Biomedical subjects
Publications and source records attributed to M V Zemskov.
Experiments were conducted on albino mice. Experimentally induced dysentery infection proved to become aggravated under the effect of yeast Na-RNA expressed in the increase of the number of animals which fell ill and perished. This phenomenon proved to be based on the acceleration of reproduction of the causative agent, with a simultaneous selection of its virulent clones seen both in vitro and in vivo.
An experiment on 58 rabbits demonstrated that only macromolecular agglutinins 19S are present in extracts from the lymph nodes after fractional immunization with small doses of typhoid vaccine or some dysentery vaccine. At the same time, antibodies in the blood serum are distributed in fractions of different molecular weight, starting from the 14th day of experiment. It is very probable that this distribution of antibodies is associated with the occurrence in the blood of an inhibitor of the activity of macromolecular antibodies.
The authors demonstrated a considerable, in comparison with the control, increase of the biomass of Sh. flexneri causative agent, when growing in meat-peptone broth with 1-4 mg of NaRNA, and of the number of colonies in the feces of the patients and of healthy persons artificially contaminated with Sh. flexneri when inoculated in Ploskirev's agar or Endo medium with 4 mg/ml of NaRNA. Using media with NaRNA for laboratory diagnosis of dysentery is recommended.
Allergization under conditions of functioning an inhibitory factor in the organism was studied by the reaction of leukocytolysis with Tsuverkalov's dysenterin and in the reproduction of hypersensitivity of the immediate type on the Schultz-Dale's model. It was shown that in functioning an inhibitory factor in the organism the degree of leukocytolysis reaction with the dysentery allergen decreased in the immunized animals in parallel with the fall in the serum antibody activity. A depression of the activity of macromolecular sensitizing antibodies was also observed.