binding spectrum with cinnamic acid. The M(r) 42,000 had peroxidative activity toward guaiacol and a similar apparent binding spectrum to the M(r) 46,000 protein but was less active toward hydroxylated and methoxylated phenylpropanoids. The two peroxidases had cationic pIs and were distinct proteins rather than glycosylated or proteolytic variants.

Amino Acid Sequence↗
PubMed1992

Partial purification of Golgi-bound arabinosyltransferase and two isoforms of xylosyltransferase from French bean (Phaseolus vulgaris L.).

The purification of glycosyltransferases involved in wall matrix polysaccharide synthesis has been attempted. A number of activities readily demonstrated in isolated Golgi membranes are lost following detergent solubilization. However, solubilization releases pyrophosphorylases and phosphatases that hydrolyse the substrate in enzyme assays, whether UDP-glucose, -arabinose or -xylose is used. This hydrolysis, which cannot be completely inhibited, appears to be the major factor in the apparent loss of activity. Separation of this hydrolytic activity during further purification by ion-exchange and gel exclusion leads to recovery of glycosyltransferase activity. Thus two xylosyltransferases and one arabinosyltransferase could be partially purified. These appeared to be differentially expressed. The arabinosyltransferase of apparent M(r) 70,000 on size-exclusion chromatography was isolated from cells undergoing rapid growth and division. A xylosyltransferase of apparent M(r) 38,000 on size-exclusion chromatography was associated with cell expansion and primary wall synthesis. A second xylosyltransferase, which was purified to near homogeneity with M(r) 40,000, showed a peak of activity during the period of maximum secondary wall synthesis.

Chromatography, Gel↗
PubMed1991

L-phenylalanine ammonia-lyase from French bean (Phaseolus vulgaris L.). Characterization and differential expression of antigenic multiple Mr forms.

L-Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) purified from suspension-cultured cells of French bean (Phaseolus vulgaris) has been further characterized. A number of techniques, including use of an antiserum and affinity probes, have established that all the antigenic polypeptides represent polymorphic Mr forms of the enzyme. These peptides include an apparently higher-Mr (83,000) form which shows different kinetics of induction from the Mr-77000 forms that have been extensively characterized previously. The larger subunit appeared to be PAL by the following criteria: (a) binding to specific affinity and antibody matrices; (b) peptide mapping; (c) active-site labelling; and (d) amino acid composition. The increased Mr of the larger subunit was not completely attributable to glycosylation, although some sugar residues were detected in this Mr-83000 form but not in the other Mr forms. Mr-83000 subunits were also immunoprecipitated from translations in vitro of mRNA from cells that had been stressed for a long period. They were also detected in leaf tissues that were not yet undergoing an extensive wound response. This form of the enzyme may be constitutive and involved in the low-level accumulation of phenolics in most cell types. By contrast, the Mr-77000 forms of PAL were rapidly induced during elicitor action, wounding or cytokinin-induced xylogenesis as a key regulatory enzyme involved in the synthesis of phenolics under stress conditions or during differentiation.

Antigens↗
PubMed1987

Do angiotensin-induced changes in sheep renal venous blood and plasma composition indicate a reversal of glomerular filtration?

Angiotensin II markedly lowers the relative cell content of renal venous blood both with injection into the jugular vein and into the left ventricle of anaesthetised sheep; angiotensin I has a similar effect in this regard when given intravenously but the decreased cellular fraction is much less evident with left ventricular injection when the direct presentation to the kidneys precludes prior intra-pulmonary conversion to angiotensin II. The angiotensins also produce readily demonstrable alterations in renal venous plasma electrolyte, protein, creatinine and urea levels. This pattern of changes is related to associated intra-renal haemodynamics, reverse filtration of proximal tubular fluid, a possible anti-diuretic action of angiotensin I itself on the peritubular capillaries and acute renal failure in man and experimental animals.

Angiotensin II↗
PubMed1986

Implementing faculty practice: a question of human and financial resources.

Two issues confronting nursing education are: improving the relationship between nursing service and nursing education, and achieving status as an academic discipline. The concept of faculty practice addresses both of these problems. By definition faculty practice requires demonstration of clinical competence through practice, and scholarly outcomes of practice which satisfy the research requirement of the nursing faculty role. While scholarly practice by nursing faculty members may benefit the nursing service-education relationship, it is a myth that nursing faculty members were more effective teachers prior to nursing education's move into institutions of higher education. While few argue against the need for clinical competence of nursing faculty members for effective teaching, the need to attain educational preparation for effective teaching must not be overlooked. The vast majority of nursing faculty members hold master's degree as their highest earned credential. To establish itself as an equal in academe and to increase teaching effectiveness, nursing education needs faculty prepared at the doctoral level. If nursing faculty members add faculty practice and doctoral study to the standard faculty role requirements of teaching, research and service, chronic overload will result. Nursing faculty members cannot solve all the problems confronting nursing education by continuously increasing their workload. Nursing programmes need to assess their resources and priorities. For all of its merits, faculty practice should not be implemented until a programme has adequate resources to support it.

Clinical Competence↗
PubMedDate not supplied

Service learning. Resource allocation.

Service learning has the potential to increase student learning, assist communities, and provide faculty members with one focus area that brings teaching, service, scholarship, and practice together As nursing programs revise curricula to include community based service learning opportunities, the impact of these projects on material and human resources must be assessed. Planning can increase the probability of adequate resources, faculty productivity, and student learning. When planning service learning activities, addressing the following questions will assist in evaluating projects: Is there a the match between academic mission and community needs? What commitment will be made to the community? Will the project promote faculty productivity? Will it offer students superior learning opportunities? What resources will the project require?

Clinical Competence↗