Search PubMed⌕ Search

Biomedical subjects

M Vazquez

Publications and source records attributed to M Vazquez.

67 records · Page 4Linked to original sources
PubMed1987

Treatment of childhood prednisone-resistant nephrotic syndrome and focal segmental glomerulosclerosis with intravenous methylprednisolone and oral alkylating agents.

Patients with prednisone-resistant nephrotic syndrome and biopsy-proven focal segmental glomerulosclerosis were treated with intravenous methylprednisone. After the first 2 weeks of therapy, the average urine protein excretion decreased from 247 to 96 mg/m2/h (p less than 0.04). Two of the 7 patients have had long-term, nearly complete remissions. The other patients relapsed. One relapsing patient was retreated with methylprednisolone and is now in remission. Four relapsing patients were treated with alkylating agents, in combination with methylprednisolone. All of these patients entered complete or partial remissions. Methylprednisolone causes a significant decrease in the proteinuria of children with focal segmental glomerulosclerosis. In addition, although the follow-up period is relatively short, it would appear that methylprednisolone, often in conjunction with an alkylating agent, has significantly improved the clinical status of these patients.

Administration, Oral↗
PubMed1982

Flavor preferences in malnourished Mexican infants.

Taste and flavor preferences were evaluated in malnourished (n=113) and well nourished (n=87) Mexican infants tested between 2 and 24 months of age. Not all infants were tested in all conditions. The malnourished infants all suffered from second or third degree malnutrition (marasmus) and were tested 2-28 days after admission to a renutrition center. Well nourished control infants were tested at their own homes or neighbor's homes. In the first experiment, testing consisted of brief presentations of moderate concentrations of sucrose (sweet), NaCl (salty), citric acid (sour) and urea (bitter). Infants were allowed to ingest as much of the taste substances as they wished. Volume of taste solutions and diluent was determined and provided the data for comparison between groups. In the second experiment, responses to casein hydrolysate and monosodium glutamate solutions were studied with soup serving as the diluent. The preferences of malnourished children for NaCl, citric acid and urea were not substantially different from those of the well nourished group. In sucrose tests, malnourished infants recently admitted to the renutrition center exhibited a decreased response. Malnourished infants ingested more casein hydrolysate and soup solution than soup alone. Control infants tended to respond in the opposite direction. All infants exhibited a preference for soup with MSG relative to plain soup.

Caseins↗
PubMed1982

Platelet adhesion to cultured vascular endothelial cells. A quantitative monolayer adhesion assay.

We have developed a sensitive in vitro method for measuring the adherence of platelets to cultured vascular cells. Human platelets, in citrated plasma, were radiolabeled with [3H]adenine to achieve a specific activity 100 to 1000 times greater than that obtainable with chromium-51 or [14C]serotonin. After exposure of cultured cell monolayers to radiolabeled PRP, nonadherent platelets were removed by a standardized wash procedure, and the number of adherent platelets calculated from liquid scintillation measurements. Significant release of platelet-associated radioactivity was not detectable after challenge with standard aggregating agents, and uptake of plasma (unincorporated) [3H]adenine by cultured cells was minimized by the addition of unlabeled adenine during the adhesion assay. Scanning electron microscopy, performed in parallel, allowed visual discrimination between platelet adhesion and aggregation as well as morphologic examination of the interacting platelets and cells. After incubation with PRP for 30 min, primary cultures of umbilical vein HEC bound less than 0.05% of added platelets (less than 1 platelet per cell). In contrast, virally transformed HEC showed increased platelet adhesion that was directly proportional to cultured cell density. Visual counts of the number of adherent platelets in scanning electron micrographs showed good agreement with calculated radiometric data. Comparative studies indicated that suspensions of washed, radiolabeled human platelets also can be utilized in this monolayer adhesion assay. This in vitro model system should facilitate study of the mechanisms of thromboresistance of vascular endothelium and its pathophysiologic alterations.

Adenine↗
PubMed1976

Role of heparin therapy in the outcome of adult hemolytic uremic syndrome.

Intravenous heparin therapy was associated with complete recovery of renal function in a young woman who developed adult hemolytic uremic syndrome while taking oral contraceptive agents. No controlled study is yet available but a review of an additional 36 reported patients who fulfill adequate criteria for the diagnosis of adult hemolytic uremic syndrome suggests that consideration should be given to a trial of heparin therapy in patients with this syndrome in whom renal biopsy shows recoverable glomeruli in the presence of persistent severe impairment of renal function.

