Search PubMed⌕ Search

Biomedical subjects

M Ueda

Publications and source records attributed to M Ueda.

At least 1,099 records · Page 61Linked to original sources
PubMed1989

Patent omphalomesenteric duct: a case report and review of Japanese literature.

Patent omphalomesenteric duct (umbilical enteric fistula) was diagnosed in a 7-day-old infant. The duct closed spontaneously, but at the age of 4 months the infant was readmitted for a resection of the duct. A review of the literature disclosed that 65 cases of patent omphalomesenteric duct have been reported in Japan. The male/female ratio was 2.8:1. Ten out of 36 infants (27.8%) were premature. Surgery was performed in as many patients as possible--55 out of 59 cases (93.2%). The ducts averaged 3.8 cm in length and 1.1 cm in diameter. Errant gastric mucosa was found in 3 out of 30 cases (10.0%). Prolapse of the ileum was present in 28 out of 53 patients (52.8%)--a relatively high incidence. Ten out of 55 patients died (18.2%); 8 of these had a prolapse of the ileum. In view of the high mortality rate of patients with a prolapse of the ileum and the strong possibility of intestinal obstruction, patent omphalomesenteric ducts should be resected surgically.

Female↗
PubMed1989

Establishment and characterization of CA 125 producing cell line (OMC-2) originating from a human endometrial adenocarcinoma.

A new human endometrial carcinoma cell line, designated OMC-2, was established from the endometrial adenocarcinoma of a 59-year-old woman. This cell line has grown well for 51 months and has been subcultured more than 50 times. Monolayer cultured cells are polygonal in shape, showing a pavement-like arrangement and a piling up tendency without contact inhibition. The chromosomal number shows aneuploidy and the modal chromosomal number is in the diploid range. The cells were transplanted into the subcutis of nude mice and produced tumors resembling the original tumor. 1 X 10(5) OMC-2 cells produced CA 125 (184-682 U) during 19 days in culture media. CA 125 was demonstrated immunohistochemically in the original tumor, heterotransplanted tumor, and OMC-2 cells. The cells contain no estrogen or progesterone receptors. Twenty-nine other reports of endometrial carcinoma cell lines are reviewed.

Adenocarcinoma↗
PubMed1989

[Antimicrobial activity of N-alkylethylenediamines against oral and other microorganisms].

Six N-alkylethylenediamines were synthesized and antimicrobial activity of each compound against oral microorganisms such as Streptococcus mutans, Actinomyces viscosus and Actinomyces naeslundii, and some others were determined in vitro. Dodecyl or tetradecyl derivative possessed maximum bacteriostatic activity among the test compounds. On the other hand, killing time of S. mutans in aqueous solution decreased with increasing alkyl-chain length.

Actinomyces↗
PubMed1989

A study on the hypotensive mechanism of pinacidil: relationship between its vasodilating effect and intracellular Ca2+ levels.

The direct effect of pinacidil on contractile protein systems and the relationship between its vasodilating effects and intracellular Ca2+ concentration were studied in isolated smooth muscle of guinea pigs. Pinacidil, nifedipine and hydralazine did not inhibit Ca2+-induced contracture in saponin-treated taenia caecum of guinea pig, while trifluoperazine and W-7 markedly suppressed it. Simultaneous determination of the intracellular Ca2+ concentration (fura 2 method) and tension development of the isolated femoral artery of guinea pigs showed that the intracellular Ca2+ level always decreased before vasorelaxation after pinacidil or nifedipine administration. Pinacidil showed no effect on cyclic AMP or cyclic GMP contents in rabbit aortic strips. These results indicate that pinacidil, like nifedipine and hydralazine, do not seem to directly act on the process from the formation of Ca2+-calmodulin to the actinmyosin interaction in the contracting mechanism of vascular smooth muscle, and that pinacidil relaxes the vascular smooth muscle by decreasing intracellular Ca2+-levels without elevating cyclic nucleotides.

