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Biomedical subjects

M Umeda

Publications and source records attributed to M Umeda.

At least 397 records · Page 22Linked to original sources
PubMed1988

[Effect of metoprolol in patients with renal hypertension].

Eighteen patients with renal hypertension were treated with metoprolol (Seloken) for more than 3 months. Compared with the pretreatment value, the mean blood pressure decreased by 6.0, 11.2, 9.2 and 12.2 mmHg, after 2 weeks and 3, 6 and 12 months of treatment with metoprolol, respectively. All the differences observed were statistically significant (p less than 0.01). The blood pressure lowering effect of metoprolol was observed in 12 of the 17 patients (70.6%) examined. Decrease in plasma renin activity was seen in most of the responders. Although the number of patients was limited, the plasma level of metoprolol was also measured. The results indicated no sign of accumulation of metoprolol in the body. Moderate deterioration in renal function was observed in 1 patient but the treatment with metoprolol could be continued. Metoprolol administration had to be discontinued in 1 patient, who developed asthma. There were no other serious side effects.

Adult↗
PubMed1988

[Clinical efficacy of flucytosine on urinary candidiasis].

An antifungal agent (Flucytosine) was used to treat urinary candidiasis in 9 patients who had an indwelling catheter and developed fungal colony counts greater than 10(4). Among 9 patients with catheter drainage, urologic underlying diseases were benign prostatic hyperplasia in 7 and a neurogenic bladder in one patient all of whom had accompanied diabetes mellitus. Only one patient was supravesically diverted from the upper urinary tract through an indwelling catheter of bilateral ureterocutaneostomy after the removal of a tumorous bladder. All patients had previously received antimicrobials. Isolated strains of Candida were Candida albicans in 6, Candida tropicalis in 2, and Candida parapsilosis in one patient. Out of 9 patients having received daily administration of 1,500 mg Flucytosine for 2 weeks, 7 patients subsequently had no yield of fungal colony after the treatment. Minimum inhibitory concentration (MIC) of this agent was determined at the range of 0.1 to 0.2 microgram/ml in 5 patients with C. albicans and 0.2 microgram/ml in both patients with C. tropicalis. Otherwise, a high MIC of over 100 micrograms/ml indicating resistance to this agent was observed in only 2 patients with C. albicans and C. parapsilosis. Three of the 7 patients had recurrent urinary Candida infection even 2 weeks after the discontinuation of this antifungal therapy despite rapid and excellent eradication of urinary candidiasis. From these results, Flucytosine may be one of the most promising antifungal agent with a low MIC in the treatment of compromised urinary Candida infection and should be occasionally supplemented with a topical instillation of amphotericin B without any serious complication in the prevention of recurrence.

Aged↗
PubMed1987

Combined study of 1H-magnetic resonance imaging and depth-selected, EKG-gated 31P-magnetic resonance spectroscopy of the heart in vivo.

NMR is useful for both 1H-magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS). We undertook to combine these two merits of NMR for in vivo characterization of living rat heart in wide bore (9 cm) superconducting magnet under high magnetic field (6.4 Tesla). Spatial resolution of 1H-MRI attained 0.1 mm by spin warp method. Then, depth-selected, EKG-gated 31P-MRS was performed, adjusting the detection area to cover the heart that was identified by the preceding 1H-MRI. Three evidences that 31P-SMR signal chiefly originated from the heart without cross talk of adjacent organs indicated that combination of 1H-MRI and in vivo 31P-MRS under high magnetic field in whole animal is promising for more accurate evaluation of cardiac muscle metabolism.

Animals↗
PubMed1987

A simple metachromatic and fluorescent staining method for microorganisms using carbocyanine dye.

The cationic carbocyanine dye, 1-ethyl-2-[3-(1-ethylnaphtho[1, 2d]-thiazolin-2-ylidene)-2-methylpropenyl]-naphtho[1, 2d]thiazolium bromide, interacts with several classes of anionic polymers, exhibiting metachromasia. We were able to stain various kinds of microorganisms with this dye. Gram-negative bacteria were stained reddish purple, while Gram-positive bacteria were stained violet or bluish purple. Stains of molds were of various colors. Yeast vegetative cells were stained reddish purple, but zygotic asci were bluish. Chlamydia trachomatis inclusions, which are surrounded by cytoplasmic membranes, were also stained red. Microorganism and cell stains have different features and can be identified also by use of fluorescent microscopy. The new staining method we report here is rapid and simple enough for routine microscopical examinations of smears of clinical specimens including microorganisms.

