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Biomedical subjects

M Uno

Publications and source records attributed to M Uno.

At least 145 records · Page 8Linked to original sources
PubMed1988

[Surgical treatment for hypertensive cerebellar hemorrhage--indication and characteristics of stereotaxic aspiration surgery].

From May 1976, through May 1985, eighty-nine patients with hypertensive cerebellar hemorrhage were admitted to our university hospital and affiliated hospitals. The age at onset ranged from 42 to 86 years, with a mean of 65.1 years. Thirty-one of these patients underwent conservative treatment, 20 were given ventricular drainage, 23 underwent suboccipital craniectomy and 15 underwent stereotaxic aspiration surgery. The patients were classified into four categories according to the grading of hypertensive cerebellar hemorrhage proposed by Matsumoto in 1982. Twenty-two cases were of benign type, 20 were moderate type, 30 were severe type, and 17 were fulminant type. The 22 benign type cases showed good recovery (ADL 1 or ADL 2), whereas the mortality rate of severe type cases was 26.7%, and that of fulminant type cases was 70.6%. The site and extension of hematoma were identified by CT. Fourty cases (45.0%) were confined to the left hemisphere, and 19 (21.3%) were localized in the vermis. When the hematoma volume was more than 15 ml, surgical evacuation of the hematoma was considered. Since 1981, stereotaxic aspiration surgery has been performed in cases of hypertensive cerebellar hemorrhage with a mean patient age of 66.9 years, ranging from 51 to 82 years. Patients treated have consisted of 2 with moderate type hemorrhage, 10 with severe type, and 3 with fulminant type, with an overall surgical mortality rate of 33.3%. However, the outcome of fulminant type hemorrhage has remained ADL 2 or ADL 3. The benefits of this type of surgery are that it is not only indicated as an emergency treatment for patients who are aged or at high risk, but that it can be also performed for fulminant type hemorrhage.

Aged↗
PubMed1987

The role of chromosome 15 in murine leukemogenesis. II. Relationship between tumorigenicity and the dosage of lymphoma vs. normal-parent-derived chromosomes 15 in somatic cell hybrids.

Somatic cell hybrids were generated between an MCF-virus-induced 15-trisomic T-cell lymphoma of AKR origin with a proviral insertion near the c-myc locus, and normal diploid fibroblasts or lymphocytes of CBAT6T6 origin. Three lymphoma/fibroblast fusions were performed. Six independently-derived clones from 2 fusions were tested for tumorigenicity. Three of the 6 clones were weakly malignant (take incidence 20% below), and 3 were strongly malignant (take incidence over 80%). All 3 lymphoma/lymphocyte hybrids and 6 derived clones were strongly malignant. All hybrids contained a nearly complete chromosomal complement of both parental cells. This was confirmed at the molecular level by determining the ratio of germ-line (G) vs. rearranged (R) myc-carrying Eco RI fragments that showed the expected 1.9-2.7:1 proportion. Malignant segregants selected from the weakly malignant lymphoma/fibroblast hybrids by in vivo inoculation showed changed 15-chromosome ratios. Four out of the 6 clones showed amplification of the lymphoma-derived 15-chromosome that carries the R-myc fragment and a concomitant decrease in the average number of the G-myc-carrying chromosomes. This was deduced from the fact that the G:R ratio was between 2 and 3:1 in the in vitro hybrids but became inverted (1:2-3) in the tumors. Two tumors showed no amplification of R-myc. G-myc was decreased. One of these tumors showed a change in the G:R ratio from 2.5:1.0 to 1.2:1.0, while the other was essentially unchanged (1.9:1.0 in the in vitro clone and 2.2:1.0 in the derived tumor). These findings support the notion that both the amplification of the lymphoma-derived 15-chromosome with the retrovirally rearranged c-myc carrying fragment and/or the loss of the G-myc-carrying 15-chr can contribute to the tumorigenic potential of the hybrids.

