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Biomedical subjects

M Villarroel

Publications and source records attributed to M Villarroel.

26 records · Page 2Linked to original sources

Characterization of Chilean hazel nut sweet cookies.

A series of studies were carried out to test the effect of the incorporation of Chilean hazel nut flour in sweet cookies at the levels of 0%, 5%, 10%, 15% and 20%. The proximate chemical analysis of the different flour mixtures showed a regular increase from 7.2 to 12.2%, 14.5% to 18.8% and 1% to 2.2%, respectively, decreasing at the same time with the percentages of water and carbohydrates. Chemical amino acid scores of leucine and threonine in wheat flour improved with the incorporation of Chilean hazel nut flour. The farinographic evaluation made to the different blends showed several changes occurred with the incorporation of Chilean hazel nut flour to wheat flour. These included increase in water absorption, decrease in dough developing time and weakening of the dough. Sensory characteristics such as appearance, texture, flavor and also acceptability improved with the incorporation of Chilean hazel nut flour into the cookie formulas.

Amino Acids↗

[Diagnosis and percutaneous drainage guided by ultrasonics in retroperitoneal abscess: experience with 16 cases].

The diagnosis of retroperitoneal abscess was made by ultrasound studies in 16 patients. Ultrasound guided percutaneous drainage followed in 10 patients confirming the diagnosis in all. A catheter was used in 8 and needle aspiration in 2. The 6 remaining patients were operated on. Patients with percutaneous drainage had better bacteriologic diagnosis and significantly shorter hospital stays than those who were submitted to immediate operation. Thus, ultrasound is an effective means for diagnosis and treatment of retroperitoneal abscess.

Abscess↗

[Protein complementation of defatted hazelnut flour with pea flour].

A great deal of research has been carried out to increase the availability of naturally-occurring proteins, or obtained from secondary products derived through industrial processes. As a result of the industrial exploitation of the native hazelnut (Gevuina avellana) in Chile, a defatted residue is obtained which eventually could be utilized as a human food. To determine the complementary potential capacity of the defatted hazelnut flour and pea flour, a study was carried out in weaning rats of the Wistar strain, as follows. The biological evaluation considered diet formulation with defatted hazelnut flour and pea flour in the 30:70, 50:50, 60:40 and 70:30 proportions, respectively. The NPR values obtained in this evaluation of the assay diets were: 3.4, 3.5, 3.9, 4.1 and 4.1, in comparison with 3.7 for casein. In percentage terms, the 4.1 NPR value was 11% higher than that obtained for casein. The maximum protein quality was observed when the two protein sources were mixed in the 60:40 and 70:30 (w/w) ratios. In regard to true digestibility, there were no significant differences among the experimental diets, but were lower than casein. The results of this study demonstrate that the defatted hazelnut flour constitutes an attractive nutritional alternative for the amino acid supplementation of cereal and legumes, the latter being of habitual consumption by the Chilean population.

Amino Acids, Essential↗

[Chemical composition and biological quality of defatted hazelnut flour].

The results of the chemical composition and biological quality of deffated hazel nut flour are shown. The samples analyzed contained significant amounts of proteins (19%) comparable to legume flour, higher than cereals and lower than deffated oleaginous flours. The oil extracted from the seed was analyzed and the average results obtained were the following: Refraction index, 1.47; saponification No. 184.8; iodine No. 85.0. The average composition of the fatty acids obtained by gas liquid chromatography was: Palmitic acid 2.3% Palmitoleic acid 37.0% Stearic acid 0.5% Oleic acid 39.5% Linoleic acid 6.9% Linolenic acid 1.1% Eicosanoic acid 2.3% Eicosaenoic acid 4.6% Docosenoic acid 3.4% Tetraeicosanoic acid 0.3% These results indicate a good-quality oil due to the low content of linolenic acid. The nutritive value of the deffated meal measured in the rats gave a net protein ratio (NPR) of 3.58, lower than the corresponding casein value (4.10). The true protein digestibility measured in the rat gave a value of 7.3%, compared to 95% for casein. The amounts of iron and phosphorous are comparatively lower than those reported for rape-seed meal and sunflower meal.

Animals↗

Enzymes as molecular automata: a stochastic model of self-oscillatory glycolytic cycles in cellular metabolism.

A stochastic model based on the molecular automata approach was developed to simulate the cyclic dynamics of glycolysis-gluconeogenesis in cell energy metabolism. The stochastic algorithm, based on Gillespie's method, simulates the master equation associated with any network of enzymatically controlled reactions. This model of the glycolytic-gluconeogenetic cycle was developed by assembling the stochastic kinetic reactions controlled by two enzymes: phosphofructokinase (PFKase) and fructose-1, 6-biphosphatase (FBPase). In order to obtain the hysteresis behaviour predicted by classical Sel'kov analysis, a minimum complexity is required in the allosteric regulations. This implies that the FBPase cannot have a single binding site for its transition to the inactive state (a minimum of two or three binding sites is necessary). Given the multimeric structure of this enzyme, this kinetic hypothesis is tenable. Other possible applications of the stochastic automata approach for different cases of channels, receptors and enzymatic control are suggested.

Animals↗

[Ultrasound in the diagnosis of congenital malformations of the brain].

After the work published by Kossof (1974), on the use of ultrasound for the study of trans-sectional cuts in the brain of newborns, the technic has been widely used in the exploration of this organ. This study evaluates the use of ultrasound in the diagnosis of congenital malformations of the brain in 11 patients seen during a period of 3 years at a regional hospital of southern Chile. The classification introduced by Beberly and Zimmerman was used in order to ascribe each case to a correspondent phase of organogenesis. Recent studies over the advantages of ultrasound for the detection of congenital malformations of the brain have resulted in the replacement of traditional invasive methods by ultrasonography for the evaluation of these problems.

Agenesis of Corpus Callosum↗

[Cerebral gigantism].

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Abnormalities, Multiple↗