Animals↗
PubMed1989

Effects of S-312, a new calcium antagonist, on the mechanical and electrophysiological responses of isolated cardiovascular preparations.

S-312, a new calcium antagonist with a bicyclic dihydrothienopyridine structure, potently relaxed the helical strips of various isolated rabbit arteries precontracted with high K+-depolarization, serotonin (5-HT) and U46619 (thromboxane A2 analogue), and it competitively inhibited Ca++-induced contractions in depolarized basilar and femoral arteries. These effects of S-312 were more potent than nifedipine and almost comparable to or slightly more potent than those of nicardipine. In comparison with nifedipine and nicardipine, the calcium antagonistic effect and the relaxant effect on 5-HT-induced contractions of S-312 were most prominent in the basilar artery. The potent vasodilating action of S-312 in the high K+-depolarized basilar artery was not easily reversed by washing. S-312 did not affect Ca++-induced contraction in the skinned fiber of guinea pig taenia caecum. The negative inotropic effect of S-312 in isolated guinea pig left atria was much less potent than those of nifedipine and nicardipine. S-312 above 10(-7) M preferentially increased AV nodal conduction time in Langendorff-perfused isolated rabbit hearts; and above 3 x 10(-8) M, it mainly decreased the maximum upstroke velocity of the action potential in isolated rabbit sinus node preparations. In summary, the present results indicate that S-312 is a potent new calcium antagonist possessing vasculoselectivity, especially for cerebral vessels.

Animals↗
PubMed1989

Effects of S-1389 (711389-S), a new antiarrhythmic agent, on the conduction in perfused guinea pig hearts.

In the His bundle and ventricular electrograms of Langendorff-perfused guinea pig hearts driven at a cycle length of 450 or 700 msec, S-1389 (711389-S), a new antiarrhythmic agent, above 3 x 10(-7) or 10(-6) M increased the basal conduction times in the following order: His-Purkinje system greater than ventricular and atrial muscles greater than atrioventricular (AV) node. Slowing of the ventricular and AV nodal conduction of extrasystoles with variable coupling intervals was also caused by S-1389. S-1389 above 10(-6) or 3 x 10(-6) M significantly prolonged the functional and/or effective refractory periods of the AV node and ventricle. Disopyramide (3 x 10(-6)-3 x 10(-5) M) also produced similar effects, but they were much less potent than those of S-1389. Although disopyramide did not produce the rate-dependent increases in the atrial and AV nodal conduction times and in the AV nodal refractory period, S-1389 increased these parameters rate-dependently.

Animals↗
PubMed1989

Effective administration of erythropoietin for renal anemia.

The erythropoietic effects of erythropoietin (EPO) have been investigated in the different administration schedule and injection routes. EPO was intravenously, intramuscularly or subcutaneously injected to partially nephrectomized anemic rats in 3 types of prescriptions (300 units of EPO per kg of body weight was respectively given in a dose at the first day, in 4 divided doses every 4 days, and in 7 divided doses every 2 days for 2 weeks). Repeated injections of EPO in divided doses caused stronger erythropoiesis than single injection. Especially, seven repeated injections promoted the strongest erythropoiesis. The serum iron concentration and reticulocyte counts suggested that erythropoiesis was continued less than 2 weeks after single injection but erythropoiesis in repeated injection groups had been accelerated for 2 weeks. Intravenous injections were less effective than either intramuscular or subcutaneous injections at 2 weeks after 7 repeated injections of 300 units of EPO per kg of body weight in 7 divided doses. Erythropoietic effects of EPO on the same total dose are dependent on the frequency of EPO injection and the durability of serum EPO concentration. On EPO usage, one bolus intravenous injection of excessive dose is considered to be wasteful and the repeating injections to maintain plasma EPO concentration is expected for the rational treatment of uremic anemia.

Anemia↗
PubMed1989

Factors influencing acute high-grade restenosis in emergency percutaneous transluminal coronary angioplasty for acute myocardial infarction.