Animals↗
PubMed1987

In vitro production of human antibodies specifically reactive with human gastric cancer cells of established lines and autologous tissues.

Human lymphocytes derived from regional lymph nodes adjacent to the primary gastric cancer were transformed with Epstein-Barr virus (EBV) to establish lymphoblastoid cell lines secreting human antibodies reactive with cell surface antigens expressed on the gastric cancer cells. The EBV transformation technique was applied to lymph node lymphocytes obtained from 4 gastric cancer patients. As a result of mass screening with the radioactive cell binding assay for the production of anti-gastric cancer related antibodies, one culture (TGc-106) among 1,400 microcultures was identified to secrete human antibody specifically reactive with an established human gastric cancer cell line as target (MKN-45). Furthermore, it was demonstrated with the autologous assay system by the histoimmunofluorescence method that cell surface antigens of autologous gastric cancer cells could be clearly defined with human antibody from one culture (TEb-079) out of 470 microcultures established from a gastric cancer patient (GCP-26); there was no reactivity against the surrounding normal cells constructing the gastric wall. The immunoglobulin class of the human antibodies produced both in TGc-106 and TEb-079 was determined from immunodiffusion tests to be IgM.

Adenocarcinoma↗
PubMed1987

Effects of angiotensin I converting enzyme (ACE) related substances upon the vascular prostacyclin generation.

We investigated the interaction of angiotensin I (AI) converting enzyme (ACE) related substances to prostacyclin (PGI2) generation and ACE activity using cultured human vascular endothelial cells (EC) and rat aortic rings (AR). In case of cultured EC, AI and bradykinin (BK) increased PGI2 generation and at the same time ACE released from EC, however angiotensin II (AII) did not show any effect on both of them. When the EC were pretreated by captopril both of basal PGI2 production and ACE activity were reduced but the enhancing effects on PGI2 generation by AI or BK were preserved. On the other hand in the experimental system with AR, not only AI or BK but also AII increased PGI2 generation and the higher concentration of captopril inhibited PGI2 generation. When the EC were removed, the enhancing effect by AII was diminished and the mechanical stimulation to AR remarkably increased PGI2. From these experimental studies, we obtained the results that (1) the reported hypothesis that the vasodilative effect of captopril would be developed by the enhanced PGI2 generation was not confirmed in our systems of EC and AR. (2) AII did not affect on PGI2 generation of EC. Therefore the enhanced PGI2 generation by AII in AR was considered to be mainly derived from the mechanical stimulation to EC by the AII induced smooth muscle cells contraction. (3) It was speculated that the enhanced PGI2 generation by AI or BK might be modulated through their activating effect on ACE as an autoregulatory mechanism.

Angiotensin I↗
PubMed1987

[The problem of student suicide].

This study is a psychiatric survey of the student suicide at the Tokyo University. Counting the cases reported as such to the university administration, a total of 65 students committed suicide during the 20 years from 1962 till 1981. This amounts to a yearly average of 25.6 cases per 100,000 students. A comparison of this quota with that of several Japanese and foreign universities is made. In 1970, the year in which the students unrest at the Japanese universities reached its peak, the frequency of the suicide fell to its all time low. A possible explanation of this phenomenon is proposed. Out of the 65 students who committed suicide, 25 (38%) have at least once visited the psychiatric clinic of the Tokyo University. In view of the high percentage of the mentally disturbed students in the total number of suicides, the importance of measures taken of foster the mental health of the students is stressed for reasons of suicide prophylaxis. The issue in most of these cases was the conflict between the required scholastic accomplishments and the failure of achieving them. The resulting frustration becomes the immediate motive of the suicide. Therefore, a more intensive psychiatric care of such students is recommended especially during the examination season from January till May, when in Japan the students are exposed to the highest psychological stress. Out of 25 mentally disturbed students who ended by committing suicide, 18 revealed their desire to die in a more or less expressed way already during the time of their psychiatric treatment or consultation.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1987

Effects of angiotensin I converting enzyme (ACE)-related substances on prostacyclin generation and ACE activity of human vascular endothelial cells and rat aortic rings.