Adult↗
PubMed1987

Anaplastic carcinoma of the thyroid with osteoclast-like giant cells. An ultrastructural and immunohistochemical study.

An unusual anaplastic thyroid carcinoma with osteoclast-like giant cells is reported in a 72-year-old woman. Monotonous proliferation of variously sized mononuclear tumor cells interspersed with numerous osteoclast-like multinucleated giant cells was dominant. A small area of papillary carcinoma merging with the anaplastic carcinoma was disclosed. Immunohistochemically, tumor cells forming papillary pattern were positive for both thyroglobulin and keratin. Cells and cell clusters positive for keratin were found in anaplastic carcinoma. Electron microscopically, the anaplastic carcinoma cells displayed prominent mitochondria, a rough endoplasmic reticulum, and intermediate cell junctions. The cell clusters formed irregular lumina into which numerous microvilli were extended and which contained colloid-like material. Occasionally incomplete basal laminae were present. Cell clusters showing transitional pictures from cell clusters to multinucleated giant cells and multinucleated giant cells engulfing mononuclear tumor cells were observed by both light and electron microscopy. Findings from this study support the conclusion that anaplastic tumor cells arise in preexisting differentiated thyroid cancer and that associated multinucleated giant cells are formed by the fusion of carcinoma cells.

Aged↗
PubMed1987

[Studies of enzymuria and proteinuria: Report 1: N-acetyl-beta-glucosaminidase in urological diseases].

N-Acetyl-beta-glucosaminidase (NAG) exists in renal tubules and seminal tract. We measured the NAG level in patients with urinary tract and seminal tract diseases and evaluated its clinical significance. The urinary NAG level was high in renal tubular function disorders such as glomerulonephritis, diabetic nephropathy and hydronephrosis. In these patients, NAG-isoenzyme studies indicated that the A-type isoenzyme decreased while the B-type isoenzyme increased. On the other hand, in the seminal tract disease patients, the urinary NAG level was high in benign prostatic hypertrophy with a catheter indwelling and in prostatitis. Urinary and seminal NAG-isoenzymes were measured in normal subjects. The isoenzyme patterns for urinary NAG and seminal NAG differed. The urinary NAG isoenzymes were measured in acute prostatitis patients, we found that the A-type isoenzyme decreased and the B type increased. In conclusion, when the A/B ratio of the NAG-isoenzyme is close to 24.9/75.0 in the urine, the presence of urological diseases in which seminal plasma could be mixed in, especially prostatic diseases, should be suspected.

Acetylglucosaminidase↗
PubMed1987

[The local blood flow of the rabbit bladder measured by electrochemically generated hydrogen gas].

The regional blood flow of the urinary bladder of rabbits was measured by electrochemically generated hydrogen gas. Then the effects of increased bladder capacity, and of systemic administration of scopolamine and neostigmine bromide on regional blood flow were examined. The local blood flow of the rabbit bladder was 75.9 +/- 8.4 ml/100 g/min (mean +/- S.E.) and it decreased by increased bladder capacity. It was also decreased by scopolamine bromide, while it was increased by neostigmine bromide.

Animals↗
PubMed1987

Transforming growth factor activity in pleural and peritoneal effusions from cancer and non-cancer patients.

Tests were performed to study the transforming growth factor (TGF) activity in samples of pleural fluid and ascites in patients with solid tumors and non-neoplastic diseases such as congestive heart failure and liver cirrhosis. The measurement of the activity was carried out in terms of colony formation in soft agar with NRK cells as indicator cells. When the fluid samples were directly assayed, 24% (4/17) of the cancer cases and 1 of the 4 control cases were positive. The eluate of Bio-Gel P-60 gel filtration was positive in all cases. beta-Type as well as alpha-type TGF activity was found in pleural and peritoneal effusions not only from cancer patients, but also from patients with non-malignant diseases.

Adult↗
PubMed1987

Pleiotropic effects of quercetin on the transformation of BALB 3T3 cells.

The effect of quercetin, a flavonoid, on the in vitro transformation of BALB 3T3 cells was examined. Quercetin at a low concentration (5 micrograms/ml) markedly suppressed the promoting action of 12-O-tetradecanoyl phorbol-13-acetate (TPA, 10 ng/ml) in this transformation system using 20-methylcholanthrene (MCA, 1 microgram/ml) as the initiator. At a concentration higher than 5 micrograms/ml, however, quercetin enhanced transformation by MCA. Quercetin itself was found to induce direct transformation weakly, but definitely, at a slightly higher concentration than that for enhancing MCA-induced transformation. These results indicate that quercetin has pleiotropic effects on the transformation of BALB 3T3 cells.

Animals↗