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Biomedical subjects

M W Greaves

Publications and source records attributed to M W Greaves.

At least 361 records · Page 20Linked to original sources
PubMed1972

New in-vitro test for IgE-mediated hypersensitivity in man.

A new simple and sensitive in-vitro method for the diagnosis of type 1 (IgE-mediated) hypersensitivity in man is described. Sliced human skin is passively sensitized by reaginic serum from allergic patients and the presence of antigen-specific IgE on the sensitized slices is detected by assay of antigen-evoked histamine release. Serum from 12 out of 14 patients with clinical respiratory allergy and positive skin tests gave significant antigen-specific histamine release. This method, which is essentially an in-vitro model of the Prausnitz-Küstner reaction, should prove of value in the diagnosis of human reaginic hypersensitivity in man.

Adult↗
PubMed1972

Inhibition of prostaglandin biosynthesis by corticosteroids.

Since prostaglandins have been consistently recovered from a wide range of inflammatory reactions, including cutaneous inflammation, we have studied the effect of the anti-inflammatory corticosteroids hydrocortisone and fluocinolone on in-vitro biosynthesis of prostaglandins by skin. Skin homogenates synthesized prostaglandins E(2) and F(2)alpha in the presence of an excess of arachidonic acid substrate. Inhibition of biosynthesis of both these prostaglandins by corticosteroids was demonstrated. Since several members of the prostaglandin group of agents can reproduce all the cardinal features of inflammation and are found in a wide range of inflammatory reactions it is concluded that at least part of the anti-inflammatory properties of corticosteroids is due to inhibition of prostaglandin biosynthesis.

Animals↗
PubMed1972

Prostaglandin biosynthesis by human skin and its inhibition by corticosteroids.

Homogenates of human breast skin form prostaglandins E(2) and F(2alpha) in the presence of arachidonic acid substrate. Significant inhibition of biosynthesis of prostaglandin E(2) and F(2alpha) was observed in the presence of the anti-inflammatory corticosteroid fluocinolone acetonide. This effect was dose-related. By contrast no significant inhibition was observed in the presence of an approximately equimolar concentration of hydrocortisone.Since prostaglandins have been recovered from several types of inflammatory reactions and are capable of mediating most of the components of inflammation the possibility arises that corticosteroids may owe their anti-inflammatory activity at least in part to inhibition of prostaglandin biosynthesis.

Arachidonic Acids↗