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Biomedical subjects

M Vejtorp

Publications and source records attributed to M Vejtorp.

32 records · Page 2Linked to original sources
PubMed1982

Serodiagnosis of syphilis by an enzyme-linked immunosorbent assay for IgG antibodies against the Reiter treponeme flagellum.

An enzyme-linked immunosorbent assay for IgG antibodies against the flagellum (axial filament) of the Reiter treponeme (flagellum-ELISA) was developed and compared with the fluorescent treponemal antibody absorption (FTA-ABS) test and the Treponema pallidum immobilization (TPI) test with regard to diagnostic sensitivity and specificity. One serum from each of 827 individuals with and without syphilis was studied. In all diagnostic groups of syphilis there was no significant difference between the sensitivity of the FTA-ABS and the flagellum-ELISA, except in treated syphilis, where the FTA-ABS was more sensitive (P less than 0.01). In primary syphilis and in treated syphilis the sensitivity of the flagellum-ELISA was higher than the sensitivity of TPI (P less than 0.01 and P less than or equal to 0.05), respectively); in all other groups there was no significant difference between the sensitivity of TPI and flagellum-ELISA. The specificity of the flagellum-ELISA (99.0%) in 200 sera from blood donors without syphilis was not statistically different from the specificity of FTA-ABS (98.0%) and TPI (99.5%). The flagellum-ELISA seems to be well suited for routine serodiagnosis of syphilis and may replace other treponemal tests.

Antigens↗
PubMed1981

Solid phase anti-IgM ELISA for detection of rubella specific IgM antibodies.

IgM in the test sample was bound to anti-IgM on a solid phase consisting of a polystyrene microtest plate and rubella specific IgM antibody subsequently detected by incubation with crude rubella virus antigen and anti-rubella conjugate. High levels of rubella specific IgG, which decreased the sensitivity of an indirect ELISA for rubella IgM, did not interfere in the anti-IgM ELISA, whereas non-rubella IgM interfered by a competition for the anti-IgM on the solid phase. False-positive results owing to IgM rheumatoid factor could be prevented by the addition of aggregated IgG 0.1 mg/ml to the serum diluent. The median duration of the antibody response detected by the solid phase anti-IgM assay was 60 days (range 24-140) in test of serial serum samples from 17 patients with rubella. The assay appears suitable for routine diagnosis of postnatal rubella and will probably be particularly valuable for diagnosis of prenatal rubella.

Antibodies, Viral↗
PubMed1981

Rubella IgG antibody detection by ELISA using capillary blood samples collected on filter paper and in microtainer tubes.

Rubella IgG antibodies determined by ELISA of capillary blood samples collected on filter paper and in Microtainer tubes. The results correlated well to those found in tests of venous blood samples. Capillary blood samples can be collected with little inconvenience and by relatively untrained personel. Such samples seem therefore especially suited for large scale screening for rubella antibodies.

Antibodies, Viral↗
PubMed1980

Comparison of the sensitivity of ELISA and the haemagglutination-inhibition test for routine diagnosis of rubella.

A total of 500 paired sera were tested for rubella antibodies by the rubella IgG ELISA and the haemagglutination-inhibition (HI) test. Significant differences between the antibody level in the first and second samples of 417 serum pairs were not detected by either of the tests, whereas significant increases in 71 pairs were detected by both methods. A significant increase of the level in 12 serum pairs was demonstrated by the ELISA, but not by the HI test. The rubella IgG ELSIA is more sensitive than the HI test for diagnosis of recent rubella.

Antibodies, Viral↗
PubMed1980

Rubella IgM antibodies in sera from infants born after maternal rubella later than the 12th week of pregnancy.

The outcome of 338 pregnancies complicated by serologically confirmed rubella after the 12th week of gestation is reported. Therapeutic abortions were performed on 93 women, 1 pregnancy resulted in a spontaneous abortion and 5 in stillbirths. Cord blood or a sample collected at an age of 0--2 months from 209 liveborn infants were tested for rubella IgM antibodies after sucrose density gradient ultracentrifugation. Such antibodies were detected in samples from approximately 33% of infants born after maternal rubella in the 4th and 5th month of gestation, but only in 11% of infants born after maternal rubella later in pregnancy. The classical symptoms of congenital rubella were not observed with the possible exception of a suspected unilateral hearing loss in 1 infant born after maternal rubella in the 5th month of pregnancy.

Antibodies, Viral↗
PubMed1979

Diagnosis of postnatal rubella by the enzyme-linked immunosorbent assay for rubella IgM and IgG antibodies.