We studied the factors which may induce acute high grade restenosis in emergency percutaneous transluminal coronary angioplasty (PTCA). PTCA was attempted in 50 patients with acute myocardial infarction, and the balloon catheter passed successfully across the occlusion site in 47 (94%) of the patients. These 47 patients were analyzed. "Acute restenosis" was defined as a lesion which was revascularized to less than 50% luminal reduction narrowed again to more than 75% luminal reduction 5 min after the balloon inflation. Univariate and multivariate analyses were used for determining factors which significantly influenced acute restenosis. The incidence of at least one restenosis episode was 45%. Multiple regression analysis selected 5 factors associated significantly with an increased rate of acute restenosis: 1) angiographic evidence of dissection, 2) lesion in the right coronary artery (RCA), 3) lack of or insufficient administration of thrombolytic agent preceding PTCA, 4) curved lesion and 5) relatively small balloon/artery diameter ratio. Acute restenosis correlated significantly with late reocclusion. This study indicates that it is important to administer a thrombolytic agent prior to emergency PTCA, and to use an adequately sized balloon to the artery when the acute restenosis occurs by using relatively smaller sized balloon. The present data also demonstrated that patients with RCA and a curved lesion have a relatively high risk of acute restenosis. This study indicates how patients with relatively high risk of acute restenosis may be identified.

Acute Disease↗
PubMed1989

[Relationship between clinical findings and subgingival microbial flora in periodontitis (2)].

The purpose of this study was to examine the relationship between clinical findings and subgingival relationship between clinical findings and subgingival microbial flora in periodontitis at the first medical examination and after initial preparation. The results obtained were as follows: 1. Clinical findings with the exception of plaque index showed improvement after initial preparation in comparison with the first medical examination. 2. In phase contrast microscopy, both total bacteria and incidence of spirochetes and motile rods decreased after initial preparation in comparison with the first medical examination. 3. Clinical findings with the exception of plaque index were related to the total bacteria and proportional distribution of spirochetes and motile rods in periodontal pockets, observed in phase contrast microscopy. 4. Total bacteria and proportional distribution of black-pigmented Bacteroides in periodontal pockets decreased after initial preparation in comparison with the first medical examination.

Dental Prophylaxis↗
PubMed1989

[Effectiveness of local delivery of ofloxacin using controlled-release strips (PT-01) in periodontal patients. Part 1. PT-01 application among treatment plans for periodontal disease].

Three different sites which have more than 5 mm pocket were randomly selected in each periodontal patient and were divided into three groups: PT-01 treated site, placebo treated site and control site. After application of either PT-01 or placebo twice for two weeks, subgingval scaling and root planing were performed. Then PT-01 and the placebo were applied weekly to the periodontal pocket for four weeks and clinical and microbiological evaluations were made. The results obtained were as follows: 1. On clinical evaluation, significant improvement was found in the PT-01 treated site in comparison with the placebo treated and/or control site. 2. On microbiological evaluation, slight improvement was found in the PT-01 treated site, whereas there was no significant difference among the three sites.

Delayed-Action Preparations↗
PubMed1989

[Effectiveness of local delivery of ofloxacin using controlled-release strips (PT-01) in periodontal patients. Part 2. Successive delivery system and combination with scaling].

Four different sites were randomly selected in each periodontal patient and divided into four group: PT-01-treated site (A), PT-01 treatment combined with scaling (S + A), placebo-treated site (P) and placebo treatment combined with scaling (S + P). PT-01 and the placebo were applied to the periodontal pocket weekly on days 0 to 28, and clinical and microbiological evaluations were made. The results obtained were as follows: 1. Weekly changes in plaque index, plus discharge and mobility of the tooth were not observed in any group. 2. In gingival index, bleeding on probing, pocket depth and gingival crevicular fluid, significant improvement was found in A in comparison with P and in S + A in comparison with S + P. 3. In total number of subgingival bacteria and in the ratio of motile rods and spirochetes to the total number of bacteria, significant reduction was found in A in comparison with P and in S + A in comparison with S + P.