Adolescent↗
PubMedDate not supplied

Average parameters as a trend to reduce the residual variability in bioequivalence trials.

Bioavailability and bioequivalence evaluations of drug products carried out using the experimental maximum concentration (Cmax) and the experimental time to reach Cmax (Tmax), are not advisable for slow-release formulations and for trials performed with saliva as biologic fluid. When slow-release curves are considered the drug concentration profiles usually show multiple peaks, making it difficult to compute a Cmax,Tmax value. The saliva profile throughout time shows a high variability observed as more than one peak in the saliva concentration versus time curves. In both cases, even if there is a major peak, when the statistical analysis of the data is performed, an important variability in Cmax results in high values in the residual variance of the ANOVA test. Consequently, the power of the bioequivalence test decreases and sometimes it is not possible to conclude on bioequivalence. The average concentration (Cav), the average maximum concentration (Cmax,av) and Cmax,av/Cav x 100 (%Cmax,av) are proposed in this paper as possible parameters in order to evaluate the profile of the concentration-time curves, as they reduce the residual variability in bioequivalence studies.

Analysis of Variance↗
PubMedDate not supplied

Effect of irradiation dose, storage time and temperature on the ESR signal in irradiated oat, corn and wheat.

Results obtained for electron-irradiated oat, corn and wheat kernels are discussed. The applied irradiation doses were up to 160 kGy. For doses up to approx. 50 kGy the number of free radicals produced by the irradiation is linear with the absorbed dose; moreover, the decay at room temperature in the dark or in the presence of light is quite similar. These facts point to the possible use of these kernels as dosimeters. An analysis of the free radical decay as a function of time and temperature shows the contribution of at least three types of radicals, whose half-lifes, radiochemical yields and activation energies are given.

Avena↗
PubMedDate not supplied

Surfactant production by neoplastic type II pneumocytes.

A bronchioloalveolar carcinoma of type II pneumocyte differentiation with abundant intraluminal secretion, histochemically and histologically mimicking alveolar proteinosis, is described. The tumor was of the diffuse type, involving almost the entire lower lobe of the left lung. The luminal aspect of the cells showed evidence of apocrine secretion. Ultrastructurally, the cells were characterized by the presence of cytoplasmic lamellar bodies. There were occasional cells with intranuclear tubules, and numerous rounded fragments of cytoplasm were present in the lumen as evidence of the apocrine activity. The intraalveolar secretion was composed of lamellar bodies in various stages of dissolution, tubular myelinlike networks characteristic of surfactant, and amorphous, granular material. This is the first reported instance of morphologic demonstration of surfactant secretion by a bronchioloalveolar carcinoma.

Adenocarcinoma↗
PubMedDate not supplied

Combined surgery to correct high myopia: iris claw phakic intraocular lens and laser in situ keratomileusis.

PURPOSE: To evaluate the results of combined surgery, implantation of an Artisan phakic iris claw intraocular lens (IOL) followed by laser in situ keratomileusis (LASIK) to correct high myopia. METHODS: A prospective study of 6 patients (8 eyes) with high myopia who had Artisan phakic IOL implantation followed by LASIK was undertaken. The IOL was a standard -15.00-D, 6-mm diameter optical zone. Residual refractive error was corrected by LASIK. Mean follow-up was 16 +/- 4 months (range, 12 to 20 mo). RESULTS: After the second procedure (LASIK), uncorrected visual acuity ranged from 0.4 to 0.63 (mean, 0.5 +/- 0.07) at 1 month and from 0.6 to 0.7 (mean, 0.62 +/- 0.04) at 12 months. Spectacle-corrected visual acuity improved 2 or more lines in 62.5% (5 eyes) from preoperative values. Mean postoperative spherical equivalent refraction was -0.68 +/- 0.23 at 1 month and -0.35 +/- 0.22 at 12 months after LASIK. All eyes were within +/-1.00 D of emmetropia following the LASIK portion of the two-stage procedure and 5 eyes were within +/-0.50 D. We had no major complications. No significant endothelial damage occurred. CONCLUSIONS: The accurate refractive outcome, absence of major complications, stability of results, and most important, improvement in quality of vision (defined as no change in vision when illumination conditions varied, eg, at night) experienced by these highly myopic patients are reasons to continue using and improving this combined technique.

Adult↗