The effects of angiotensin I (ANG I) converting enzyme (ACE)-related substances on prostacyclin (PGI2) generation were investigated using cultured human vascular endothelial cells (EC) and rat aortic rings. ANG I or bradykinin (BK) increased while captopril decreased PGI2 generation and ACE activity of EC, whereas ANG II was without effect. The enhancement of PGI2 generation induced by ANG I or BK was not affected by the pretreatment with captopril. In rat aortic rings, ANG I, ANG II, and BK enhanced whereas captopril (10(-3) M) attenuated PGI2 generation. Mechanical stimulation of aortic rings stimulated PGI2 generation. These results suggest that (a) the conversion of ANG I to ANG II did not enhance PGI2 generation in EC; (b) the ANG II stimulated PGI2 generation of aortic rings was partially derived by mechanical stimulation of EC, probably originating from the contraction of smooth muscle cells by ANG II; (c) captopril directly inhibited PGI2 generation; and (d) in EC, the stimulation of PGI2 generation by ANG I or BK is probably regulated by an activating effect on ACE as an autoregulatory mechanism.

Animals↗
PubMed1987

Molecular characterization of novel reciprocal translocation t(6;14) in an Epstein-Barr virus-transformed B cell precursor.

An in vitro culture of FLEB14 cells, an Epstein-Barr virus-transformed B cell precursor containing the germ line immunoglobulin genes, gave rise to a uniclonally expanded variant, FLEB14 delta 3, which was rearranged at the immunoglobulin heavy-chain gene locus. Cytogenetic analysis showed that FLEB14 delta 3 had a novel reciprocal translocation, t(6;14)(q15;q32). Molecular cloning of the rearranged DNA fragments and determination of their nucleotide sequence revealed that the recombination event was reciprocal, imprecise, and nonhomologous and took place in the S mu region, like those found in Burkitt's lymphoma cells. We propose a molecular model to explain this genetic event which may be relevant to class switch recombination. The translocated sequence of chromosome 6 did not contain any known oncogenes, although the sequence is conserved among mammals. FLEB14 delta 3 did not show tumorigenicity.

B-Lymphocytes↗
PubMed1987

[Clinical studies on cefuzonam in obstetric and gynecological infections].

Seventeen patients with gynecological infections (5 cases each of Bartholin's abscess and adnexitis, 4 cases of endometritis and 1 case each of bartholinitis, pyosalpinx and vulvar abscess) were treated with cefuzonam (CZON, L-105) administered by intravenous drip infusion, 1-2 g twice daily, for 4 to 12 days (6 g to 23 g in total). The clinical effectiveness reached 94.1%, including 1 excellent case, 15 cases good, and a poor case. Bacteria were detected in 9 cases, and with CZON treatment, bacterial eradications were obtained in 6 case, but, in 3 cases, bacterial replacements were observed. No abnormal laboratory findings were noted. From the above results, CZON seemed to be a highly effective and useful agent for gynecological infections.

Adult↗
PubMed1986

Augmentation of synaptic responses in the dentate gyrus by daily electrical stimulation of the amygdala in cats.

When the amygdaloid complex was electrically stimulated, polysynaptic evoked potentials were produced in the ipsilateral dentate gyrus of the hippocampal formation. Twenty-four hours after tetanic stimulation of the amygdala, the dentate response to single test pulses was found to be increased in amplitude. Stimulation trains presented at a rate of 1/day had a cumulative effect on the dentate evoked potential, which attained 5-10 times the original response in amplitude. The results imply that the efficiency of synaptic transmission in the amygdalo-hippocampal pathway can be modified, associated with amygdaloid kindling.

Amygdala↗
PubMed1986

An Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA5) partly encoded by the transformation-associated Bam WYH region of EBV DNA: preferential expression in lymphoblastoid cell lines.