Delayed-Action Preparations↗
PubMed1989

[Histopathological and clinical studies of acute necrotizing ulcerative gingivitis and evaluation of treatment].

We report the clinical data, light and electron microscopic findings, therapy and clinical course of three cases treated for acute necrotizing ulcerative gingivitis. The following results were obtained. 1. An ulcerous lesion was observed in the gingiva of all three cases, with case I also showing a depressed lesion in the ulcerous region. 2. In all three cases, the gingival sections displayed ulceration and showed fibrin deposition and neutrophil infiltration on the surface of the ulcerous region. Congested blood vessels and neutrophil infiltration also were observed in the connective tissue beneath the ulcerous region. 3. Many microorganisms, fibrin, cell debris, and enlarged intercellular spaces of the epithelium were seen on the surface on the ulcerous region. 4. Localized treatment, mainly plaque control, was found to be effective clinically and histopathologically.

Gingivitis, Necrotizing Ulcerative↗
PubMed1989

[Relationship between clinical findings and subgingival microbial flora in periodontitis (1)].

The purpose of this study was to examine the relationship between clinical findings and subgingival microbial flora in periodontitis. The results obtained were as follows: 1. In a phase-contrast microscopic study, no correlation was found between the clinical findings, total bacteria or proportional distribution of spirochetes or motile rods in the periodontal pocket. 2. Anaerobic incubation revealed no correlation between clinical findings, total bacteria or proportional distribution of black-pigmented Bacteroides in the periodontal pocket.

Humans↗
PubMed1989

Eosinophil hyporesponse of jirds induced by microfilariae of Brugia pahangi.

Male jirds (Meriones unguiculatus) were inoculated sc with 100 infective larvae of Brugia pahangi. After 16 weeks, the animals were reinoculated with a comparable number of organisms. Blood eosinophil responses during the 5 weeks subsequent to this attempt to reinfect were much lower than those of comparable naive animals, while the response to a heterologous infection (Toxocara canis) was comparable to that of controls. Mebendazole was given to infected animals for 2 weeks beginning 5 weeks (prepatent) or 16 weeks (patent) after infection. At comparable intervals after drug administration, the animals were reinoculated with infective larvae and the blood eosinophil response was measured over a 5 week period. The response in the animals treated during the prepatent period was higher than the untreated infected controls. Treatment during the patent period had no demonstrable effect. Jirds made artificially microfilaremic by intravenous inoculation of viable filaria before and after the standard infecting dose had a low eosinophil response to infective larvae. A primary experience of jirds with the microfilariae of B. pahangi evokes an eosinophil response. Subsequent inoculation of larvae did not produce a comparable response.

Animals↗
PubMed1989

Characterization and use of monoclonal antibodies directed against human erythropoietin that recognize different antigenic determinants.

We have established four hybridoma cells that produce monoclonal antibodies (MoAbs) R2, R4, R6, and R12 directed toward recombinant human erythropoietin (rHuEPO). MoAbs R2, R4, and R6 bound to EPO with high affinities (kd = approximately 2, 4, and 1 nmol/L, respectively) but MoAb R12 had a low affinity (240 nmol/L). These antibodies inhibited the biological activity of rHuEPO and EPOs from humans, rats, mice, and rabbits. This inhibition was due to the blocking of EPO binding to the target cells. The fully deglycosylated rHuEPO bound to the MoAbs, indicating that they recognized peptide sequences of the antigen but not the carbohydrates attached to the antigen. An immunosorbent column with the immobilized MoAb R2 was effective for the rapid purification of EPO. MoAb R6 bound to EPO at a site(s) different from those to which other MoAbs bound. Based on this finding, a sensitive and rapid enzyme-linked immunosorbent assay of EPO, in which EPO was sandwiched between two MoAbs (R2 and R6), was developed. The assay measured plasma levels of EPO as low as 5 mU/mL within several hours.

Animals↗