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Biomedical subjects

M Vega

Publications and source records attributed to M Vega.

At least 91 records · Page 5Linked to original sources
PubMed1987

Development and evaluation of a low-cost amaranth (Amaranthus cruentus) containing food for preschool children.

A powdered blend for feeding preschool children was prepared from whole amaranth (Amaranthus cruentus) seeds, pearled oats, soybeans, sucrose and vegetable oil. The blend was similar in proximal chemical analysis and total caloric content, and identical in calorie distribution and vitamin and mineral patterns to a soy/oats infant formula previously developed. The amaranth blend equalled the FAO/WHO 1973 children's pattern in tryptophan and exceeded this pattern, as well as the soy/oats formula in all other essential amino acids; it reflected higher PER than casein and the soy/oats formula. In feeding trials with children 1-3 years of age, no significant differences in weight gain was observed when either product was fed. The anticipated cost of the amaranth blend is 6% lower than that of the soy/oats formula.

Amaranthus↗
PubMed1985

[Use of skull radiography in craniocerebral injuries in childhood].

Authors review the usefulness of radiographic skull examination in childhood head injury. Two hundred and forty-two patients assisted at the emergency area were prospectively recorded during one year. Data of filiation and epidemiology and symptoms and signs posed by Leonidas as "high risk criteria" to have a skull fracture, were registered. AP and L X-Ray skull were performed in all patients. Thirteen (5.37%) of them had a fracture. Blood discharge from the ear and skull depressed fracture were more frequently found in the fracture group with statistically significant difference (p less than 0.05 and p less than 0.01, respectively). The probability of positive finding was 8.8 for blood discharge from the ear, 17.6 for skull depressed fracture and 4.4 for bilateral black eyes. Because of no correlation between clinical and radiological findings, the radiographic skull examination must be systematically performed in every head injury if the purpose is to diagnose a fracture.

Adolescent↗
PubMed1985

Aberrant main left coronary artery in a patient with unstable angina.

A patient with significant obstructive coronary artery disease in an anomalous left main coronary artery arising from the right coronary artery is reported. The combination of this coronary anomaly with main left atherosclerotic disease is noteworthy and presented no surgical technical problem.

Aged↗
PubMed1985

Oesophageal atresia--severe tracheomalacia and its correction by aortopexy.

Tracheomalacia is an important cause of recurrent respiratory complications following correction of cogenital oesophageal atresia. It constitutes a well-defined syndrome, which requires clinical recognition and surgical correction in the most severely affected infants. Five cases are described. Special investigations confirmed tracheal collapse. Permanent relief of symptoms was achieved by suspension of the aortic arch to the sternum.

Aorta, Thoracic↗
PubMed1985

[Anthropometric characteristics of adolescents with primary epiphysiolysis].

The authors have studied five cases of adolescents, four girls and one boy, affected of slipped capital femoral epiphysis, aged between twelve and fourteen years. They describe some clinical particularities and the analytical findings. The anthropometrical studies confirm that slipped capital femoral epiphysis is an entity observed in adolescents with overweight and obesity. The parameter showing fat, at the braquial and body level, are over two standard deviation of the local normal patterns.

Adolescent↗
PubMed1982

Synthesis and composition of vitamin D-3 metabolites in Solanum malacoxylon.

The synthesis of vitamin D-3 hydroxylated metabolites in Solanum malacoxylon was investigated. When crude leaf homogenates and subcellular fractions were incubated with [3H]vitamin D-3 and [3H]25-hydroxy-vitamin D-3 under conditions described for animal vitamin D-3-25-hydroxylase and 25-hydroxy-vitamin D-3-1 alpha-hydroxylase, respectively, labelled metabolites identified on the basis of their chromatographic properties as 25-hydroxy-vitamin D-3 and 1,25-dihydroxy-vitamin D-3 were formed. Other unidentified product metabolites were also detected. Vitamin D-3-25-hydroxylase activity was localized in microsomes and 25-hydroxy-vitamin D-3-1 alpha-hydroxylase in mitochondria and microsomes. Chromatography of sterols isolated from leaf extracts preincubated with beta-glucosidase on Sephadex LH-20 columns permitted the isolation of three biologically active fractions with elution properties similar to vitamin D-3, 25-hydroxy-vitamin D-3 and 1,25-dihydroxy-vitamin D-3, respectively. Ultraviolet spectra characteristic of vitamin D-3 and its metabolites were obtained after purification of the fractions by TLC. Co-chromatography of individual fractions with authentic metabolites on TLC provided further evidence that the plant contains vitamin D-3, 25-hydroxy-vitamin D-3 and 1,25-dihydroxy-vitamin D-3 as glucoside derivatives. These results suggest that a similar pathway of vitamin D-3 hydroxylation as in animals may be operative in S. malacoxylon.

25-Hydroxyvitamin D3 1-alpha-Hydroxylase↗
PubMedDate not supplied

Prevention of stomal recurrence.

