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Biomedical subjects

M Ueno

Publications and source records attributed to M Ueno.

At least 577 records · Page 32Linked to original sources
PubMed1980

Influence of unilateral adrenalectomy on renin-angiotensin-aldosterone system in primary aldosteronism.

In a study of 14 patients (11 Japanese and 3 white North Americans) with aldosterone-producing tumor carried out within 2 to 4 weeks after unilateral adrenalectomy, both plasma renin activity (PRA) and plasma aldosterone concentration (PAC) increased almost normally with short-term sodium depletion and upright posture. Aldosterone excretion rate (AER) also increased significantly with sodium depletion, and was normally suppressed by sodium loading. Highly significant correlations were observed between PRA and PAC (r=0.89, p < 0.001) or AER (r=0.88, p < 0.001) ater operation, whereas there had been no such correlation before operation. Either physiological or laboratory findings were normalized or markedly improved in all cases although pathohistological diagnosis was made as adenomatous hyperplasia in 10 out of 11. These results indicate that within 4 weeks of operation PRA and the remaining adrenal gland can almost normally respond to stimuli such as sodium depletion and upright posture regardless of the amount of spironolactone given preoperatively.

Adrenal Gland Neoplasms↗
PubMed1979

Circadian variation in urinary melatonin in clinically healthy women in Japan and the United States of America.

Urinary melatonin excretion is lower in East-Asian (Japanese) than in North-American (whites of mixed ethnic origin) women. Moreover, a statistically significant circadian rhythm is demonstrated by population-mean cosinor in the data pool from both groups of women. Furthermore, statistical significance characterizes interactions of effects from geographic differences (between ethnic groups) with temporal factors. Such spatio-temporal interactions await further scrutiny with a view inter alia of carcinogenesis as it is influenced by a spectrum of intermodulating rhythms.

Adult↗
PubMed1979

Analysis of human spermatozoal fertilizing ability using zona-free ova.

An in vitro fertilization assay employing zona-free hamster eggs was used to analyze human spermatozoal fertilizing ability. Human spermatozoa were preincubated for 18 to 20 hours in Biggers, Whitten, and Whittingham's medium (1971) at a concentration of 1 X 10(7) sperm/ml prior to the addition of zona-free superovulated hamster eggs. Eggs were examined microscopically 2 hours later for evidence of swelling or decondensing sperm heads in the cytoplasm. A total of 6266 eggs were examined in assays for both suspected fertile and infertile donors; 50 eggs/sample were examined. The percentage fertilization was found to range from 14% to 100% in the suspected fertile group with an average of 56.3%. The sperm concentration in this fertile group ranged from 22 to 303 million/ml with an average of 114. The suspected infertile samples yielded fertilization rates of 10% or less and an average count of 50.6 million/ml. These data suggest that human spermatozoa fuse with the vitelline membrane of zona-free hamster eggs and decondense with varying efficiencies. The percentage of fertilization in this cross-species system did not show a significant correlation with sperm concentration or motility. However, suspected infertile samples always yielded 10% or less fertilization in this assay. This method may have potential value as a diagnostic tool in evaluating human spermatozoal fertilizing capacity which avoids the ethical and logistcal problems associated with fertilization of human eggs in vitro.

Animals↗
PubMed1979

[Clinical experience with mezlocillin in infections of mouth and jaw (author's transl)].

The effectiveness and tolerance of the new semisynthetic acylureidopenicillin 6-[(R)-2-[3-methylsulfonyl-2-oxo-imidazolidine-1-carboxamido]-2-phenyl-acetamido]-penicillanic acid sodium salt (mezlocillin, Baypen) were investigated in 10 patients with bone and soft tissue infections of the mouth and jaw. The antibiotic was given in doses of 2 to 10 g/d. Indications for treatment were osteomyelitis of the jaw and/or phlegmon of the floor of the mouth and abscesses of the soft palate. 9 out of 10 infections could be cured. Due to the short treatment period one case was not assessable. In 6 patients 9 causative aerobic and anaerobic organisms were isolated which were eliminated. The clinical and laboratory control examinations did not reveal any adverse reactions.