Four peptides were synthesized on the basis of amino acid sequences deduced from a highly spliced transcript encoded by the Bam W, Y, and H fragments of the Epstein-Barr virus (EBV) genome [Bodescot, M., Chambraud, J. B., Farrell, P. J. & Perricaudet, M. (1984) EMBO J. 3, 1913-1917]. Rabbit antisera against three of the four peptides identified a nuclear polypeptide that varied between 22 and 70 kDa in molecular size. Four of 20 EBV-positive human sera contained antibodies against this polypeptide. Since this is the fifth EBV-determined nuclear antigen (EBNA) discovered in growth-transformed cells, it is designated EBNA5. The antigen was detected in virus nonproducer lines (less than 0.01% EBV early antigen expression) and is thus not dependent on the viral cycle. It was differentially expressed depending on the origin of the lines. All 10 lymphoblastoid cell lines tested expressed EBNA5, but it could not be detected in 10 of 11 EBV-carrying Burkitt lymphoma lines. Infection of tonsillar lymphocytes with the B95-8 strain of EBV induced six EBNA5-specific polypeptides that varied between 41 and 70 kDa in molecular size with regular increments of 6 kDa. This may be due to the fact that the EBNA5 coding sequence includes the Bam W internal repeat. Parallel infection of the EBV-negative Burkitt lymphoma line Ramos with the same viral substrain did not induce detectable levels of EBNA5, nor was this antigen present in permanently EBV-converted Ramos sublines. These findings imply that the expression of the viral genome varies among B cells having different phenotypes.

Antibodies, Viral↗
PubMed1985

Biphasic modulation of spontaneous activities of entopeduncular neurons by systemic application of apomorphine in anesthetized cats.

The responses of entopeduncular neurons to apomorphine were investigated with extracellular recording methods in anesthetized cats. Apomorphine was applied intravenously in increasing doses, which cumulatively doubled with each addition (5-640 micrograms/kg). The spontaneous firing rates of entopeduncular neurons, which were inhibited by electrical stimulation of the caudate nucleus, increased when lower doses of apomorphine (5-20 micrograms/kg) were applied. However, higher doses (40-640 micrograms/kg) produced a dose-dependent decrease in the firing rate. The results suggest that there are direct and indirect effects of systemically administered apomorphine on entopeduncular neurons which may contribute to biphasic responses in the spontaneous discharge rate.

Action Potentials↗
PubMed1985

Production of antibody associated with non-A, non-B hepatitis in a chimpanzee lymphoblastoid cell line established by in vitro transformation with Epstein-Barr virus.

A continuous cell line of chimpanzee lymphocytes producing an antibody specifically associated with non-A, non-B hepatitis (NANB) was established. Peripheral blood lymphocytes of a chimpanzee convalescent from experimental infection with NANB hepatitis were transformed in vitro by Epstein-Barr virus infection into lymphoblastoid cell lines. Supernatants of the cell cultures were screened by immunofluorescence for antibody activity against the liver tissue of a chimpanzee with NANB hepatitis. Nineteen of the 1402 cultures were found to be positive for the activity. Ten of these 19 gave cytoplasmic reactions and the remaining 9 gave nuclear reactions in hepatocytes. One culture (48-1) stably producing the antibody was further characterized. The antibody produced in 48-1 was IgM and gave granular cytoplasmic reactions in hepatocytes. Cloning of 48-1 was performed by the soft agar method and cloned cell lines stably producing the antibody were obtained. The 48-1 antibody reacted with liver biopsy specimens from 12 chimpanzees obtained during the acute or chronic phase of hepatitis caused by five different NANB strains, but not with biopsy specimens from chimpanzees with hepatitis A or B or from normal chimpanzees. In addition, examinations of serial liver biopsy specimens obtained from 2 chimpanzees experimentally infected with NANB hepatitis demonstrated that the antibody reacted with the biopsies obtained during the preacute, acute, and chronic hepatitis, but not with those obtained before inoculation, early incubation period, or during convalescence. The present results indicate the specific association of the antibody with NANB hepatitis. Immunoelectron microscopy revealed that the antibody reacted with the microtubular aggregates identical to those previously described in a patient and chimpanzees with NANB hepatitis.