BACKGROUND: The authors reviewed the incidence of stomal recurrence (SR) in a population of laryngectomized patients to study associated risk factors and determine the usefulness of certain preventive procedures. METHODS: A retrospective study was performed in 296 patients with larynx and hypopharynx carcinoma treated by total laryngectomy. RESULTS: Of the 296 patients, 6 (2%) were initially seen with SR. In all cases, a risk factor was found to be involved: subglottic extension (5 cases), tracheotomy prior to laryngectomy (1 case). Since 1989, when preventive measures were systematically introduced in patients presenting risk factors, there have been no further cases of SR. The surgical measures in tumors with subglottic extension consisted of hemithyroidectomies, paratracheal lymph node dissection, and extensive tracheal resection; in the case of a tracheotomy prior to the laryngectomy, extensive resections of the tracheal stoma were carried out. CONCLUSIONS: The systematic use of preventive surgical measures together with postoperative radiotherapy to the stoma and superior mediastinum have led to a decrease in the appearance of SR.

Aged↗
PubMedDate not supplied

Optimised helper virus-free production of high-quality adeno-associated virus vectors.

BACKGROUND: Clinical development of adeno-associated virus (AAV) requires standardised, safe, efficient and scalable procedures for the manufacture of the rAAV vector, including production, purification and testing. Several strategies have been reported for the approach to the manufacturing problem. We report a helper virus-free process that produces high quality rAAV stocks. METHODS: rAAV were produced by triple transfection, a helper virus-free process. After lysis of the cells in the presence of nuclease, the rAAV produced were purified by HPLC through two ion-exchange columns in tandem followed by dialysis. rAAV stocks were thoroughly characterised for biological activity and for the presence of residual contaminants. The titer of infectious particles and of rep + particles was determined by dRA assay. Contaminating DNA and RNA were determined by fluorescent dye binding and real-time PCR. The protein content of the rAAV stocks was characterised by SDS-PAGE, ELISA test, Western blot and specific enzymatic assays for putative residual contaminating protein. The in vivo biological activity of the stocks was evaluated in mouse muscle. RESULTS: rAAV stocks obtained following this procedure elicit: 2-5 x 10(12) pp/ml; 3-6 x 10(10) ip/ml; < 10(3) rep + particles/ml; <0.3 mUeq/ml of residual benzonase activity; non-detectable Ad or beta-galactosidase proteins; <35 pg/ml of cellular genomic DNA; in vivo expression in mouse muscle without any immune reaction detected. CONCLUSIONS: This work demonstrates the possibility of producing purified high-quality rAAV free of helper virus. The procedure described in this paper is easily adaptable for large-scale production of clinical rAAV vectors.

Animals↗
PubMedDate not supplied

Immunogenicity of a Salmonella typhi CVD 908 candidate vaccine strain expressing the major surface protein gp63 of Leishmania mexicana mexicana.

Attenuated Salmonella typhi are attractive for use as live vector vaccines to express protozoal antigens and deliver them to the human immune system. The gene encoding the mature form of Leishmania mexicana mexicana gp63 under control of tac promoter was integrated into the delta aroC locus of the chromosome of attenuated delta aroC, delta aroD S. typhi strain CVD 908. After oral immunization of BALB/c mice with two 1 x 10(9) colony forming unit doses given 21 days apart, CVD 908 omega (delta aroC::Ptac-gp63) elicited a broad T cell-mediated immune response against L. m. mexicana gp63 as demonstrated by: (1) lymphoproliferative response to fixed whole L. m. mexicana promastigotes; (2) activation of IL-2 (but not IL-4)-producing lymphocytes; (3) appearance of cytotoxic T cells against mouse mastocytoma cells expressing gp63. This T-cell mediated immune response was associated with significant protection in F1 (BALB/cXC57Bl/6) mice challenged in their footpads with a wild type strain of L. m. mexicana.

Administration, Oral↗
PubMedDate not supplied

The interactivity between the CFTR gene and cystic fibrosis would be limited to the initial phase of the disease.

PURPOSE: Cystic fibrosis (CF) is a lethal genetic disorder affecting secretory epithelia, caused by mutations on the CFTR gene. In this paper we study the interactivity between the CFTR gene and CF disease over the time course of CF. METHOD: Cross-sectional analysis of CF patient population data from Latin-America, Canada, and The Netherlands, under the assumption that they represent stationary populations, was used to determined and correlates hazard rates, average cores and CF progression rates. RESULTS: Results suggests the existence of two phases throughout the course of CF. CONCLUSION: While the initial phase was related to the CFTR genotype, the kinetics of the second phase seems to be common to all groups considered. The hypothesis that the interactivity between the CFTR gene and CF disease would be limited in time is presented, suggesting that mutant CFTR would trigger a disease that evolves to become independent from the CFTR gene itself.

Cross-Sectional Studies↗