A semi-automated enzyme-linked immunosorbent assay (ELISA) was established for the detection of rubella IgM antibodies in non-fractionated sera. A cut-off level between rubella IgM positive and negative sera was determined by a study of sera without rheumatoid factor from 200 blood donors. Testing of 12 donor sera containing rheumatoid factor showed that 5 sera gave a positive result in the rubella IgM assay. Rubella IgM antibodies were quantified by ELISA in a study of 214 serial serum specimens drawn from 16 patients with rubella during a period of up to 10 years after the infection. Peak values of the IgM antibodies were reached approximately 8 days after onset of the rash, and the persistence of the IgM antibodies ranged from 17-90 days, with the exception of one patient with a prolonged IgM response. The rubella IgG antibodies increased slowly after the rash and reached maximum levels about 50-120 days, after which a monor decrease was observed. The results of the present study indicate that ELISA is suitable as a routine procedure for the serodiagnosis of recent rubella.

Acute Disease↗
PubMed1979

Enzyme-linked immunosorbent assay for determination of IgM rheumatoid factor.

A semi-automated enzyme-linked immunosorbent assay (ELISA) for determination of IgM rheumatoid factor was established. Human gammaglobulin (Cohn fraction II) was used as an antigen on a solid phase of polystyrene microtitre trays. The results were read on a spectrophotometer. ELISA and the sheep cell agglutination test were compared in a study of sera from 400 blood donors, 53 patients with active rheumatoid arthritis and of 200 sera received for routine determination of rheumatoid factor. The results of the two tests correlated well. The ELISA procedure was precise, objective, inexpensive and well suited for quantitative routine determination of IgM rheumatoid factor.

Adolescent↗
PubMed1978

Enzyme-linked immunosorbent assay for determination of rubella IgG antibodies.

A rubella virus antigen suited for enzyme-linked immunosorbent assay (ELISA) was grown in cultures of BHK/21/13 cells and purified and concentrated by membrane and ultrafiltration. This antigen was used in a semi-automated ELISA for determination of rubella IgG antibodies. The ELISA and the haemagglutination-inhibition (HI) test were compared in a study of 825 human sera. A close correlation was found between the results obtained by the two methods. Antibodies were, however, detected with ELISA in approximately 15% of the sera found negative in the HI test. The presence of antibodies was confirmed by the results of HI tests of the serum fractions of 19 sera separated by rate zonal ultracentrifugation. The EISA procedure employed in the present study was highly sensitive and allowed a precise quantitation of the antibodies by examination of one single serum dilution. ELISA was less time-consuming than the HI test and suited for routine laboratory use.

Antibodies, Viral↗
PubMed1977

Low concentration of plasma amino acids in newborn babies of diabetic mothers.

Plasma amino acid concentrations were measured in maternal peripheral vein and in umbilical vein and artery at birth (caesarean section) in 6 diabetic and 5 non-diabetic pregnancies. The mean birth weight in the control group amounted to 3.9 kg as oversize of the foetus in three cases contributed to the indication for caesarean section. The infants in the diabetic group consisted of "small for gestational age" babies (mean weight 2.8 kg). Free amino acid levels in the normal group and in diabetic maternal blood were in agreement with previous investigations. No difference in amino acid concentrations in the maternal plasma was found, but the concentrations of the umbilical vein plasma were significantly lower in the diabetic group. Foetal hyperinsulinaemia may be a cause of the low amino acid concentrations. Besides, abnormalities of the placenta and maternal vascular complications increase and the mean birth weights decrease significantly through the White classes. Conditions of impairment of placental transfer of amino aicds may thus be present. Characteristics features of the foetus may be consistent with the hypothesis as follows: The foetus in diabetic pregnancy is in varying degree exposed to an oversupply of glucose, hyperinsulinaemia, imbalanced uptake and a slightly diminished supply of amino acids.

Amino Acids↗
PubMedDate not supplied

Congenital hearing loss and rubella infection.

In an examination of Danish children aged 0 to 4 years, with congenital sensorineural hearing loss, 60% (39/65) had rubella antibody, compared with only 23% (17/72) in an age-matched control group. Based upon these results and similar investigations reported in the literature, it is concluded that serological testing for rubella antibody has proved to be of major importance in the evaluation of etiological factors of congenital hearing loss. Although diagnostic conclusions cannot be drawn on the basis of serological testing per se in the individual child, it is important to estimate the incidence of fetal rubella infection as the cause of congenital hearing loss, since this type of hearing loss may be prevented by active immunization.

Antibodies, Viral↗