Animals↗
PubMed1984

Orthodromic and antidromic responses of nucleus accumbens neurons to stimulation of amygdala and substantia nigra in cats.

Response of nucleus accumbens neurons to stimulation of the amygdala and the substantia nigra were investigated extracellularly in anesthetized cats. They were excited by stimulation of the basolateral amygdaloid nucleus with latencies of 9.0-20.0 ms (mean 12.8 ms). About 70% of them were activated antidromically with latencies of 5.5-33.0 ms (mean 18.8 ms) following stimulation of the substantia nigra pars reticulata. The nucleus accumbens thus relays signals from the amygdaloid complex to the substantia nigra, and may act to bridge the limbic system to the basal ganglia.

Action Potentials↗
PubMed1981

[Development of clear zone after total hip replacement (author's transl)].

For studying the durability of Charnley-Müller's total hip prosthesis, 141 joints of the cases which had past at least 5 years from the operation were followed up by means of X-ray pictures to reveal the processes of CZ (clear zone at the interface between bone and cement) progress and loosening generation. Results 1) CZ which occurred once never disappeared and progressed in many cases. 2) The progress of CZ was supposed to be related to age and disease, but no relation was recognized with biomechanical factors. 3) The incidence of CZ was 83.7% on the acetabular side and 49.6% on the femoral side. 4) CZ of Stage I and II occurred within 1 year after the operation in many cases and CZ of Stage III and IV tended to increase about 3 years and 4 years after the operation, respectively, in the acetabular side, while in the femoral side Stage I occurred within 1 year in many cases, Stage II and III increased about 3 years after and Stage IV did about 4 years after. 5) The term for progressing from a stage to the next one considerably varied till Stage III, including non-progressive cases, but the term from Stage III to Stage IV was within 3 years in all the cases. 6) The durability of Charnley-Müller's total hip prosthesis observed in X-ray pictures was as low as 63.1%, 89 out of 141 joints 5 years after the operation, and it would be lower for a long time. The durability was particularly low in young cases of osteoarthritis. 7) The X-ray findings of loosening preceded clinical findings; thus precise interpretation of X-ray findings is useful for decision of prognosis and therapy.

Adult↗
PubMed1978

The mole of pallido-thalamic transmission investigated with intracellular recording from cat thalamus.

Pallido-thalamic transmission was studied by intracellular recording from neurons in the ventrolateral (VL) and ventroanterior (VA) nuclei of the thalamus in cats anesthetized with pentobarbital. Stimulation of the entopeduncular nucleus (ENT) produced short latency, inhibitory postsynaptic potentials in the VL-VA neurons (1.60 ms on average). When stimuli were applied closer to the VL-VA region along the pallido-thalamic pathway, i.e., to the rostral Forel's field, the IPSP latency was significantly reduced. Linear regression analysis of the IPSP latency against conduction distance between different stimulating the recording positions indicated that the IPSP was produced through a monosynaptic pathway at a conduction velocity of 5 to 11 m/s. The neurons which received IPSPs from the ENT distributed in the rostromedial VL and in the rostral VA, whereas relay cells responding only to the contralateral brachium conjunctivum were found in the caudal VL and in the dorsolateral portion of the rostral VL-VA complex. Reciprocal convergence of pallidal and cerebellar impulses were observed in only a small number of cells, which were located in the border between the two neuron groups. Recording of extracellular field potentials and focal stimulation within and around the rostral VL also indicated that the fiber potentials arose from the ENT nucleus and propagated along a bundle of fibers which terminated within the rostromedial VL-VA complex. These results are all explicable by assuming that the entopeduncular neurons are inhibitory in nature and so inhibit thalamic neurons monosynaptically.

Animals↗