Adolescent↗
PubMed1977

Inhibition of hamster sperm acrosome reaction and fertilization by oligomycin, antimycin A, and rotenone.

Effects of respiratory inhibitors (oligomycin, antimycin A and rotenone) on hamster sperm acrosome reaction and fertilization were studied. Hamster spermatozoa were incubated in a mixture of a modified Tyrode's solution and heat-treated human serum in the presence and absence of inhibitors. Oligomycin (2.4 x 10(-6) M), antimycin A (2.5 x 10(-6) M) and rotenone (2.5 x 10(-6) M) all reduced the incidence of the sperm acrosome reaction and fertilization without markedly affecting sperm motility. Antimycin A was the most effective in reducing the incidence of acrosome reaction. A reduction in the rate of fertilization was found in the presence of all of these respiratory inhibitors. The reduction in the incidence of acrosome reaction and fertilization by respiratory inhibitors implies an intimate relationship between high energy production (via respiration and oxidative phosphorylation) and capacitation and the acrosome reaction of spermatozoa. The necessity of oxidative metabolism for efficient capacitation and acrosome reaction of spermatozoa is suggested.

Acrosome↗
PubMed1977

Cell-mediated cytotoxicity in vitro of human lymphocytes against a cervical cancer cell line.

Peripheral blood lymphocytes from patients with uterine cervical cancer were tested against a cervical cancer cell line by micro-cytotoxicity assay. In stage O, high degree of cytotoxicity was observed but it decreased with progression of disease. In stage IV and recurrent cases, cytotoxic activity was negligible. Lymphocytes from healthy controls and myoma patients were not cytotoxic against target cells. On the other hand, lymphocyte response of phytohemagglutinin as a nonspecific T-cell activity was as high as the of healthy control in stage O, but the response decreased in stages I to IV and recurrent cases. In stages I to III, several cases showed cytotoxic activity but decreased response to phytohemagglutinin.

Carcinoma↗
PubMed1976

Alkyl streptozotocin analogues with improved biological activities.

Alkyl 16alpha- and -beta-glycosides of a series of N3-alkyl homologues of streptozotocin were synthesized from glucosamine hydrochloride. These compounds, when tested against ascites Sarcoma 180, Ehrlich ascites carcinoma, or leukemia L1210, exhibited potent antitumor activities, and antibacterial and diabetogenic activities were eliminated. Furthermore, the acute toxicities of these compounds were lower than that of streptozotocin. The methyl, ethyl, n-propyl, and n-butyl glycosides of streptozotocin, whether alpha- or beta-anomers, all showed higher antitumor activities than streptozotocin itself. The most active compound was found to be the methyl beta-streptozotocin.

Animals↗
PubMed1976

Immunotherapy of human lung cancer with BCG cell-wall skeleton.

Effect of BCG cell-wall skeleton (BCG-CWS) on the recovery of responsiveness of lymphocytes and the survival period was studied in patients with lung cancer. (a) Responsiveness of lymphocytes as demonstrated by proliferative response to phytohemagglutinin (PHA) or microcytotoxicity test with cultured cells of a bronchogenic carcinoma was depressed in the patients at later stages of cancer and it was restored by treatment with BCG-CWS in many of such patients. (b) Survival period of the patients at later stages such as III or IV was prolonged by treatment with BCG-CWS. Such an effect was detected even in the patients with carcinomatous pleuritis. (c) A close correlation was found between the reactivity such as PHA response or cytotoxicity and clinical course of the patients given BCG-CWS. Assay system with PHA response and microcytotoxicity test appears to be reliable in anticipating the prognosis and in following clinical course after the BCG-CWS treatment.

Adenocarcinoma↗